User:Neil J. Parikh/Lab Notebook

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
{{Template:BrowniGEM2008}}
 
<html xmlns:v="urn:schemas-microsoft-com:vml"
<html xmlns:v="urn:schemas-microsoft-com:vml"
xmlns:o="urn:schemas-microsoft-com:office:office"
xmlns:o="urn:schemas-microsoft-com:office:office"
Line 21: Line 20:
   <o:LastAuthor>Neil Parikh</o:LastAuthor>
   <o:LastAuthor>Neil Parikh</o:LastAuthor>
   <o:Revision>2</o:Revision>
   <o:Revision>2</o:Revision>
-
   <o:TotalTime>5</o:TotalTime>
+
   <o:TotalTime>4</o:TotalTime>
   <o:LastPrinted>2008-02-04T23:38:00Z</o:LastPrinted>
   <o:LastPrinted>2008-02-04T23:38:00Z</o:LastPrinted>
-
   <o:Created>2008-02-06T21:56:00Z</o:Created>
+
   <o:Created>2008-02-06T23:47:00Z</o:Created>
-
   <o:LastSaved>2008-02-06T21:56:00Z</o:LastSaved>
+
   <o:LastSaved>2008-02-06T23:47:00Z</o:LastSaved>
   <o:Pages>1</o:Pages>
   <o:Pages>1</o:Pages>
-
   <o:Words>236</o:Words>
+
   <o:Words>526</o:Words>
-
   <o:Characters>1348</o:Characters>
+
   <o:Characters>3004</o:Characters>
   <o:Company>Emory Vaccine Center</o:Company>
   <o:Company>Emory Vaccine Center</o:Company>
-
   <o:Lines>11</o:Lines>
+
   <o:Lines>25</o:Lines>
-
   <o:Paragraphs>3</o:Paragraphs>
+
   <o:Paragraphs>7</o:Paragraphs>
-
   <o:CharactersWithSpaces>1581</o:CharactersWithSpaces>
+
   <o:CharactersWithSpaces>3523</o:CharactersWithSpaces>
   <o:Version>12.00</o:Version>
   <o:Version>12.00</o:Version>
  </o:DocumentProperties>
  </o:DocumentProperties>
Line 37: Line 36:
   <o:RelyOnVML/>
   <o:RelyOnVML/>
   <o:AllowPNG/>
   <o:AllowPNG/>
 +
  <o:TargetScreenSize>1024x768</o:TargetScreenSize>
  </o:OfficeDocumentSettings>
  </o:OfficeDocumentSettings>
</xml><![endif]-->
</xml><![endif]-->
 +
<link rel=dataStoreItem
 +
href="iGEM%20Lab%20Notebook%20Neil%20Parikh_files/item0007.xml"
 +
target="iGEM%20Lab%20Notebook%20Neil%20Parikh_files/props0008.xml">
<link rel=themeData
<link rel=themeData
href="iGEM%20Lab%20Notebook%20Neil%20Parikh_files/themedata.thmx">
href="iGEM%20Lab%20Notebook%20Neil%20Parikh_files/themedata.thmx">
Line 45: Line 48:
<!--[if gte mso 9]><xml>
<!--[if gte mso 9]><xml>
  <w:WordDocument>
  <w:WordDocument>
 +
  <w:Zoom>90</w:Zoom>
   <w:TrackMoves>false</w:TrackMoves>
   <w:TrackMoves>false</w:TrackMoves>
   <w:TrackFormatting/>
   <w:TrackFormatting/>
Line 356: Line 360:
{font-family:"Cambria Math";
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;
panose-1:2 4 5 3 5 4 6 3 2 4;
-
mso-font-charset:1;
+
mso-font-charset:0;
mso-generic-font-family:roman;
mso-generic-font-family:roman;
-
mso-font-format:other;
 
