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| |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> |
| |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} |
| |- | | |- |
| | colspan="2"| | | | colspan="2"| |
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| The purpose of today's work is to: | | The purpose of today's work is to: |
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| # Make stock solutions of lysozyme and determine its molar absorptivity using the UV-Vis and Fluorescence Spectrophotometers | | # Make stock solutions of lysozyme |
| | # Measure fluorescence intensity and absorbance of lysozyme as a function of the concentration of lysozyme |
| | # Determine the molar absorptivity of lysozyme using the UV-Vis and Fluorescence Spectrophotometers |
| # Determine how protein concentration affects fluorescence intensity | | # Determine how protein concentration affects fluorescence intensity |
| # Prep a sample of our protease, alpha-chymotrypsin, for use in future experiments | | # Prep samples of our protease, alpha-chymotrypsin, for use in future experiments |
|
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| A full description of today's objectives and protocols are in Dr. Hartings's lab [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2015/09/09|notebook]]. | | A full description of today's objectives and protocols are in Dr. Hartings's lab [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2015/09/09|notebook]]. |
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| </ol> | | </ol> |
| </li> | | </li> |
| | | <li> Then, we prepped our stock samples of our protease, alpha-chymotrypsin, for use in future experiments. |
| | |
| <li> Then, we prepped our stock samples of our protease, alpha-chymotrypsin. | |
| <ol> | | <ol> |
| <li> First, we weighed the mass of the 1.5mL Eppindorf tube that we were going to use to store our stock of alpha-chymotrypsin. | | <li> First, we weighed the mass of the 1.5mL Eppindorf tube that we were going to use to store our stock of alpha-chymotrypsin. |
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| </li> | | </li> |
| </ol> | | </ol> |
| <li> After that, we took absorbance data for each of our lysozyme samples using UV-Vis Spectroscopy. | | <li> After that, we took absorbance and fluorescence data for each of our lysozyme samples. |
| </li> | | </li> |
| </ol> | | </ol> |
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| ==Data and Measurements== | | ==Data and Measurements== |
|
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| ===alpha-Chymotrpysin Standards===
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| <center>Table 1: Measurements for Each of the 15 alpha-Chymotrpysin Standards: Mass of Eppindorf Tube (g), Mass of alpha-Chymotrypsin (g), and Molarity of alpha-Chymotrypsin in 1mL HPLC Water (M)</center> | | <center>Table 1: Measurements for Each of the 15 alpha-Chymotrpysin Standards: Mass of Eppindorf Tube (g), Mass of alpha-Chymotrypsin (g), and Molarity of alpha-Chymotrypsin in 1mL HPLC Water (M)</center> |
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| | 1.02781 || 0.00121 || 47.265625 | | | 1.02781 || 0.00121 || 47.265625 |
| |} | | |} |
| </center>
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|
| |
| ===Fluorescence===
| |
|
| |
| <center>Table 3: Raw Fluorescence Data for Blank (HPLC Water) and Lysozyme Standards for Wavelengths 300-500nm</center>
| |
|
| |
|
| |
| <center>
| |
|
| |
| {| class="wikitable" border="1"
| |
| ! Wavelength (nm) !! Fluorescence of Blank (HPLC Water) (0M) !! Fluorescence of 0.965M Standard !! Fluorescence of 1.93M Standard !! Fluorescence of 3.86M Standard !! Fluorescence of 7.225M Standard !! Fluorescence of 15.445M Standard
| |
| |-
| |
| | 300.00 || 0.241000 || 1.714000 || 4.044000 || 7.223000 || 10.010000 || 14.053000
| |
| |-
| |
| | 300.50 || 0.231798 || 2.033250 || 4.319538 || 7.736019 || 11.304191 || 15.803222
| |
| |-
| |
| | 301.00 || 0.225261 || 2.338888 || 4.614535 || 8.285435 || 12.590322 || 17.547442
| |
| |-
| |
| | 301.50 || 0.223047 || 2.635071 || 4.949361 || 8.903301 || 13.889913 || 19.315293
| |
| |-
| |
| | 302.00 || 0.228552 || 2.924992 || 5.323778 || 9.592161 || 15.225661 || 21.129509
| |
| |-
| |
| | 302.50 || 0.244522 || 3.212784 || 5.739772 || 10.375192 || 16.603248 || 23.008914
| |
| |-
| |
| | 303.00 || 0.267249 || 3.498136 || 6.213206 || 11.250088 || 18.029729 || 24.981096
| |
| |-
| |
| | 303.50 || 0.295490 || 3.782348 || 6.743837 || 12.221671 || 19.515792 || 27.078760
| |
| |-
| |
| | 304.00 || 0.330513 || 4.080415 || 7.320252 || 13.279723 || 21.111722 || 29.280986
| |
| |-
| |
| | 304.50 || 0.372482 || 4.407780 || 7.943491 || 14.416857 || 22.836812 || 31.628813
| |
| |-
| |
| | 305.00 || 0.419023 || 4.760684 || 8.607499 || 15.626171 || 24.688707 || 34.156151
| |
| |-
| |
| | 305.50 || 0.471697 || 5.145484 || 9.310102 || 16.909374 || 26.640423 || 36.849717
| |
| |-
| |
| | 306.00 || 0.528381 || 5.561600 || 10.049318 || 18.272295 || 28.692961 || 39.692439
| |
| |-
| |
| | 306.50 || 0.588148 || 6.018216 || 10.819654 || 19.712849 || 30.882519 || 42.721680
| |
| |-
| |
| | 307.00 || 0.651476 || 6.509498 || 11.627992 || 21.225987 || 33.172505 || 45.961683
| |
| |-
| |
| | 307.50 || 0.715064 || 7.028985 || 12.486288 || 22.815255 || 35.581102 || 49.374521
| |
| |-
| |
| | 308.00 || 0.777629 || 7.568474 || 13.405930 || 24.481414 || 38.077647 || 52.984636
| |
| |-
| |
| | 308.50 || 0.841886 || 8.136667 || 14.385542 || 26.221722 || 40.687618 || 56.748090
| |
| |-
| |
| | 309.00 || 0.910814 || 8.733959 || 15.436765 || 28.039348 || 43.432797 || 60.633703
| |
| |-
| |
| | 309.50 || 0.979747 || 9.348815 || 16.551743 || 29.930526 || 46.336476 || 64.700986
| |
| |-
| |
| | 310.00 || 1.052985 || 9.993592 || 17.723677 || 31.897695 || 49.355132 || 68.941426
| |
| |-
| |
| | 310.50 || 1.128943 || 10.657105 || 18.951345 || 33.931165 || 52.486879 || 73.304546
| |
| |-
| |
| | 311.00 || 1.203638 || 11.353547 || 20.218586 || 36.037246 || 55.762869 || 77.841888
| |
| |-
| |
| | 311.50 || 1.275243 || 12.081265 || 21.516561 || 38.248507 || 59.123397 || 82.575548
| |
| |-
| |
| | 312.00 || 1.341320 || 12.833839 || 22.839916 || 40.548694 || 62.639971 || 87.489119
| |
| |-
| |
| | 312.50 || 1.401930 || 13.600307 || 24.199256 || 42.925690 || 66.286749 || 92.622357
| |
| |-
| |
| | 313.00 || 1.457897 || 14.378439 || 25.590860 || 45.378449 || 70.040078 || 97.957017
| |
| |-
| |
| | 313.50 || 1.509168 || 15.166029 || 27.030541 || 47.930261 || 73.937197 || 103.471745
| |
| |-
| |
| | 314.00 || 1.550367 || 15.956243 || 28.514118 || 50.578133 || 77.983355 || 109.193128
| |
| |-
| |
| | 314.50 || 1.588591 || 16.761309 || 30.036566 || 53.278026 || 82.149906 || 115.083112
| |
| |-
| |
| | 315.00 || 1.622839 || 17.561858 || 31.604185 || 56.004031 || 86.473877 || 121.074565
| |
| |-
| |
| | 315.50 || 1.653910 || 18.368003 || 33.199677 || 58.741792 || 90.905967 || 127.150642
| |
| |-
| |
| | 316.00 || 1.676645 || 19.189059 || 34.802280 || 61.546736 || 95.373750 || 133.292232
| |
| |-
| |
| | 316.50 || 1.688100 || 20.027096 || 36.411613 || 64.416710 || 99.913260 || 139.454796
| |
| |-
| |
| | 317.00 || 1.687316 || 20.874330 || 38.033591 || 67.332325 || 104.517087 || 145.680434
| |
| |-
| |
| | 317.50 || 1.677851 || 21.723087 || 39.664651 || 70.268426 || 109.123967 || 152.002493
| |
| |-
| |
| | 318.00 || 1.659588 || 22.578469 || 41.302072 || 73.241000 || 113.809483 || 158.365797
| |
| |-
| |
| | 318.50 || 1.627299 || 23.447834 || 42.957462 || 76.282643 || 118.490301 || 164.873876
| |
| |-
| |
| | 319.00 || 1.588279 || 24.344866 || 44.643633 || 79.370905 || 123.136889 || 171.524240
| |
| |-
| |
| | 319.50 || 1.540120 || 25.262456 || 46.395009 || 82.476069 || 127.886727 || 178.311649
| |
| |-
| |
| | 320.00 || 1.487784 || 26.172040 || 48.197345 || 85.580486 || 132.708429 || 185.261878
| |
| |-
| |
| | 320.50 || 1.431515 || 27.061038 || 50.031867 || 88.739111 || 137.537459 || 192.141519
| |
| |-
| |
| | 321.00 || 1.370547 || 27.943139 || 51.899806 || 91.962865 || 142.371398 || 198.965641
| |
| |-
| |
| | 321.50 || 1.303949 || 28.825355 || 53.791944 || 95.240965 || 147.229535 || 205.774010
| |
| |-
| |
| | 322.00 || 1.234534 || 29.679596 || 55.680727 || 98.555997 || 152.071789 || 212.529652
| |
| |-
| |
| | 322.50 || 1.163718 || 30.506110 || 57.551065 || 101.807071 || 156.967597 || 219.002715
| |
| |-
| |
| | 323.00 || 1.088592 || 31.321452 || 59.407955 || 105.029333 || 161.811408 || 225.306517
| |
| |-
| |
| | 323.50 || 1.013374 || 32.143539 || 61.250589 || 108.216203 || 166.503724 || 231.494407
| |
| |-
| |
| | 324.00 || 0.935516 || 32.998722 || 63.092338 || 111.295935 || 171.187025 || 237.596126
| |
| |-
| |
| | 324.50 || 0.858543 || 33.878885 || 64.932341 || 114.326070 || 175.905241 || 243.747562
| |
| |-
| |
| | 325.00 || 0.786717 || 34.756982 || 66.764450 || 117.288911 || 180.566628 || 249.734639
| |
| |-
| |
| | 325.50 || 0.721899 || 35.649098 || 68.604459 || 120.226812 || 185.109834 || 255.653893
| |
| |-
| |
| | 326.00 || 0.662493 || 36.549759 || 70.440135 || 123.169980 || 189.636505 || 261.613026
| |
| |-
| |
| | 326.50 || 0.608481 || 37.438986 || 72.235667 || 126.187625 || 194.113282 || 267.782319
| |
| |-
| |
| | 327.00 || 0.561378 || 38.302427 || 74.017799 || 129.204503 || 198.611000 || 274.018876
| |
| |-
| |
| | 327.50 || 0.517635 || 39.144049 || 75.808014 || 132.178958 || 203.068188 || 280.325295
| |
| |-
| |