mso-font-pitch:variable;
mso-font-pitch:variable;
-
mso-font-signature:0 0 0 0 0 0;}
+
mso-font-signature:-1610611985 1107304683 0 0 159 0;}
@font-face
@font-face
{font-family:Cambria;
{font-family:Cambria;
Line 512: Line 515:
text-indent:-.25in;
text-indent:-.25in;
mso-pagination:widow-orphan;
mso-pagination:widow-orphan;
-
mso-list:l16 level1 lfo12;
+
mso-list:l19 level1 lfo12;
tab-stops:list 0in;
tab-stops:list 0in;
font-size:10.0pt;
font-size:10.0pt;
Line 608: Line 611:
mso-fareast-font-family:"Times New Roman";
mso-fareast-font-family:"Times New Roman";
mso-hansi-font-family:"Lucida Grande";
mso-hansi-font-family:"Lucida Grande";
 +
mso-bidi-font-family:"Times New Roman";}
 +
p.MsoListParagraph, li.MsoListParagraph, div.MsoListParagraph
 +
{mso-style-priority:34;
 +
mso-style-unhide:no;
 +
mso-style-qformat:yes;
 +
margin-top:0in;
 +
margin-right:0in;
 +
margin-bottom:0in;
 +
margin-left:.5in;
 +
margin-bottom:.0001pt;
 +
mso-pagination:widow-orphan;
 +
font-size:12.0pt;
 +
font-family:"Arial","sans-serif";
 +
mso-fareast-font-family:"Times New Roman";
mso-bidi-font-family:"Times New Roman";}
mso-bidi-font-family:"Times New Roman";}
span.MsoIntenseEmphasis
span.MsoIntenseEmphasis
Line 855: Line 872:
mso-list-type:simple;
mso-list-type:simple;
mso-list-template-ids:-1763429746;}
mso-list-template-ids:-1763429746;}
 +
@list l4:level1
 +
{mso-level-tab-stop:.5in;
 +
mso-level-number-position:left;
 +
text-indent:-.25in;}
@list l5
@list l5
{mso-list-id:-128;
{mso-list-id:-128;
Line 973: Line 994:
text-indent:-.25in;}
text-indent:-.25in;}
@list l11
@list l11
 +
{mso-list-id:287706635;
 +
mso-list-type:hybrid;
 +
mso-list-template-ids:1137848646 -2109568246 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
 +
@list l11:level1
 +
{mso-level-number-format:roman-upper;
 +
mso-level-tab-stop:none;
 +
mso-level-number-position:right;
 +
margin-left:1.25in;
 +
text-indent:-.25in;
 +
mso-ansi-font-style:normal;}
 +
@list l11:level2
 +
{mso-level-number-format:alpha-lower;
 +
mso-level-tab-stop:none;
 +
mso-level-number-position:left;
 +
margin-left:1.75in;
 +
text-indent:-.25in;}
 +
@list l12
{mso-list-id:413170209;
{mso-list-id:413170209;
mso-list-type:hybrid;
mso-list-type:hybrid;
mso-list-template-ids:786721046 67698707 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
mso-list-template-ids:786721046 67698707 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
-
@list l11:level1
+
@list l12:level1
{mso-level-number-format:roman-upper;
{mso-level-number-format:roman-upper;
mso-level-tab-stop:none;
mso-level-tab-stop:none;
Line 982: Line 1,020:
margin-left:.75in;
margin-left:.75in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l12
+
@list l13
{mso-list-id:508057793;
{mso-list-id:508057793;
mso-list-template-ids:-259592024;}
mso-list-template-ids:-259592024;}
-
@list l12:level1
+
@list l13:level1
{mso-level-tab-stop:0in;
{mso-level-tab-stop:0in;
mso-level-number-position:left;
mso-level-number-position:left;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l12:level2
+
@list l13:level2
{mso-level-number-format:alpha-lower;
{mso-level-number-format:alpha-lower;
mso-level-tab-stop:.5in;
mso-level-tab-stop:.5in;
Line 995: Line 1,033:
margin-left:.75in;
margin-left:.75in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l12:level3
+
@list l13:level3
{mso-level-number-format:roman-lower;
{mso-level-number-format:roman-lower;
mso-level-tab-stop:.75in;
mso-level-tab-stop:.75in;
Line 1,001: Line 1,039:
margin-left:.1in;
margin-left:.1in;
text-indent:.65in;}
text-indent:.65in;}
-
@list l12:level4
+
@list l13:level4
{mso-level-text:"\(%4\)";
{mso-level-text:"\(%4\)";
mso-level-tab-stop:1.0in;
mso-level-tab-stop:1.0in;
Line 1,007: Line 1,045:
margin-left:1.0in;
margin-left:1.0in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l12:level5
+
@list l13:level5
{mso-level-number-format:alpha-lower;
{mso-level-number-format:alpha-lower;
mso-level-text:"\(%5\)";
mso-level-text:"\(%5\)";
Line 1,014: Line 1,052:
margin-left:1.25in;
margin-left:1.25in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l12:level6
+
@list l13:level6
{mso-level-number-format:roman-lower;
{mso-level-number-format:roman-lower;
mso-level-text:"\(%6\)";
mso-level-text:"\(%6\)";
Line 1,021: Line 1,059:
margin-left:1.5in;
margin-left:1.5in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l12:level7
+
@list l13:level7
{mso-level-tab-stop:1.75in;
{mso-level-tab-stop:1.75in;
mso-level-number-position:left;
mso-level-number-position:left;
margin-left:1.75in;
margin-left:1.75in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l12:level8
+
@list l13:level8
{mso-level-number-format:alpha-lower;
{mso-level-number-format:alpha-lower;
mso-level-tab-stop:2.0in;
mso-level-tab-stop:2.0in;
Line 1,032: Line 1,070:
margin-left:2.0in;
margin-left:2.0in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l12:level9
+
@list l13:level9
{mso-level-number-format:roman-lower;
{mso-level-number-format:roman-lower;
mso-level-tab-stop:2.25in;
mso-level-tab-stop:2.25in;
Line 1,038: Line 1,076:
margin-left:2.25in;
margin-left:2.25in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l13
+
@list l14
{mso-list-id:513572839;
{mso-list-id:513572839;
mso-list-type:hybrid;
mso-list-type:hybrid;
mso-list-template-ids:1063057342 67698713 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
mso-list-template-ids:1063057342 67698713 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
-
@list l13:level1
+
@list l14:level1
{mso-level-number-format:alpha-lower;
{mso-level-number-format:alpha-lower;
mso-level-tab-stop:none;
mso-level-tab-stop:none;
Line 1,048: Line 1,086:
margin-left:1.25in;
margin-left:1.25in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l14
+
@list l15
 +
{mso-list-id:654064786;
 +
mso-list-type:hybrid;
 +
mso-list-template-ids:-352949782 67698707 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
 +
@list l15:level1
 +
{mso-level-number-format:roman-upper;
 +
mso-level-tab-stop:none;
 +
mso-level-number-position:right;
 +
margin-left:.75in;
 +
text-indent:-.25in;}
 +
@list l16
{mso-list-id:661544068;
{mso-list-id:661544068;
mso-list-type:hybrid;
mso-list-type:hybrid;
mso-list-template-ids:893798754 67698707 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
mso-list-template-ids:893798754 67698707 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
-
@list l14:level1
+
@list l16:level1
{mso-level-number-format:roman-upper;
{mso-level-number-format:roman-upper;
mso-level-tab-stop:none;
mso-level-tab-stop:none;
Line 1,058: Line 1,106:
margin-left:.75in;
margin-left:.75in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l14:level2
+
@list l16:level2
{mso-level-number-format:alpha-lower;
{mso-level-number-format:alpha-lower;
mso-level-tab-stop:none;
mso-level-tab-stop:none;
Line 1,064: Line 1,112:
margin-left:1.25in;
margin-left:1.25in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l14:level3
+
@list l16:level3
{mso-level-number-format:roman-lower;
{mso-level-number-format:roman-lower;
mso-level-tab-stop:none;
mso-level-tab-stop:none;
Line 1,070: Line 1,118:
margin-left:1.75in;
margin-left:1.75in;
text-indent:-9.0pt;}
text-indent:-9.0pt;}
-
@list l15
+
@list l17
 +
{mso-list-id:725564168;
 +
mso-list-type:hybrid;
 +
mso-list-template-ids:-713246640 67698703 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
 +
@list l17:level1
 +
{mso-level-tab-stop:none;
 +
mso-level-number-position:left;
 +
text-indent:-.25in;}
 +
@list l18
{mso-list-id:780953595;
{mso-list-id:780953595;
mso-list-type:hybrid;
mso-list-type:hybrid;
mso-list-template-ids:-1008036830 67698713 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
mso-list-template-ids:-1008036830 67698713 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
-
@list l15:level1
+
@list l18:level1
{mso-level-number-format:alpha-lower;
{mso-level-number-format:alpha-lower;
mso-level-tab-stop:none;
mso-level-tab-stop:none;
Line 1,080: Line 1,136:
margin-left:1.25in;
margin-left:1.25in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l16
+
@list l19
{mso-list-id:875889331;
{mso-list-id:875889331;
mso-list-template-ids:1480984720;}
mso-list-template-ids:1480984720;}
-
@list l16:level1
+
@list l19:level1
{mso-level-style-link:"List Number";
{mso-level-style-link:"List Number";
mso-level-tab-stop:0in;
mso-level-tab-stop:0in;
mso-level-number-position:left;
mso-level-number-position:left;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l16:level2
+
@list l19:level2
{mso-level-number-format:alpha-lower;
{mso-level-number-format:alpha-lower;
mso-level-tab-stop:.5in;
mso-level-tab-stop:.5in;
Line 1,094: Line 1,150:
margin-left:.75in;
margin-left:.75in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l16:level3
+
@list l19:level3
{mso-level-number-format:roman-lower;
{mso-level-number-format:roman-lower;
mso-level-tab-stop:.75in;
mso-level-tab-stop:.75in;
Line 1,100: Line 1,156:
margin-left:1.0in;
margin-left:1.0in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l16:level4
+
@list l19:level4
{mso-level-text:"\(%4\)";
{mso-level-text:"\(%4\)";
mso-level-tab-stop:1.0in;
mso-level-tab-stop:1.0in;
Line 1,106: Line 1,162:
margin-left:1.0in;