| | 328.00 || 0.477328 || 39.964845 || 77.564340 || 135.168428 || 207.480860 || 286.816561
| |
| |-
| |
| | 328.50 || 0.440311 || 40.776506 || 79.294141 || 138.126234 || 211.943094 || 293.341866
| |
| |-
| |
| | 329.00 || 0.404451 || 41.594963 || 81.013649 || 141.124212 || 216.595542 || 299.951529
| |
| |-
| |
| | 329.50 || 0.371818 || 42.406246 || 82.745713 || 144.111031 || 221.309561 || 306.357598
| |
| |-
| |
| | 330.00 || 0.342575 || 43.232720 || 84.498626 || 147.109101 || 226.001771 || 312.608981
| |
| |-
| |
| | 330.50 || 0.319803 || 44.090254 || 86.289314 || 150.123907 || 230.832136 || 318.755678
| |
| |-
| |
| | 331.00 || 0.303472 || 44.960347 || 88.068401 || 153.234060 || 235.721319 || 324.806829
| |
| |-
| |
| | 331.50 || 0.293388 || 45.823830 || 89.821319 || 156.409063 || 240.594550 || 330.797695
| |
| |-
| |
| | 332.00 || 0.287203 || 46.697960 || 91.662854 || 159.600202 || 245.414284 || 336.841457
| |
| |-
| |
| | 332.50 || 0.284443 || 47.590948 || 93.539375 || 162.791756 || 250.124610 || 343.163714
| |
| |-
| |
| | 333.00 || 0.286100 || 48.474547 || 95.430130 || 166.006709 || 254.822204 || 349.589072
| |
| |-
| |
| | 333.50 || 0.289189 || 49.358627 || 97.374829 || 169.251131 || 259.475905 || 356.050526
| |
| |-
| |
| | 334.00 || 0.294262 || 50.238124 || 99.360932 || 172.453367 || 264.062773 || 362.402779
| |
| |-
| |
| | 334.50 || 0.299687 || 51.146806 || 101.300915 || 175.646262 || 268.557047 || 368.767402
| |
| |-
| |
| | 335.00 || 0.305888 || 52.111671 || 103.223620 || 178.825965 || 273.210854 || 375.109536
| |
| |-
| |
| | 335.50 || 0.312726 || 53.100454 || 105.115814 || 182.021157 || 277.988825 || 381.305389
| |
| |-
| |
| | 336.00 || 0.319610 || 54.077822 || 106.865303 || 185.197714 || 282.788945 || 387.333443
| |
| |-
| |
| | 336.50 || 0.325969 || 55.037293 || 108.554984 || 188.357622 || 287.487920 || 393.102570
| |
| |-
| |
| | 337.00 || 0.332133 || 56.011370 || 110.201862 || 191.453135 || 291.977576 || 398.906062
| |
| |-
| |
| | 337.50 || 0.339752 || 56.964508 || 111.773394 || 194.609056 || 296.403765 || 404.642546
| |
| |-
| |
| | 338.00 || 0.347187 || 57.869486 || 113.370228 || 197.705086 || 300.590277 || 410.224634
| |
| |-
| |
| | 338.50 || 0.354729 || 58.690616 || 115.023242 || 200.708693 || 304.552556 || 415.441628
| |
| |-
| |
| | 339.00 || 0.361840 || 59.443958 || 116.547721 || 203.653150 || 308.181091 || 420.628580
| |
| |-
| |
| | 339.50 || 0.368037 || 60.163504 || 117.998814 || 206.501435 || 311.780847 || 425.822416
| |
| |-
| |
| | 340.00 || 0.371995 || 60.876663 || 119.458452 || 209.392859 || 315.513079 || 431.044814
| |
| |-
| |
| | 340.50 || 0.372619 || 61.566413 || 120.825722 || 212.187387 || 319.430157 || 436.140747
| |
| |-
| |
| | 341.00 || 0.371161 || 62.216235 || 122.107321 || 214.874762 || 323.502902 || 440.959416
| |
| |-
| |
| | 341.50 || 0.367424 || 62.875154 || 123.405788 || 217.354468 || 327.505169 || 445.873114
| |
| |-
| |
| | 342.00 || 0.364801 || 63.550169 || 124.643201 || 219.769151 || 331.511910 || 450.680387
| |
| |-
| |
| | 342.50 || 0.362544 || 64.237528 || 125.909961 || 221.924271 || 335.321738 || 455.339873
| |
| |-
| |
| | 343.00 || 0.360998 || 64.906196 || 127.292411 || 223.877914 || 338.931730 || 459.526271
| |
| |-
| |
| | 343.50 || 0.363582 || 65.556329 || 128.581088 || 225.622618 || 342.080522 || 463.356394
| |
| |-
| |
| | 344.00 || 0.369850 || 66.167929 || 129.739049 || 227.117762 || 344.891935 || 466.899503
| |
| |-
| |
| | 344.50 || 0.375878 || 66.778819 || 130.786469 || 228.687038 || 347.366814 || 470.273955
| |
| |-
| |
| | 345.00 || 0.379619 || 67.369149 || 131.727633 || 230.301144 || 349.778372 || 473.429169
| |
| |-
| |
| | 345.50 || 0.381009 || 67.921596 || 132.537467 || 231.955967 || 352.321561 || 476.218581
| |
| |-
| |
| | 346.00 || 0.380781 || 68.435974 || 133.301394 || 233.612248 || 354.715990 || 479.139436
| |
| |-
| |
| | 346.50 || 0.379489 || 68.901450 || 133.984232 || 235.391374 || 356.932624 || 481.922963
| |
| |-
| |
| | 347.00 || 0.376442 || 69.351444 || 134.599693 || 237.089954 || 358.935173 || 484.714909
| |
| |-
| |
| | 347.50 || 0.371793 || 69.747640 || 135.316938 || 238.727553 || 360.831438 || 487.529915
| |
| |-
| |
| | 348.00 || 0.368520 || 70.119142 || 135.985622 || 240.084952 || 362.398125 || 490.253073
| |
| |-
| |
| | 348.50 || 0.368683 || 70.437333 || 136.609629 || 240.995634 || 363.823477 || 492.791884
| |
| |-
| |
| | 349.00 || 0.370428 || 70.719375 || 137.147764 || 241.700033 || 365.036545 || 495.176924
| |
| |-
| |
| | 349.50 || 0.371883 || 70.977578 || 137.597510 || 242.263049 || 366.107423 || 497.182066
| |
| |-
| |
| | 350.00 || 0.371002 || 71.256414 || 137.976468 || 242.744036 || 367.323661 || 498.774158
| |
| |-
| |