margin-left:1.0in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l16:level5
+
@list l19:level5
{mso-level-number-format:alpha-lower;
{mso-level-number-format:alpha-lower;
mso-level-text:"\(%5\)";
mso-level-text:"\(%5\)";
Line 1,113: Line 1,169:
margin-left:1.25in;
margin-left:1.25in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l16:level6
+
@list l19:level6
{mso-level-number-format:roman-lower;
{mso-level-number-format:roman-lower;
mso-level-text:"\(%6\)";
mso-level-text:"\(%6\)";
Line 1,120: Line 1,176:
margin-left:1.5in;
margin-left:1.5in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l16:level7
+
@list l19:level7
{mso-level-tab-stop:1.75in;
{mso-level-tab-stop:1.75in;
mso-level-number-position:left;
mso-level-number-position:left;
margin-left:1.75in;
margin-left:1.75in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l16:level8
+
@list l19:level8
{mso-level-number-format:alpha-lower;
{mso-level-number-format:alpha-lower;
mso-level-tab-stop:2.0in;
mso-level-tab-stop:2.0in;
Line 1,131: Line 1,187:
margin-left:2.0in;
margin-left:2.0in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l16:level9
+
@list l19:level9
{mso-level-number-format:roman-lower;
{mso-level-number-format:roman-lower;
mso-level-tab-stop:2.25in;
mso-level-tab-stop:2.25in;
Line 1,137: Line 1,193:
margin-left:2.25in;
margin-left:2.25in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l17
+
@list l20
 +
{mso-list-id:1327439790;
 +
mso-list-type:hybrid;
 +
mso-list-template-ids:115351768 67698707 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
 +
@list l20:level1
 +
{mso-level-number-format:roman-upper;
 +
mso-level-tab-stop:none;
 +
mso-level-number-position:right;
 +
margin-left:.25in;
 +
text-indent:-.25in;}
 +
@list l21
{mso-list-id:1438332439;
{mso-list-id:1438332439;
mso-list-type:hybrid;
mso-list-type:hybrid;
mso-list-template-ids:2107017872 67698707 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
mso-list-template-ids:2107017872 67698707 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
-
@list l17:level1
+
@list l21:level1
{mso-level-number-format:roman-upper;
{mso-level-number-format:roman-upper;
mso-level-tab-stop:none;
mso-level-tab-stop:none;
Line 1,147: Line 1,213:
margin-left:1.0in;
margin-left:1.0in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l18
+
@list l22
{mso-list-id:1718239408;
{mso-list-id:1718239408;
mso-list-type:hybrid;
mso-list-type:hybrid;
mso-list-template-ids:-1271611110 67698715 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
mso-list-template-ids:-1271611110 67698715 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
-
@list l18:level1
+
@list l22:level1
{mso-level-number-format:roman-lower;
{mso-level-number-format:roman-lower;
mso-level-tab-stop:none;
mso-level-tab-stop:none;
mso-level-number-position:right;
mso-level-number-position:right;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l19
+
@list l23
{mso-list-id:1720205758;
{mso-list-id:1720205758;
mso-list-type:hybrid;
mso-list-type:hybrid;
mso-list-template-ids:-1195606094 67698713 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
mso-list-template-ids:-1195606094 67698713 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
-
@list l19:level1
+
@list l23:level1
{mso-level-number-format:alpha-lower;
{mso-level-number-format:alpha-lower;
mso-level-tab-stop:none;
mso-level-tab-stop:none;
Line 1,166: Line 1,232:
margin-left:1.25in;
margin-left:1.25in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l20
+
@list l24
{mso-list-id:1895461054;
{mso-list-id:1895461054;
mso-list-type:hybrid;
mso-list-type:hybrid;
mso-list-template-ids:2059586448 67698707 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
mso-list-template-ids:2059586448 67698707 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
-
@list l20:level1
+
@list l24:level1
{mso-level-number-format:roman-upper;
{mso-level-number-format:roman-upper;
mso-level-tab-stop:none;
mso-level-tab-stop:none;
Line 1,176: Line 1,242:
margin-left:.75in;
margin-left:.75in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l20:level2
+
@list l24:level2
{mso-level-number-format:alpha-lower;
{mso-level-number-format:alpha-lower;
mso-level-tab-stop:none;
mso-level-tab-stop:none;
Line 1,182: Line 1,248:
margin-left:1.25in;
margin-left:1.25in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l20:level3
+
@list l24:level3
{mso-level-number-format:roman-lower;
{mso-level-number-format:roman-lower;
mso-level-tab-stop:none;
mso-level-tab-stop:none;
Line 1,188: Line 1,254:
margin-left:1.75in;
margin-left:1.75in;
text-indent:-9.0pt;}
text-indent:-9.0pt;}
-
@list l21
+
@list l25
{mso-list-id:2097238860;
{mso-list-id:2097238860;
mso-list-template-ids:-266300996;}
mso-list-template-ids:-266300996;}
-
@list l21:level1
+
@list l25:level1
{mso-level-tab-stop:.25in;
{mso-level-tab-stop:.25in;
mso-level-number-position:left;
mso-level-number-position:left;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l21:level2
+
@list l25:level2
{mso-level-number-format:alpha-lower;
{mso-level-number-format:alpha-lower;
mso-level-tab-stop:.5in;
mso-level-tab-stop:.5in;
Line 1,201: Line 1,267:
margin-left:.75in;
margin-left:.75in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l21:level3
+
@list l25:level3
{mso-level-number-format:roman-lower;
{mso-level-number-format:roman-lower;
mso-level-tab-stop:.75in;
mso-level-tab-stop:.75in;
Line 1,207: Line 1,273:
margin-left:.1in;
margin-left:.1in;
text-indent:.65in;}
text-indent:.65in;}
-
@list l21:level4
+
@list l25:level4
{mso-level-text:"\(%4\)";
{mso-level-text:"\(%4\)";
mso-level-tab-stop:1.0in;
mso-level-tab-stop:1.0in;
Line 1,213: Line 1,279:
margin-left:1.0in;
margin-left:1.0in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l21:level5
+
@list l25:level5
{mso-level-number-format:alpha-lower;
{mso-level-number-format:alpha-lower;
mso-level-text:"\(%5\)";
mso-level-text:"\(%5\)";
Line 1,220: Line 1,286:
margin-left:1.25in;
margin-left:1.25in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l21:level6
+
@list l25:level6
{mso-level-number-format:roman-lower;
{mso-level-number-format:roman-lower;
mso-level-text:"\(%6\)";
mso-level-text:"\(%6\)";
Line 1,227: Line 1,293:
margin-left:1.5in;
margin-left:1.5in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l21:level7
+
@list l25:level7
{mso-level-tab-stop:1.75in;
{mso-level-tab-stop:1.75in;
mso-level-number-position:left;
mso-level-number-position:left;
margin-left:1.75in;
margin-left:1.75in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l21:level8
+
@list l25:level8
{mso-level-number-format:alpha-lower;
{mso-level-number-format:alpha-lower;
mso-level-tab-stop:2.0in;
mso-level-tab-stop:2.0in;
Line 1,238: Line 1,304:
margin-left:2.0in;
margin-left:2.0in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l21:level9
+
@list l25:level9
{mso-level-number-format:roman-lower;
{mso-level-number-format:roman-lower;
mso-level-tab-stop:2.25in;
mso-level-tab-stop:2.25in;
Line 1,244: Line 1,310:
margin-left:2.25in;
margin-left:2.25in;
text-indent:-.25in;}
text-indent:-.25in;}
-
@list l16:level2 lfo13
+
@list l26
 +
{mso-list-id:2119710673;
 +
mso-list-type:hybrid;
 +
mso-list-template-ids:-968488302 -2109568246 67698707 67698713 67698713 67698713 67698715 67698703 67698713 67698715;}
 +
@list l26:level1
 +
{mso-level-number-format:roman-upper;
 +
mso-level-tab-stop:none;
 +
mso-level-number-position:right;
 +
margin-left:1.25in;
 +
text-indent:-.25in;
 +
mso-ansi-font-style:normal;}
 +
@list l26:level2
 +
{mso-level-number-format:roman-upper;
 +
mso-level-tab-stop:none;
 +
mso-level-number-position:right;
 +
margin-left:1.25in;
 +
text-indent:-.25in;}
 +
@list l26:level3
 +
{mso-level-number-format:alpha-lower;
 +
mso-level-tab-stop:none;
 +
mso-level-number-position:left;
 +
text-indent:-9.0pt;}
 +
@list l26:level4
 +
{mso-level-number-format:alpha-lower;
 +
mso-level-tab-stop:none;
 +
mso-level-number-position:left;
 +
text-indent:-.25in;}
 +
@list l19:level2 lfo13
{mso-level-start-at:-1073762832;}
{mso-level-start-at:-1073762832;}
-
@list l16:level3 lfo13
+
@list l19:level3 lfo13
{mso-level-start-at:38108040;}
{mso-level-start-at:38108040;}
-
@list l16:level4 lfo13
+
@list l19:level4 lfo13
{mso-level-start-at:-1073762928;}
{mso-level-start-at:-1073762928;}
-
@list l16:level5 lfo13
+
@list l19:level5 lfo13
{mso-level-start-at:37978784;}
{mso-level-start-at:37978784;}
-
@list l16:level6 lfo13
+
@list l19:level6 lfo13
{mso-level-start-at:50823780;}
{mso-level-start-at:50823780;}
-
@list l16:level7 lfo13
+
@list l19:level7 lfo13
{mso-level-start-at:37970320;}
{mso-level-start-at:37970320;}
-
@list l16:level8 lfo13
+
@list l19:level8 lfo13
{mso-level-start-at:268374015;}
{mso-level-start-at:268374015;}
-
@list l16:level9 lfo13
+
@list l19:level9 lfo13
{mso-level-start-at:806748160;}
{mso-level-start-at:806748160;}
ol
ol
Line 1,264: Line 1,357:
ul
ul
{margin-bottom:0in;}
{margin-bottom:0in;}
 +
.style1 {color: #FFFFFF}
-->
-->
</style>
</style>
Line 1,305: Line 1,399:
   <o:idmap v:ext="edit" data="1"/>
   <o:idmap v:ext="edit" data="1"/>
  </o:shapelayout></xml><![endif]-->
  </o:shapelayout></xml><![endif]-->
-
 