| | 350.50 || 0.371108 || 71.546095 || 138.361586 || 243.129993 || 368.667440 || 500.322668
| |
| |-
| |
| | 351.00 || 0.372381 || 71.807204 || 138.760988 || 243.547647 || 370.058620 || 501.523618
| |
| |-
| |
| | 351.50 || 0.372891 || 72.069842 || 139.068831 || 243.943684 || 371.438373 || 502.522668
| |
| |-
| |
| | 352.00 || 0.372046 || 72.261051 || 139.424944 || 244.531851 || 372.602579 || 503.634323
| |
| |-
| |
| | 352.50 || 0.371036 || 72.409496 || 139.719329 || 245.129163 || 373.370207 || 504.868428
| |
| |-
| |
| | 353.00 || 0.371942 || 72.530773 || 139.988722 || 245.536615 || 373.868403 || 505.766006
| |
| |-
| |
| | 353.50 || 0.372674 || 72.588649 || 140.177425 || 245.947373 || 374.190401 || 506.402384
| |
| |-
| |
| | 354.00 || 0.373601 || 72.594528 || 140.238144 || 246.329610 || 374.302935 || 506.660380
| |
| |-
| |
| | 354.50 || 0.372428 || 72.612724 || 140.189735 || 246.579962 || 374.196043 || 506.589061
| |
| |-
| |
| | 355.00 || 0.370898 || 72.653629 || 140.080610 || 246.661435 || 374.042081 || 506.208645
| |
| |-
| |
| | 355.50 || 0.369204 || 72.671974 || 139.936786 || 246.532481 || 374.032310 || 505.391271
| |
| |-
| |
| | 356.00 || 0.367906 || 72.707963 || 139.670159 || 246.149481 || 374.239922 || 504.233793
| |
| |-
| |
| | 356.50 || 0.367337 || 72.694224 || 139.422947 || 245.689515 || 374.500411 || 503.039327
| |
| |-
| |
| | 357.00 || 0.368067 || 72.651944 || 139.141360 || 245.178133 || 374.597356 || 502.177858
| |
| |-
| |
| | 357.50 || 0.370435 || 72.617637 || 138.864367 || 244.496761 || 374.438652 || 501.343360
| |
| |-
| |
| | 358.00 || 0.372765 || 72.529968 || 138.616267 || 243.856885 || 374.288043 || 500.742477
| |
| |-
| |
| | 358.50 || 0.374523 || 72.394248 || 138.384012 || 243.327259 || 373.976753 || 500.405178
| |
| |-
| |
| | 359.00 || 0.375919 || 72.213766 || 138.103666 || 242.951969 || 373.303745 || 500.270283
| |
| |-
| |
| | 359.50 || 0.375539 || 72.006829 || 137.827440 || 242.765472 || 372.314333 || 499.991812
| |
| |-
| |
| | 360.00 || 0.372407 || 71.743635 || 137.517548 || 242.649965 || 371.120216 || 499.235268
| |
| |-
| |
| | 360.50 || 0.367333 || 71.458239 || 137.079302 || 242.390576 || 369.740284 || 498.040691
| |
| |-
| |
| | 361.00 || 0.361116 || 71.136387 || 136.561813 || 241.965253 || 368.168357 || 496.457534
| |
| |-
| |
| | 361.50 || 0.354592 || 70.752255 || 135.970655 || 241.361921 || 366.545328 || 494.664697
| |
| |-
| |
| | 362.00 || 0.348977 || 70.365007 || 135.240119 || 240.438008 || 364.854178 || 492.590181
| |
| |-
| |
| | 362.50 || 0.344424 || 69.970161 || 134.374969 || 239.356345 || 363.265015 || 490.416984
| |
| |-
| |
| | 363.00 || 0.340666 || 69.609714 || 133.457689 || 238.090117 || 361.655524 || 488.433700
| |
| |-
| |
| | 363.50 || 0.338177 || 69.249191 || 132.455915 || 236.647176 || 360.004180 || 486.419915
| |
| |-
| |
| | 364.00 || 0.337989 || 68.887772 || 131.481903 || 235.197234 || 358.417684 || 484.321056
| |
| |-
| |
| | 364.50 || 0.338189 || 68.523873 || 130.492829 || 233.722744 || 356.881066 || 481.959303
| |
| |-
| |
| | 365.00 || 0.337824 || 68.168923 || 129.446480 || 232.162369 || 355.173649 || 479.425932
| |
| |-
| |
| | 365.50 || 0.336966 || 67.821551 || 128.424359 || 230.545175 || 353.256018 || 476.732871
| |
| |-
| |
| | 366.00 || 0.335171 || 67.417953 || 127.458805 || 228.887191 || 351.165441 || 473.759583
| |
| |-
| |
| | 366.50 || 0.330991 || 66.981353 || 126.450223 || 227.253824 || 348.964178 || 470.520962
| |
| |-
| |
| | 367.00 || 0.327906 || 66.511565 || 125.405391 || 225.734219 || 346.649057 || 467.146052
| |
| |-
| |
| | 367.50 || 0.323983 || 66.044464 || 124.439364 || 224.209755 || 344.114227 || 463.980857
| |
| |-
| |
| | 368.00 || 0.318844 || 65.531352 || 123.498353 || 222.606442 || 341.288940 || 460.755660
| |
| |-
| |
| | 368.50 || 0.313968 || 64.927241 || 122.639059 || 220.956033 || 338.366612 || 457.610265
| |
| |-
| |
| | 369.00 || 0.310341 || 64.291334 || 121.778764 || 219.249169 || 335.417469 || 454.297527
| |
| |-
| |
| | 369.50 || 0.307127 || 63.650484 || 120.810948 || 217.437411 || 332.439232 || 450.883458
| |
| |-
| |
| | 370.00 || 0.305673 || 63.018059 || 119.795361 || 215.520228 || 329.255405 || 447.338686
| |
| |-
| |
| | 370.50 || 0.305727 || 62.382573 || 118.746984 || 213.398910 || 325.946620 || 443.705327
| |
| |-
| |
| | 371.00 || 0.304421 || 61.755015 || 117.540609 || 211.145544 || 322.695302 || 439.893452
| |
| |-
| |
| | 371.50 || 0.304052 || 61.157028 || 116.217675 || 208.873845 || 319.479842 || 435.725337
| |
| |-
| |
| | 372.00 || 0.304116 || 60.599396 || 114.833849 || 206.628029 || 316.374089 || 431.531200
| |
| |-
| |
| | 372.50 || 0.304467 || 60.056198 || 113.411744 || 204.327994 || 313.310297 || 427.234798
| |
| |-
| |