</head>
</head>
Line 1,319: Line 1,412:
   background:#333333;mso-shading:windowtext;mso-pattern:gray-80 auto;
   background:#333333;mso-shading:windowtext;mso-pattern:gray-80 auto;
   padding:0in 5.4pt 0in 5.4pt'>
   padding:0in 5.4pt 0in 5.4pt'>
-
   <p class=MsoTitle style='margin-right:26.1pt'>Pipetting &amp; Making
+
   <p class=MsoTitle style1 style='margin-right:26.1pt'>Pipetting &amp; Making
-
   Solutions</p>
+
   Solutions</p> </td>
-
  </td>
+
-
 
+
   <td width=136 style='width:102.3pt;border:none;border-bottom:solid windowtext 2.25pt;
   <td width=136 style='width:102.3pt;border:none;border-bottom:solid windowtext 2.25pt;
   background:#333333;mso-shading:windowtext;mso-pattern:gray-80 auto;
   background:#333333;mso-shading:windowtext;mso-pattern:gray-80 auto;
   padding:0in 5.4pt 0in 5.4pt'>
   padding:0in 5.4pt 0in 5.4pt'>
-
   <p class=ExperimentNumber>Pg. 6</p>
+
   <p class=ExperimentNumber style1>Pg. 6</p> </td>
-
  </td>
+
  </tr>
  </tr>
</table>
</table>
<p class=MsoNormal style='margin-left:.75in;text-indent:-.75in;mso-text-indent-alt:
<p class=MsoNormal style='margin-left:.75in;text-indent:-.75in;mso-text-indent-alt:
-
-.25in;mso-list:l20 level1 lfo21'><![if !supportLists]><span
+
-.25in;mso-list:l24 level1 lfo21'><![if !supportLists]><span
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Line 1,340: Line 1,430:
style='color:windowtext;font-weight:normal;font-style:normal'>Pipetting<o:p></o:p></span></u></span></p>
style='color:windowtext;font-weight:normal;font-style:normal'>Pipetting<o:p></o:p></span></u></span></p>
-
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l20 level2 lfo21'><![if !supportLists]><span
+
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l24 level2 lfo21'><![if !supportLists]><span
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Line 1,348: Line 1,438:
pipettes properly<u><o:p></o:p></u></span></span></p>
pipettes properly<u><o:p></o:p></u></span></span></p>
-
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l20 level2 lfo21'><![if !supportLists]><span
+
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l24 level2 lfo21'><![if !supportLists]><span
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Line 1,356: Line 1,446:
down pipettes between solutions (with Ethanol)<u><o:p></o:p></u></span></span></p>
down pipettes between solutions (with Ethanol)<u><o:p></o:p></u></span></span></p>
-
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l20 level2 lfo21'><![if !supportLists]><span
+
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l24 level2 lfo21'><![if !supportLists]><span
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Line 1,365: Line 1,455:
<p class=MsoNormal style='margin-left:.75in;text-indent:-.75in;mso-text-indent-alt:
<p class=MsoNormal style='margin-left:.75in;text-indent:-.75in;mso-text-indent-alt:
-
-.25in;mso-list:l20 level1 lfo21'><![if !supportLists]><span
+
-.25in;mso-list:l24 level1 lfo21'><![if !supportLists]><span
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
style='mso-list:Ignore'><span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
style='mso-list:Ignore'><span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
</span>II.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
</span>II.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
-
 
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><u><span
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><u><span
style='color:windowtext;font-weight:normal;font-style:normal'>Solutions<o:p></o:p></span></u></span></p>
style='color:windowtext;font-weight:normal;font-style:normal'>Solutions<o:p></o:p></span></u></span></p>
-
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l20 level2 lfo21'><![if !supportLists]><span
+
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l24 level2 lfo21'><![if !supportLists]><span
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Line 1,383: Line 1,472:
<p class=MsoNormal style='margin-left:1.75in;text-indent:-1.75in;mso-text-indent-alt:
<p class=MsoNormal style='margin-left:1.75in;text-indent:-1.75in;mso-text-indent-alt:
-
-9.0pt;mso-list:l20 level3 lfo21'><![if !supportLists]><span
+
-9.0pt;mso-list:l24 level3 lfo21'><![if !supportLists]><span
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Line 1,392: Line 1,481:
<p class=MsoNormal style='margin-left:1.75in;text-indent:-1.75in;mso-text-indent-alt:
<p class=MsoNormal style='margin-left:1.75in;text-indent:-1.75in;mso-text-indent-alt:
-
-9.0pt;mso-list:l20 level3 lfo21'><![if !supportLists]><span
+
-9.0pt;mso-list:l24 level3 lfo21'><![if !supportLists]><span
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Line 1,401: Line 1,490:
<p class=MsoNormal style='margin-left:1.75in;text-indent:-1.75in;mso-text-indent-alt:
<p class=MsoNormal style='margin-left:1.75in;text-indent:-1.75in;mso-text-indent-alt:
-
-9.0pt;mso-list:l20 level3 lfo21'><![if !supportLists]><span
+
-9.0pt;mso-list:l24 level3 lfo21'><![if !supportLists]><span
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Line 1,419: Line 1,508:
   background:#333333;mso-shading:windowtext;mso-pattern:gray-80 auto;
   background:#333333;mso-shading:windowtext;mso-pattern:gray-80 auto;
   padding:0in 5.4pt 0in 5.4pt'>
   padding:0in 5.4pt 0in 5.4pt'>
-
   <p class=MsoTitle style='margin-right:26.1pt'>Plate Creation &amp; Cell
+
   <p class=MsoTitle style1 style='margin-right:26.1pt'>Plate Creation &amp; Cell
-
   Culture</p>
+
   Culture</p> </td>
-
 
+
-
  </td>
+
   <td width=136 style='width:102.3pt;border:none;border-bottom:solid windowtext 2.25pt;
   <td width=136 style='width:102.3pt;border:none;border-bottom:solid windowtext 2.25pt;
   background:#333333;mso-shading:windowtext;mso-pattern:gray-80 auto;
   background:#333333;mso-shading:windowtext;mso-pattern:gray-80 auto;
   padding:0in 5.4pt 0in 5.4pt'>
   padding:0in 5.4pt 0in 5.4pt'>
-
   <p class=ExperimentNumber>Pg. 7</p>
+
   <p class=ExperimentNumber style1>Pg. 7</p> </td>
-
  </td>
+
  </tr>
  </tr>
</table>
</table>
<p class=MsoNormal style='margin-left:.75in;text-indent:-.75in;mso-text-indent-alt:
<p class=MsoNormal style='margin-left:.75in;text-indent:-.75in;mso-text-indent-alt:
-
-.25in;mso-list:l14 level1 lfo23'><![if !supportLists]><span
+
-.25in;mso-list:l16 level1 lfo23'><![if !supportLists]><span
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Line 1,442: Line 1,528:
style='color:windowtext;font-weight:normal;font-style:normal'>:<u><o:p></o:p></u></span></span></p>
style='color:windowtext;font-weight:normal;font-style:normal'>:<u><o:p></o:p></u></span></span></p>
-
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l14 level2 lfo23'><![if !supportLists]><span
+
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l16 level2 lfo23'><![if !supportLists]><span
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Line 1,458: Line 1,544:
Bactoagar, cover with foil, autoclave for 20-30 minutes<o:p></o:p></span></span></p>
Bactoagar, cover with foil, autoclave for 20-30 minutes<o:p></o:p></span></span></p>
-
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l14 level2 lfo23'><![if !supportLists]><span
+
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l16 level2 lfo23'><![if !supportLists]><span
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Line 1,473: Line 1,559:
font-style:normal'><o:p></o:p></span></u></span></p>
font-style:normal'><o:p></o:p></span></u></span></p>
-
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l14 level2 lfo23'><![if !supportLists]><span
+
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l16 level2 lfo23'><![if !supportLists]><span
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Line 1,481: Line 1,567:
&amp; let swirl until dissolved<u><o:p></o:p></u></span></span></p>
&amp; let swirl until dissolved<u><o:p></o:p></u></span></span></p>
-
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l14 level2 lfo23'><![if !supportLists]><span
+
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l16 level2 lfo23'><![if !supportLists]><span
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Line 1,490: Line 1,576:
<p class=MsoNormal style='margin-left:.75in;text-indent:-.75in;mso-text-indent-alt:
<p class=MsoNormal style='margin-left:.75in;text-indent:-.75in;mso-text-indent-alt:
-
-.25in;mso-list:l14 level1 lfo23'><![if !supportLists]><span
+
-.25in;mso-list:l16 level1 lfo23'><![if !supportLists]><span
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Line 1,500: Line 1,586:
font-style:normal'>:<u><o:p></o:p></u></span></span></p>
font-style:normal'>:<u><o:p></o:p></u></span></span></p>
-
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l14 level2 lfo23'><![if !supportLists]><span
+
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l16 level2 lfo23'><![if !supportLists]><span
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Line 1,526: Line 1,612:
with cell density measurement (using the Spec)<o:p></o:p></span></span></p>
with cell density measurement (using the Spec)<o:p></o:p></span></span></p>
-
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l14 level2 lfo23'><![if !supportLists]><span
+
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l16 level2 lfo23'><![if !supportLists]><span
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Line 1,538: Line 1,624:
(for a miniprep); you will have to add 1mL, two times<o:p></o:p></span></span></p>
(for a miniprep); you will have to add 1mL, two times<o:p></o:p></span></span></p>
-
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l14 level2 lfo23'><![if !supportLists]><span
+
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l16 level2 lfo23'><![if !supportLists]><span
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Line 1,550: Line 1,636:
with your Initials, Sample Name (control or experimental) and the date<o:p></o:p></span></span></p>
with your Initials, Sample Name (control or experimental) and the date<o:p></o:p></span></span></p>
-
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l14 level2 lfo23'><![if !supportLists]><span
+
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l16 level2 lfo23'><![if !supportLists]><span
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Line 1,558: Line 1,644:
scraper in ethanol and flame lightly (2x) to sterilize<o:p></o:p></span></span></p>
scraper in ethanol and flame lightly (2x) to sterilize<o:p></o:p></span></span></p>
-
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l14 level2 lfo23'><![if !supportLists]><span
+
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l16 level2 lfo23'><![if !supportLists]><span
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Line 1,566: Line 1,652:
cool for a few seconds<o:p></o:p></span></span></p>
cool for a few seconds<o:p></o:p></span></span></p>
-
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l14 level2 lfo23'><![if !supportLists]><span
+
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l16 level2 lfo23'><![if !supportLists]><span
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Line 1,575: Line 1,661:
around to ensure the bacteria breaks off into the tube<o:p></o:p></span></span></p>
around to ensure the bacteria breaks off into the tube<o:p></o:p></span></span></p>
-
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l14 level2 lfo23'><![if !supportLists]><span
+
<p class=MsoNormal style='margin-left:1.25in;text-indent:-.25in;mso-list:l16 level2 lfo23'><![if !supportLists]><span
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
style='mso-list:Ignore'>g.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;
style='mso-list:Ignore'>g.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;
-
 