| | 373.00 || 0.303769 || 59.462235 || 112.087699 || 202.002618 || 310.358090 || 423.002866
| |
| |-
| |
| | 373.50 || 0.302520 || 58.856185 || 110.827144 || 199.568918 || 307.453498 || 418.723526
| |
| |-
| |
| | 374.00 || 0.297634 || 58.255279 || 109.581462 || 197.102967 || 304.600177 || 414.447378
| |
| |-
| |
| | 374.50 || 0.291205 || 57.636931 || 108.382178 || 194.708998 || 301.625682 || 410.199402
| |
| |-
| |
| | 375.00 || 0.285347 || 57.003770 || 107.252474 || 192.304304 || 298.475716 || 406.078814
| |
| |-
| |
| | 375.50 || 0.278794 || 56.359559 || 106.088506 || 189.969037 || 295.193339 || 401.982251
| |
| |-
| |
| | 376.00 || 0.272180 || 55.720465 || 104.946968 || 187.673467 || 291.696288 || 397.660687
| |
| |-
| |
| | 376.50 || 0.265485 || 55.071710 || 103.796226 || 185.478384 || 288.016449 || 393.077310
| |
| |-
| |
| | 377.00 || 0.262183 || 54.411590 || 102.585262 || 183.380467 || 284.249778 || 388.351688
| |
| |-
| |
| | 377.50 || 0.260435 || 53.708887 || 101.376171 || 181.334440 || 280.536841 || 383.649589
| |
| |-
| |
| | 378.00 || 0.262169 || 52.983156 || 100.133835 || 179.145404 || 277.020333 || 378.977142
| |
| |-
| |
| | 378.50 || 0.264448 || 52.252562 || 98.851584 || 176.821647 || 273.704128 || 374.382293
| |
| |-
| |
| | 379.00 || 0.267181 || 51.504223 || 97.478286 || 174.484893 || 270.406154 || 369.724865
| |
| |-
| |
| | 379.50 || 0.269149 || 50.740045 || 96.069811 || 172.087723 || 267.082602 || 365.055355
| |
| |-
| |
| | 380.00 || 0.270462 || 49.978099 || 94.578658 || 169.687675 || 263.759763 || 360.399288
| |
| |-
| |
| | 380.50 || 0.270031 || 49.226474 || 93.129213 || 167.266442 || 260.463435 || 355.653277
| |
| |-
| |
| | 381.00 || 0.266458 || 48.480120 || 91.729232 || 164.833208 || 257.041097 || 350.784672
| |
| |-
| |
| | 381.50 || 0.263157 || 47.765471 || 90.299282 || 162.403081 || 253.389590 || 345.843386
| |
| |-
| |
| | 382.00 || 0.258768 || 47.072566 || 88.884398 || 160.042590 || 249.732638 || 340.975457
| |
| |-
| |
| | 382.50 || 0.255837 || 46.403884 || 87.481811 || 157.701096 || 246.198457 || 336.175521
| |
| |-
| |
| | 383.00 || 0.254182 || 45.750378 || 86.108782 || 155.351809 || 242.677824 || 331.563202
| |
| |-
| |
| | 383.50 || 0.252973 || 45.101318 || 84.712538 || 153.026722 || 239.092339 || 327.087722
| |
| |-
| |
| | 384.00 || 0.250253 || 44.431684 || 83.315220 || 150.715633 || 235.442058 || 322.539007
| |
| |-
| |
| | 384.50 || 0.248277 || 43.768957 || 81.891341 || 148.439666 || 231.701246 || 317.988866
| |
| |-
| |
| | 385.00 || 0.246910 || 43.099270 || 80.478005 || 146.188523 || 227.968630 || 313.363977
| |
| |-
| |
| | 385.50 || 0.244153 || 42.413973 || 79.118049 || 143.931574 || 224.247860 || 308.523632
| |
| |-
| |
| | 386.00 || 0.241519 || 41.740293 || 77.757755 || 141.650135 || 220.394324 || 303.476167
| |
| |-
| |
| | 386.50 || 0.236890 || 41.055341 || 76.403205 || 139.315775 || 216.616045 || 298.318515
| |
| |-
| |
| | 387.00 || 0.231405 || 40.366621 || 75.076608 || 136.973689 || 212.997750 || 293.005039
| |
| |-
| |
| | 387.50 || 0.226671 || 39.669535 || 73.743325 || 134.655454 || 209.418753 || 287.805393
| |
| |-
| |
| | 388.00 || 0.223350 || 38.967123 || 72.395336 || 132.352143 || 205.863544 || 282.782461
| |
| |-
| |
| | 388.50 || 0.219483 || 38.243141 || 71.045884 || 130.052117 || 202.384722 || 277.779360
| |
| |-
| |
| | 389.00 || 0.217998 || 37.529258 || 69.695807 || 127.737637 || 198.816116 || 273.108206
| |
| |-
| |
| | 389.50 || 0.218427 || 36.815574 || 68.368203 || 125.452514 || 195.203036 || 268.695623
| |
| |-
| |
| | 390.00 || 0.220571 || 36.105243 || 67.093268 || 123.189117 || 191.575678 || 264.398296
| |
| |-
| |
| | 390.50 || 0.222586 || 35.426485 || 65.812875 || 120.898743 || 187.830616 || 260.111126
| |
| |-
| |
| | 391.00 || 0.223659 || 34.762338 || 64.543854 || 118.527936 || 184.244223 || 255.881855
| |
| |-
| |
| | 391.50 || 0.224835 || 34.112049 || 63.317009 || 116.134316 || 180.843646 || 251.492780
| |
| |-
| |
| | 392.00 || 0.224717 || 33.474376 || 62.095950 || 113.761771 || 177.433872 || 246.894615
| |
| |-
| |
| | 392.50 || 0.222958 || 32.825863 || 60.893196 || 111.424517 || 174.003033 || 242.130613
| |
| |-
| |
| | 393.00 || 0.218389 || 32.154175 || 59.669424 || 109.124536 || 170.669733 || 237.153112
| |
| |-
| |
| | 393.50 || 0.212999 || 31.492301 || 58.442356 || 106.841398 || 167.381846 || 232.233238
| |
| |-
| |
| | 394.00 || 0.207047 || 30.846229 || 57.222987 || 104.599246 || 164.052805 || 227.433151
| |
| |-
| |
| | 394.50 || 0.203065 || 30.226801 || 56.015538 || 102.434746 || 160.623064 || 222.703512
| |
| |-
| |
| | 395.00 || 0.199762 || 29.649779 || 54.817187 || 100.369301 || 157.103313 || 218.040627
| |
| |-
| |
| | 395.50 || 0.197459 || 29.089115 || 53.633864 || 98.298842 || 153.635306 || 213.546854
| |
| |-
| |
| | 396.00 || 0.196459 || 28.532345 || 52.498001 || 96.235283 || 150.334190 || 209.205808
| |
| |-
| |
| | 396.50 || 0.196023 || 27.994742 || 51.399292 || 94.194325 || 147.056785 || 204.908050
| |
| |-
| |
| | 397.00 || 0.196498 || 27.449236 || 50.357355 || 92.209361 || 143.836460 || 200.580185
| |
| |-
| |
| | 397.50 || 0.197307 || 26.884843 || 49.346300 || 90.283420 || 140.709034 || 196.295610
| |
| |-
| |
| | 398.00 || 0.196956 || 26.312121 || 48.358625 || 88.373970 || 137.741340 || 192.127899
| |
| |-
| |
| | 398.50 || 0.195823 || 25.748297 || 47.439516 || 86.439994 || 134.923101 || 188.081331
| |
| |-
| |
| | 399.00 || 0.194707 || 25.188177 || 46.549303 || 84.534368 || 132.149877 || 184.200456
| |
| |-
| |
| | 399.50 || 0.192423 || 24.640907 || 45.665220 || 82.738630 || 129.431356 || 180.355962
| |
| |-
| |
| | 400.00 || 0.189316 || 24.096797 || 44.771287 || 80.970929 || 126.773396 || 176.560737
| |
| |-
| |
| | 400.50 || 0.186599 || 23.545813 || 43.863217 || 79.204778 || 124.221422 || 172.858115
| |
| |-
| |
| | 401.00 || 0.184316 || 23.020603 || 42.955787 || 77.450780 || 121.672897 || 169.104028
| |
| |-
| |
| | 401.50 || 0.182411 || 22.504951 || 42.050291 || 75.712045 || 119.151797 || 165.290148
| |
| |-
| |
| | 402.00 || 0.181768 || 22.012362 || 41.138748 || 74.020459 || 116.612083 || 161.460604
| |
| |-
| |
| | 402.50 || 0.180402 || 21.536381 || 40.240854 || 72.418893 || 114.087767 || 157.707917
| |
| |-
| |
| | 403.00 || 0.179345 || 21.094426 || 39.403568 || 70.813907 || 111.583046 || 154.058622
| |
| |-
| |
| | 403.50 || 0.177252 || 20.666260 || 38.601570 || 69.212147 || 109.088928 || 150.541162
| |
| |-
| |
| | 404.00 || 0.174663 || 20.250654 || 37.840699 || 67.688586 || 106.655867 || 147.220218
| |
| |-
| |
| | 404.50 || 0.171977 || 19.858701 || 37.109062 || 66.234614 || 104.230159 || 144.069668
| |
| |-
| |
| | 405.00 || 0.171130 || 19.466078 || 36.376302 || 64.825616 || 101.865222 || 141.089498
| |
| |-
| |
| | 405.50 || 0.173460 || 19.064962 || 35.650744 || 63.447415 || 99.541425 || 138.189650
| |
| |-
| |
| | 406.00 || 0.177682 || 18.650175 || 34.923349 || 62.073825 || 97.318363 || 135.327353
| |
| |-
| |
| | 406.50 || 0.183255 || 18.245150 || 34.171119 || 60.711990 || 95.170130 || 132.434043
| |
| |-
| |
| | 407.00 || 0.187496 || 17.847904 || 33.401384 || 59.435838 || 93.129455 || 129.570836
| |
| |-
| |
| | 407.50 || 0.191804 || 17.473492 || 32.652787 || 58.202646 || 91.118543 || 126.744589
| |
| |-
| |
| | 408.00 || 0.195016 || 17.107265 || 31.934982 || 56.994123 || 89.151944 || 123.947292
| |
| |-
| |
| | 408.50 || 0.195631 || 16.729286 || 31.256532 || 55.793332 || 87.228437 || 121.239778
| |
| |-
| |
| | 409.00 || 0.191518 || 16.360066 || 30.626373 || 54.615815 || 85.334891 || 118.593798
| |
| |-
| |
| | 409.50 || 0.185078 || 15.997840 || 30.020604 || 53.432390 || 83.433362 || 116.023039
| |
| |-
| |
| | 410.00 || 0.179706 || 15.628447 || 29.438228 || 52.258675 || 81.535592 || 113.535999
| |
| |-
| |
| | 410.50 || 0.175366 || 15.259245 || 28.888567 || 51.080549 || 79.696892 || 111.148232
| |
| |-
| |
| | 411.00 || 0.172105 || 14.902929 || 28.349795 || 49.896812 || 77.937829 || 108.770454
| |
| |-
| |
| | 411.50 || 0.168445 || 14.564308 || 27.812320 || 48.754308 || 76.289728 || 106.422331
| |
| |-
| |
| | 412.00 || 0.165334 || 14.255232 || 27.271793 || 47.669186 || 74.708178 || 104.118023
| |
| |-
| |
| | 412.50 || 0.164049 || 13.971103 || 26.722184 || 46.653661 || 73.194188 || 101.865033
| |
| |-
| |
| | 413.00 || 0.164538 || 13.695407 || 26.183196 || 45.681651 || 71.748312 || 99.701741
| |
| |-
| |
| | 413.50 || 0.165184 || 13.434946 || 25.663104 || 44.754542 || 70.361803 || 97.568765
| |
| |-
| |
| | 414.00 || 0.165336 || 13.185642 || 25.161909 || 43.858005 || 68.981331 || 95.481597
| |
| |-
| |
| | 414.50 || 0.165487 || 12.925281 || 24.660132 || 42.986793 || 67.601360 || 93.449223
| |
| |-
| |
| | 415.00 || 0.167828 || 12.664712 || 24.180629 || 42.127732 || 66.228490 || 91.507290
| |
| |-
| |
| | 415.50 || 0.172016 || 12.405018 || 23.702129 || 41.268732 || 64.878670 || 89.620730
| |
| |-
| |
| | 416.00 || 0.175422 || 12.140917 || 23.213281 || 40.418137 || 63.565060 || 87.789835
| |
| |-
| |
| | 416.50 || 0.176980 || 11.878674 || 22.713632 || 39.598778 || 62.287078 || 85.931737
| |
| |-
| |
| | 417.00 || 0.177415 || 11.624665 || 22.206993 || 38.819464 || 61.039118 || 84.061463
| |
| |-
| |
| | 417.50 || 0.178109 || 11.386554 || 21.705441 || 38.069377 || 59.811265 || 82.262399
| |
| |-
| |
| | 418.00 || 0.178402 || 11.157434 || 21.229602 || 37.340542 || 58.606049 || 80.492202
| |
| |-
| |
| | 418.50 || 0.177909 || 10.937803 || 20.787258 || 36.636956 || 57.409007 || 78.782260
| |
| |-
| |