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
style='color:windowtext;font-weight:normal;font-style:normal'>Set incubator at
style='color:windowtext;font-weight:normal;font-style:normal'>Set incubator at
Line 1,586: Line 1,671:
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
font-style:normal'>C overnight and let shake gently for 12-14hrs<o:p></o:p></span></span></p>
font-style:normal'>C overnight and let shake gently for 12-14hrs<o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.25in'><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'><o:p>&nbsp;</o:p></span></span></p>
 +
 +
<table class=MsoNormalTable border=1 cellspacing=0 cellpadding=0
 +
style='margin-left:5.4pt;border-collapse:collapse;border:none;mso-border-bottom-alt:
 +
solid windowtext .5pt;mso-yfti-tbllook:191;mso-padding-alt:0in 5.4pt 0in 5.4pt'>
 +
<tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;mso-yfti-lastrow:yes'>
 +
  <td width=488 style='width:365.7pt;border:none;border-bottom:solid windowtext 2.25pt;
 +
  background:#333333;mso-shading:windowtext;mso-pattern:gray-80 auto;
 +
  padding:0in 5.4pt 0in 5.4pt'>
 +
  <p class=MsoTitle style1 style='margin-right:26.1pt'>Transformation of E.coli with
 +
  Recombinant DNA<o:p></o:p></p>  </td>
 +
  <td width=136 style='width:102.3pt;border:none;border-bottom:solid windowtext 2.25pt;
 +
  background:#333333;mso-shading:windowtext;mso-pattern:gray-80 auto;
 +
  padding:0in 5.4pt 0in 5.4pt'>
 +
  <p class=ExperimentNumber style1>Pg. 9<o:p></o:p></p>  </td>
 +
</tr>
 +
</table>
 +
 +
<p class=MsoNormal style='margin-left:.75in'><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>*note, </span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
mso-bidi-font-style:normal'>the heat shock</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
font-style:normal'> to the tubes is very important for transcription to occur<o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
font-weight:normal;font-style:normal'><span style='mso-tab-count:1'>            </span><u>+
 +
Controls</u>:<o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
font-weight:normal;font-style:normal'><span style='mso-tab-count:2'>                        </span>BBa_I13522
 +
: pTetGFP<o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
font-weight:normal;font-style:normal'><span style='mso-tab-count:2'>                        </span>BBa_J45220<o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
font-weight:normal;font-style:normal'><span style='mso-tab-count:1'>            </span><u>Group
 +
1:</u><o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
font-weight:normal;font-style:normal'><span style='mso-tab-count:2'>                        </span>BBa_R0011<o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
font-weight:normal;font-style:normal'><span style='mso-tab-count:2'>                        </span>BBa_J45219<o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
font-weight:normal;font-style:normal'><span style='mso-tab-count:1'>            </span><u>Group
 +
2:</u><o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
font-weight:normal;font-style:normal'><span style='mso-tab-count:2'>                        </span>BBa_I14032
 +
/ BBa_R0051 (same)<o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
font-weight:normal;font-style:normal'><span style='mso-tab-count:2'>                        </span>BBa_J06702<o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
font-weight:normal;font-style:normal'><span style='mso-tab-count:1'>            </span><u>Group
 +
3:</u><o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
font-weight:normal;font-style:normal'><span style='mso-tab-count:2'>                        </span>BBa_J04500<o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
font-weight:normal;font-style:normal'><span style='mso-tab-count:2'>                        </span>BBa_J04630
 +
/ BBa_I13401<o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
font-weight:normal;font-style:normal'><o:p>&nbsp;</o:p></span></span></p>
 +
 +
<p class=MsoNormal style='text-indent:.5in'><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;mso-bidi-font-style:normal'>Rima
 +
&amp; I did the transformation for Group 2:<o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.25in;text-indent:-1.25in;mso-text-indent-alt:
 +
-.25in;mso-list:l11 level1 lfo30'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
 +
style='mso-list:Ignore'><span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span>I.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>BBa_I14032</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
mso-bidi-font-style:normal'><o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.75in;text-indent:-.25in;mso-list:l11 level2 lfo30'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;mso-bidi-font-style:normal'><span
 +
style='mso-list:Ignore'>a.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;mso-bidi-font-style:normal'>Name: </span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
font-style:normal'>promoter P(Lac) IQ</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
mso-bidi-font-style:normal'><o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.75in;text-indent:-.25in;mso-list:l11 level2 lfo30'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;mso-bidi-font-style:normal'><span
 +
style='mso-list:Ignore'>b.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;mso-bidi-font-style:normal'>Sequence:
 +
</span></span><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
font-weight:normal;font-style:normal'>promoter</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
mso-bidi-font-style:normal'> </span></span><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>(-35, -10 bases
 +
before coding)</span></span><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;mso-bidi-font-style:normal'><o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.75in;text-indent:-.25in;mso-list:l11 level2 lfo30'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;mso-bidi-font-style:normal'><span
 +
style='mso-list:Ignore'>c.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;mso-bidi-font-style:normal'>Location</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
font-style:normal'>: 2007, Plate 2, Well 17K</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
mso-bidi-font-style:normal'><o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.75in;text-indent:-.25in;mso-list:l11 level2 lfo30'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;mso-bidi-font-style:normal'><span
 +
style='mso-list:Ignore'>d.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;mso-bidi-font-style:normal'>Plasmid:
 +
</span></span><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
font-weight:normal;font-style:normal'>pSB2K3</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
mso-bidi-font-style:normal'><o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.25in;text-indent:-1.25in;mso-text-indent-alt:
 +
-.25in;mso-list:l11 level1 lfo30'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
 +
style='mso-list:Ignore'><span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span>II.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>BBa_R0051</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
mso-bidi-font-style:normal'><o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.75in;text-indent:-.25in;mso-list:l11 level2 lfo30'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;mso-bidi-font-style:normal'><span
 +
style='mso-list:Ignore'>a.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;mso-bidi-font-style:normal'>Name:</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
font-style:normal'> promoter (lamda cl regulated)</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
mso-bidi-font-style:normal'><o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.75in;text-indent:-.25in;mso-list:l11 level2 lfo30'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;mso-bidi-font-style:normal'><span
 +
style='mso-list:Ignore'>b.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;mso-bidi-font-style:normal'>Sequence:</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
font-style:normal'> R0051 promoter</span></span><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;mso-bidi-font-style:normal'><o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.75in;text-indent:-.25in;mso-list:l11 level2 lfo30'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;mso-bidi-font-style:normal'><span
 +
style='mso-list:Ignore'>c.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;mso-bidi-font-style:normal'>Location:
 +
</span></span><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
font-weight:normal;font-style:normal'>2007, Plate 1, Well 9C</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
mso-bidi-font-style:normal'><o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.75in;text-indent:-.25in;mso-list:l11 level2 lfo30'><![if !supportLists]><i
 +
style='mso-bidi-font-style:normal'><span style='mso-fareast-font-family:Arial;
 +
mso-bidi-font-family:Arial'><span style='mso-list:Ignore'>d.<span
 +
style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp; </span></span></span></i><![endif]><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
mso-bidi-font-style:normal'>Plasmid:</span></span><i style='mso-bidi-font-style:
 +
normal'> </i>pSB1A2<i style='mso-bidi-font-style:normal'><o:p></o:p></i></p>
 +
 +
<p class=MsoNormal style='margin-left:1.25in;text-indent:-1.25in;mso-text-indent-alt:
 +
-.25in;mso-list:l11 level1 lfo30'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
 +
style='mso-list:Ignore'><span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span>III.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>BBa_J06702</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
mso-bidi-font-style:normal'><o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.75in;text-indent:-.25in;mso-list:l11 level2 lfo30'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;mso-bidi-font-style:normal'><span
 +
style='mso-list:Ignore'>a.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;mso-bidi-font-style:normal'>Name:</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
font-style:normal'> mCherry, bacterial with RBS &amp; forward terminator</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
mso-bidi-font-style:normal'><o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.75in;text-indent:-.25in;mso-list:l11 level2 lfo30'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;mso-bidi-font-style:normal'><span
 +
style='mso-list:Ignore'>b.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>Sequence</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
mso-bidi-font-style:normal'>: <o:p></o:p></span></span></p>
 +
 +
<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
 +
style='margin-left:159.5pt;border-collapse:collapse;border:none;mso-border-alt:
 +
solid windowtext .5pt;mso-yfti-tbllook:1184;mso-padding-alt:0in 5.4pt 0in 5.4pt'>
 +
<tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
 +
  <td width=70 valign=top style='width:52.8pt;border:solid windowtext 1.0pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0in 5.4pt 0in 5.4pt'>
 +
  <p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
  font-weight:normal;mso-bidi-font-style:normal'>RBS<o:p></o:p></span></span></p>
 +
  </td>
 +
  <td width=85 valign=top style='width:64.05pt;border:solid windowtext 1.0pt;
 +
  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
 +
  solid windowtext .5pt;padding:0in 5.4pt 0in 5.4pt'>
 +
  <p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
  font-weight:normal;mso-bidi-font-style:normal'>mCherry<o:p></o:p></span></span></p>
 +
  </td>
 +
  <td width=101 valign=top style='width:76.05pt;border:solid windowtext 1.0pt;
 +
  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
 +
  solid windowtext .5pt;padding:0in 5.4pt 0in 5.4pt'>
 +
  <p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