| | 419.00 || 0.175863 || 10.717470 || 20.363463 || 35.946715 || 56.229015 || 77.137324
| |
| |-
| |
| | 419.50 || 0.174925 || 10.504650 || 19.986810 || 35.265869 || 55.051142 || 75.579723
| |
| |-
| |
| | 420.00 || 0.174812 || 10.301324 || 19.641235 || 34.580581 || 53.872817 || 74.108908
| |
| |-
| |
| | 420.50 || 0.174587 || 10.099629 || 19.317321 || 33.876414 || 52.696074 || 72.755096
| |
| |-
| |
| | 421.00 || 0.174148 || 9.903409 || 18.997837 || 33.184547 || 51.549209 || 71.411554
| |
| |-
| |
| | 421.50 || 0.173708 || 9.708992 || 18.675010 || 32.502227 || 50.430512 || 70.049901
| |
| |-
| |
| | 422.00 || 0.173154 || 9.526338 || 18.331536 || 31.832656 || 49.371601 || 68.725587
| |
| |-
| |
| | 422.50 || 0.172392 || 9.352436 || 17.993481 || 31.164844 || 48.356484 || 67.369206
| |
| |-
| |
| | 423.00 || 0.171820 || 9.182520 || 17.648667 || 30.541309 || 47.415917 || 66.005104
| |
| |-
| |
| | 423.50 || 0.170172 || 9.013173 || 17.284303 || 29.942965 || 46.533804 || 64.665762
| |
| |-
| |
| | 424.00 || 0.169549 || 8.843318 || 16.927955 || 29.361083 || 45.702902 || 63.345275
| |
| |-
| |
| | 424.50 || 0.170360 || 8.677997 || 16.582517 || 28.784729 || 44.888227 || 62.050667
| |
| |-
| |
| | 425.00 || 0.170847 || 8.513688 || 16.268087 || 28.209086 || 44.060041 || 60.830328
| |
| |-
| |
| | 425.50 || 0.170816 || 8.339209 || 15.964781 || 27.645122 || 43.231479 || 59.607369
| |
| |-
| |
| | 426.00 || 0.171668 || 8.154962 || 15.685908 || 27.067421 || 42.381384 || 58.353932
| |
| |-
| |
| | 426.50 || 0.172651 || 7.970920 || 15.412178 || 26.479839 || 41.511123 || 57.126302
| |
| |-
| |
| | 427.00 || 0.173323 || 7.789397 || 15.151645 || 25.870978 || 40.602914 || 55.884612
| |
| |-
| |
| | 427.50 || 0.174311 || 7.608142 || 14.886372 || 25.272218 || 39.723169 || 54.627824
| |
| |-
| |
| | 428.00 || 0.174887 || 7.432996 || 14.605185 || 24.687255 || 38.854920 || 53.401155
| |
| |-
| |
| | 428.50 || 0.175054 || 7.265122 || 14.302670 || 24.120456 || 38.047280 || 52.219371
| |
| |-
| |
| | 429.00 || 0.175656 || 7.112031 || 13.985699 || 23.586157 || 37.299851 || 51.098958
| |
| |-
| |
| | 429.50 || 0.177150 || 6.974077 || 13.675852 || 23.099039 || 36.577697 || 50.042858
| |
| |-
| |
| | 430.00 || 0.177351 || 6.842703 || 13.360342 || 22.656178 || 35.890505 || 49.010677
| |
| |-
| |
| | 430.50 || 0.177094 || 6.711117 || 13.075755 || 22.241117 || 35.223810 || 48.001706
| |
| |-
| |
| | 431.00 || 0.175629 || 6.580747 || 12.817567 || 21.846350 || 34.559536 || 47.016892
| |
| |-
| |
| | 431.50 || 0.173414 || 6.453718 || 12.588411 || 21.455313 || 33.852156 || 46.049806
| |
| |-
| |
| | 432.00 || 0.171822 || 6.318818 || 12.377115 || 21.061444 || 33.148545 || 45.091477
| |
| |-
| |
| | 432.50 || 0.169375 || 6.186656 || 12.176981 || 20.646844 || 32.418703 || 44.146473
| |
| |-
| |
| | 433.00 || 0.164802 || 6.049459 || 11.981965 || 20.193678 || 31.663193 || 43.201908
| |
| |-
| |
| | 433.50 || 0.161387 || 5.911410 || 11.788373 || 19.718482 || 30.905302 || 42.280136
| |
| |-
| |
| | 434.00 || 0.161389 || 5.776325 || 11.589969 || 19.247283 || 30.137213 || 41.401626
| |
| |-
| |
| | 434.50 || 0.162519 || 5.643817 || 11.368645 || 18.774048 || 29.391067 || 40.541084
| |
| |-
| |
| | 435.00 || 0.164148 || 5.513529 || 11.143736 || 18.314649 || 28.657586 || 39.685713
| |
| |-
| |
| | 435.50 || 0.165175 || 5.388900 || 10.915283 || 17.875959 || 27.958942 || 38.834500
| |
| |-
| |
| | 436.00 || 0.165270 || 5.276248 || 10.684513 || 17.459220 || 27.273699 || 37.994794
| |
| |-
| |
| | 436.50 || 0.166147 || 5.162618 || 10.452425 || 17.072779 || 26.630305 || 37.164407
| |
| |-
| |
| | 437.00 || 0.164594 || 5.056164 || 10.220302 || 16.705272 || 26.032074 || 36.351175
| |
| |-
| |
| | 437.50 || 0.159410 || 4.945837 || 10.001300 || 16.337873 || 25.457377 || 35.528382
| |
| |-
| |
| | 438.00 || 0.152608 || 4.833787 || 9.807576 || 15.972132 || 24.900638 || 34.712296
| |
| |-
| |
| | 438.50 || 0.146839 || 4.716925 || 9.629898 || 15.612556 || 24.344158 || 33.925934
| |
| |-
| |
| | 439.00 || 0.142506 || 4.595642 || 9.453358 || 15.252622 || 23.826065 || 33.144917
| |
| |-
| |
| | 439.50 || 0.139671 || 4.473986 || 9.281508 || 14.902666 || 23.300272 || 32.370478
| |
| |-
| |
| | 440.00 || 0.138100 || 4.351024 || 9.110176 || 14.558570 || 22.781194 || 31.602403
| |
| |-
| |
| | 440.50 || 0.137776 || 4.241174 || 8.939498 || 14.213080 || 22.250418 || 30.846251
| |
| |-
| |
| | 441.00 || 0.141383 || 4.140192 || 8.765150 || 13.872654 || 21.722439 || 30.093888
| |
| |-
| |
| | 441.50 || 0.144747 || 4.052866 || 8.583542 || 13.545550 || 21.213479 || 29.386829
| |
| |-
| |
| | 442.00 || 0.146561 || 3.971137 || 8.397596 || 13.234213 || 20.706386 || 28.701244
| |
| |-
| |
| | 442.50 || 0.147074 || 3.897082 || 8.216496 || 12.932719 || 20.211069 || 28.043524