  font-weight:normal;mso-bidi-font-style:normal'>Terminator<o:p></o:p></span></span></p>
 +
  </td>
 +
  <td width=101 valign=top style='width:76.05pt;border:solid windowtext 1.0pt;
 +
  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
 +
  solid windowtext .5pt;padding:0in 5.4pt 0in 5.4pt'>
 +
  <p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
  font-weight:normal;mso-bidi-font-style:normal'>Terminator<o:p></o:p></span></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:1;mso-yfti-lastrow:yes'>
 +
  <td width=70 valign=top style='width:52.8pt;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0in 5.4pt 0in 5.4pt'>
 +
  <p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
  font-weight:normal;font-style:normal'>B0034<o:p></o:p></span></span></p>
 +
  </td>
 +
  <td width=85 valign=top style='width:64.05pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0in 5.4pt 0in 5.4pt'>
 +
  <p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
  font-weight:normal;font-style:normal'>J06504<o:p></o:p></span></span></p>
 +
  </td>
 +
  <td width=101 valign=top style='width:76.05pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0in 5.4pt 0in 5.4pt'>
 +
  <p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
  font-weight:normal;font-style:normal'>B0010<o:p></o:p></span></span></p>
 +
  </td>
 +
  <td width=101 valign=top style='width:76.05pt;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0in 5.4pt 0in 5.4pt'>
 +
  <p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
  font-weight:normal;font-style:normal'>B0012<o:p></o:p></span></span></p>
 +
  </td>
 +
</tr>
 +
</table>
 +
 +
<p class=MsoNormal style='margin-left:1.75in;text-indent:-.25in;mso-list:l11 level2 lfo30'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;mso-bidi-font-style:normal'><span
 +
style='mso-list:Ignore'>c.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;mso-bidi-font-style:normal'>Location:</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
font-style:normal'> 2007, Plate 2, Well 11D</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
mso-bidi-font-style:normal'><o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.75in;text-indent:-.25in;mso-list:l11 level2 lfo30'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;mso-bidi-font-style:normal'><span
 +
style='mso-list:Ignore'>d.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;mso-bidi-font-style:normal'>Plasmid:</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
font-style:normal'> pSB1A2</span></span><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;mso-bidi-font-style:normal'><o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.25in;text-indent:-1.25in;mso-text-indent-alt:
 +
-.25in;mso-list:l11 level1 lfo30'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
 +
style='mso-list:Ignore'><span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span>IV.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>Cells Used:
 +
E.coli: JM109 (Promega); Catalog #: L2001</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
mso-bidi-font-style:normal'><o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
font-weight:normal;mso-bidi-font-style:normal'><o:p>&nbsp;</o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.25in;text-indent:-1.25in;mso-text-indent-alt:
 +
-.25in;mso-list:l11 level1 lfo30'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
 +
style='mso-list:Ignore'><span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span>V.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>3 Plates Made:</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
mso-bidi-font-style:normal'><o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.75in;text-indent:-.25in;mso-list:l11 level2 lfo30'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;mso-bidi-font-style:normal'><span
 +
style='mso-list:Ignore'>a.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>Control: 100&#956;L
 +
LB / Cell</span></span><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
font-weight:normal;mso-bidi-font-style:normal'><o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.75in;text-indent:-.25in;mso-list:l11 level2 lfo30'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;mso-bidi-font-style:normal'><span
 +
style='mso-list:Ignore'>b.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>Test Plate 1: 100&#956;L
 +
LB/ Cell</span></span><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
font-weight:normal;mso-bidi-font-style:normal'><o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.75in;text-indent:-.25in;mso-list:l11 level2 lfo30'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;mso-bidi-font-style:normal'><span
 +
style='mso-list:Ignore'>c.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>Test Plate 2: 1&#956;L
 +
cell : 99&#956; H<sub>2</sub>O</span></span><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;mso-bidi-font-style:normal'><o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal><span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
font-weight:normal;font-style:normal'><o:p>&nbsp;</o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:.5in'><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;mso-bidi-font-style:normal'>Actual
 +
Transformation Procedure:</span></span><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'><o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.25in;text-indent:-1.25in;mso-text-indent-alt:
 +
-.25in;mso-list:l26 level1 lfo31'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
 +
style='mso-list:Ignore'><span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span>I.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>If using DNA from
 +
the registry, dilute with 9&#956;L of H<sub>2</sub>O. If using our own DNA, use
 +
200ng. <o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.5in;text-indent:-9.0pt;mso-list:l26 level3 lfo31'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
 +
style='mso-list:Ignore'>a.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>inject the water
 +
directly into the well, titerate, and then pipette it out<o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.25in;text-indent:-1.25in;mso-text-indent-alt:
 +
-.25in;mso-list:l26 level2 lfo31'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
 +
style='mso-list:Ignore'><span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span>II.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>Thaw 100&#956;L
 +
competent cells on ice. (50</span></span> <span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>&#956;L with our
 +
own/ 100</span></span> <span class=MsoIntenseEmphasis><span style='color:windowtext;
 +
font-weight:normal;font-style:normal'>&#956; if commercial cells used)<o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.25in;text-indent:-1.25in;mso-text-indent-alt:
 +
-.25in;mso-list:l26 level2 lfo31'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
 +
style='mso-list:Ignore'><span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span>III.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>Mix competent
 +
cells with 50</span></span> <span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>&#956; CaCl<sub>2</sub>
 +
(only if using our own cells)<o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.25in;text-indent:-1.25in;mso-text-indent-alt:
 +
-.25in;mso-list:l26 level2 lfo31'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
 +
style='mso-list:Ignore'><span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span>IV.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>Add 4</span></span>
 +
<span class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
font-style:normal'>&#956;L of registry DNA, or 200ng of our own DNA to the
 +
cells. <o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.25in;text-indent:-1.25in;mso-text-indent-alt:
 +
-.25in;mso-list:l26 level2 lfo31'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
 +
style='mso-list:Ignore'><span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span>V.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>Put in the ice
 +
bath for about 20 minutes. <o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.25in;text-indent:-1.25in;mso-text-indent-alt:
 +
-.25in;mso-list:l26 level2 lfo31'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
 +
style='mso-list:Ignore'><span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span>VI.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>Remove and
 +
immediately put in the hot water bath (42</span></span><span
 +
class=MsoIntenseEmphasis><span style='mso-bidi-font-family:Arial;color:windowtext;
 +
font-weight:normal;font-style:normal'>°</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
font-style:normal'>C) to heat shock for 45-60 sec.<o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.25in;text-indent:-1.25in;mso-text-indent-alt:
 +
-.25in;mso-list:l26 level2 lfo31'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
 +
style='mso-list:Ignore'><span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span>VII.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>Place back in the
 +
ice bath for 2 minutes. <o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.25in;text-indent:-1.25in;mso-text-indent-alt:
 +
-.25in;mso-list:l26 level2 lfo31'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
 +
style='mso-list:Ignore'><span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span>VIII.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>Add 900 &#956;L
 +
ice cold, antibiotic free LB to the cells<o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.25in;text-indent:-1.25in;mso-text-indent-alt:
 +
-.25in;mso-list:l26 level2 lfo31'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
 +
style='mso-list:Ignore'><span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span>IX.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>Incubate at 37</span></span><span
 +
class=MsoIntenseEmphasis><span style='mso-bidi-font-family:Arial;color:windowtext;
 +
font-weight:normal;font-style:normal'>°</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
font-style:normal'>C for 90 minutes in the shaking incubator.<o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.25in;text-indent:-1.25in;mso-text-indent-alt:
 +
-.25in;mso-list:l26 level2 lfo31'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
 +
style='mso-list:Ignore'><span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span>X.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>Dilute as desired,
 +
make the necessary controls, and plate cells using 100 &#956;L / plate. (200 if
 +
not commercial)<o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.25in;text-indent:-1.25in;mso-text-indent-alt:
 +
-.25in;mso-list:l26 level2 lfo31'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
 +
style='mso-list:Ignore'><span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span>XI.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>Incubate upside
 +
down (so that the label you wrote on it is face up) at 37</span></span><span
 +
class=MsoIntenseEmphasis><span style='mso-bidi-font-family:Arial;color:windowtext;
 +
font-weight:normal;font-style:normal'>°</span></span><span
 +
class=MsoIntenseEmphasis><span style='color:windowtext;font-weight:normal;
 +
font-style:normal'>C for 18-24 hours, then refrigerate. <o:p></o:p></span></span></p>
 +
 +
<p class=MsoNormal style='margin-left:1.5in;text-indent:-9.0pt;mso-list:l26 level3 lfo31'><![if !supportLists]><span
 +
class=MsoIntenseEmphasis><span style='mso-fareast-font-family:Arial;mso-bidi-font-family:
 +
Arial;color:windowtext;font-weight:normal;font-style:normal'><span
 +
style='mso-list:Ignore'>a.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span></span><![endif]><span class=MsoIntenseEmphasis><span
 +
style='color:windowtext;font-weight:normal;font-style:normal'>The controls
 +
should not have bacteria growing on them!<o:p></o:p></span></span></p>
</div>
</div>
Line 1,592: Line 2,180:
</html>
</html>
-
 
-
[[http://openwetware.org/wiki/User:Neil_J._Parikh return to my profile]]
 

Revision as of 19:51, 6 February 2008

This is an experiment title

Pipetting & Making Solutions

Pg. 6

              I.        Pipetting

a.    Learned to use pipettes properly

b.    Make sure to wipe down pipettes between solutions (with Ethanol)

c.    Change tips every time you go between solutions

            II.        Solutions

a.    Task: Make 0.3M KCl

                                                  i.    Salt (KCl): CAS 7447-40-7

                                                ii.    MW: 74.56g/mol

                                               iii.    2.2368 g / 100mL

 

Plate Creation & Cell Culture

Pg. 7

              I.        Steps for creating a plate:

a.    Dissolve 25g LB in 800mL of H2O

Bring the final volume to 1L in a 2L flask

Add 15g Bactoagar, cover with foil, autoclave for 20-30 minutes

b.    Bring back to lab, cool to 50°C while stirring

c.    Add100mg AMP & let swirl until dissolved

d.    Pour it into the plates (about 2-3mm deep)

            II.        Cell Culture:

a.    Use solution of LB with AMP

Ensure to keep the bags sealed to keep everything sterile

*Note once a bag of plates has been opened, the whole thing must be used

The tubes allow the solution to be aerated

The tubes with bacteria should look cloudy upon visual inspection—can be confirmed with cell density measurement (using the Spec)

b.    Sterilize the autopipette, set to 1mL (make a 2mL culture, however)

Add 2mL of the LB (for a miniprep); you will have to add 1mL, two times

c.    Label one as the control and another as your experimental sample

*Label the tubes with your Initials, Sample Name (control or experimental) and the date

d.    Dip the culture scraper in ethanol and flame lightly (2x) to sterilize

e.    Let the scraper cool for a few seconds

f.     Scrape off a tiny bit of bacteria off of the plate and place the scraper into the tube and shake around to ensure the bacteria breaks off into the tube

g.    Set incubator at 37°C overnight and let shake gently for 12-14hrs

 

Transformation of E.coli with Recombinant DNA

Pg. 9

*note, the heat shock to the tubes is very important for transcription to occur

+ Controls:

BBa_I13522 : pTetGFP

BBa_J45220

Group 1:

BBa_R0011

BBa_J45219

Group 2:

BBa_I14032 / BBa_R0051 (same)

BBa_J06702

Group 3:

BBa_J04500

BBa_J04630 / BBa_I13401

 

Rima & I did the transformation for Group 2:

                              I.        BBa_I14032

a.    Name: promoter P(Lac) IQ

b.    Sequence: promoter (-35, -10 bases before coding)

c.    Location: 2007, Plate 2, Well 17K

d.    Plasmid: pSB2K3

                            II.        BBa_R0051

a.    Name: promoter (lamda cl regulated)

b.    Sequence: R0051 promoter

c.    Location: 2007, Plate 1, Well 9C

d.    Plasmid: pSB1A2

                           III.        BBa_J06702

a.    Name: mCherry, bacterial with RBS & forward terminator

b.    Sequence:

RBS

mCherry

Terminator

Terminator

B0034

J06504

B0010

B0012

c.    Location: 2007, Plate 2, Well 11D

d.    Plasmid: pSB1A2

                          IV.        Cells Used: E.coli: JM109 (Promega); Catalog #: L2001

 

                           V.        3 Plates Made:

a.    Control: 100μL LB / Cell

b.    Test Plate 1: 100μL LB/ Cell

c.    Test Plate 2: 1μL cell : 99μ H2O

 

Actual Transformation Procedure:

                              I.        If using DNA from the registry, dilute with 9μL of H2O. If using our own DNA, use 200ng.

a.                inject the water directly into the well, titerate, and then pipette it out

                            II.        Thaw 100μL competent cells on ice. (50 μL with our own/ 100 μ if commercial cells used)

                           III.        Mix competent cells with 50 μ CaCl2 (only if using our own cells)

                          IV.        Add 4 μL of registry DNA, or 200ng of our own DNA to the cells.

                           V.        Put in the ice bath for about 20 minutes.

                          VI.        Remove and immediately put in the hot water bath (42°C) to heat shock for 45-60 sec.

                         VII.        Place back in the ice bath for 2 minutes.

                       VIII.        Add 900 μL ice cold, antibiotic free LB to the cells

                          IX.        Incubate at 37°C for 90 minutes in the shaking incubator.

                           X.        Dilute as desired, make the necessary controls, and plate cells using 100 μL / plate. (200 if not commercial)

                          XI.        Incubate upside down (so that the label you wrote on it is face up) at 37°C for 18-24 hours, then refrigerate.

a.                The controls should not have bacteria growing on them!

Personal tools