| |
| |-
| |
| | 443.00 || 0.146444 || 3.827473 || 8.042445 || 12.640304 || 19.711102 || 27.409700
| |
| |-
| |
| | 443.50 || 0.144614 || 3.757734 || 7.872784 || 12.349784 || 19.253562 || 26.800544
| |
| |-
| |
| | 444.00 || 0.143231 || 3.690782 || 7.703542 || 12.067866 || 18.809233 || 26.211553
| |
| |-
| |
| | 444.50 || 0.142458 || 3.618186 || 7.536042 || 11.803482 || 18.377561 || 25.639666
| |
| |-
| |
| | 445.00 || 0.141322 || 3.542795 || 7.371767 || 11.546465 || 17.963831 || 25.063644
| |
| |-
| |
| | 445.50 || 0.142596 || 3.467263 || 7.206978 || 11.289391 || 17.571151 || 24.459655
| |
| |-
| |
| | 446.00 || 0.142871 || 3.396635 || 7.052337 || 11.034301 || 17.202805 || 23.875094
| |
| |-
| |
| | 446.50 || 0.143760 || 3.321843 || 6.902722 || 10.786674 || 16.838203 || 23.306554
| |
| |-
| |
| | 447.00 || 0.144535 || 3.244584 || 6.760773 || 10.552477 || 16.479590 || 22.775281
| |
| |-
| |
| | 447.50 || 0.144050 || 3.169252 || 6.628384 || 10.327175 || 16.124527 || 22.261473
| |
| |-
| |
| | 448.00 || 0.140058 || 3.097379 || 6.507249 || 10.113661 || 15.786481 || 21.774905
| |
| |-
| |
| | 448.50 || 0.134928 || 3.030638 || 6.390064 || 9.902634 || 15.452978 || 21.317341
| |
| |-
| |
| | 449.00 || 0.130107 || 2.963436 || 6.272898 || 9.699410 || 15.107347 || 20.899345
| |
| |-
| |
| | 449.50 || 0.125690 || 2.895199 || 6.153528 || 9.500765 || 14.769273 || 20.496651
| |
| |-
| |
| | 450.00 || 0.123620 || 2.829324 || 6.027215 || 9.299178 || 14.448781 || 20.080969
| |
| |-
| |
| | 450.50 || 0.121650 || 2.766576 || 5.902107 || 9.093951 || 14.143862 || 19.663784
| |
| |-
| |
| | 451.00 || 0.121358 || 2.702926 || 5.776199 || 8.879343 || 13.839795 || 19.235970
| |
| |-
| |
| | 451.50 || 0.123710 || 2.637002 || 5.651252 || 8.662433 || 13.536698 || 18.805089
| |
| |-
| |
| | 452.00 || 0.127726 || 2.572945 || 5.528463 || 8.448570 || 13.241660 || 18.373074
| |
| |-
| |
| | 452.50 || 0.129180 || 2.511183 || 5.413388 || 8.244975 || 12.946885 || 17.956958
| |
| |-
| |
| | 453.00 || 0.128621 || 2.455453 || 5.306440 || 8.044594 || 12.653587 || 17.548163
| |
| |-
| |
| | 453.50 || 0.127061 || 2.404509 || 5.213040 || 7.858088 || 12.356118 || 17.170490
| |
| |-
| |
| | 454.00 || 0.124396 || 2.353308 || 5.119316 || 7.688640 || 12.066343 || 16.807409
| |
| |-
| |
| | 454.50 || 0.120768 || 2.302635 || 5.025524 || 7.538431 || 11.796886 || 16.457915
| |
| |-
| |
| | 455.00 || 0.115057 || 2.256183 || 4.932731 || 7.403724 || 11.538076 || 16.127911
| |
| |-
| |
| | 455.50 || 0.109699 || 2.212005 || 4.839600 || 7.272688 || 11.294062 || 15.798616
| |
| |-
| |
| | 456.00 || 0.106269 || 2.165590 || 4.747361 || 7.147958 || 11.069739 || 15.461571
| |
| |-
| |
| | 456.50 || 0.104884 || 2.119696 || 4.656631 || 7.021148 || 10.863505 || 15.119931
| |
| |-
| |
| | 457.00 || 0.104608 || 2.072950 || 4.564393 || 6.892557 || 10.655410 || 14.792868
| |
| |-
| |
| | 457.50 || 0.105958 || 2.025725 || 4.471174 || 6.750106 || 10.443017 || 14.469215
| |
| |-
| |
| | 458.00 || 0.108230 || 1.979651 || 4.392610 || 6.598314 || 10.225838 || 14.156946
| |
| |-
| |
| | 458.50 || 0.111584 || 1.932622 || 4.315147 || 6.440129 || 10.012332 || 13.849794
| |
| |-
| |
| | 459.00 || 0.112509 || 1.888250 || 4.238628 || 6.287152 || 9.803061 || 13.548789
| |
| |-
| |
| | 459.50 || 0.110564 || 1.848319 || 4.165503 || 6.139908 || 9.594397 || 13.266492
| |
| |-
| |
| | 460.00 || 0.107337 || 1.812232 || 4.095596 || 5.998609 || 9.382381 || 12.989650
| |
| |-
| |
| | 460.50 || 0.103632 || 1.780220 || 4.030624 || 5.871743 || 9.185847 || 12.718943
| |
| |-
| |
| | 461.00 || 0.098064 || 1.753873 || 3.973367 || 5.762399 || 9.005024 || 12.448622
| |
| |-
| |
| | 461.50 || 0.091398 || 1.729105 || 3.916768 || 5.664781 || 8.826698 || 12.189978
| |
| |-
| |
| | 462.00 || 0.086238 || 1.703874 || 3.852803 || 5.566949 || 8.649068 || 11.951732
| |
| |-
| |
| | 462.50 || 0.083283 || 1.679229 || 3.792756 || 5.471155 || 8.474225 || 11.727490
| |
| |-
| |
| | 463.00 || 0.084834 || 1.650765 || 3.725643 || 5.369943 || 8.305142 || 11.511942
| |
| |-
| |
| | 463.50 || 0.087382 || 1.620276 || 3.652324 || 5.266337 || 8.137822 || 11.302113
| |
| |-
| |
| | 464.00 || 0.089726 || 1.587388 || 3.574561 || 5.159191 || 7.969741 || 11.099427
| |
| |-
| |
| | 464.50 || 0.092708 || 1.550840 || 3.497780 || 5.049099 || 7.800986 || 10.897618
| |
| |-
| |
| | 465.00 || 0.095336 || 1.512421 || 3.425784 || 4.941380 || 7.642719 || 10.692595
| |
| |-
| |
| | 465.50 || 0.095976 || 1.473782 || 3.365278 || 4.839634 || 7.490625 || 10.483267
| |
| |-
| |
| | 466.00 || 0.093990 || 1.439567 || 3.316317 || 4.745137 || 7.346521 || 10.264016
| |
| |-
| |
| | 466.50 || 0.090498 || 1.411064 || 3.273919 || 4.647761 || 7.220312 || 10.057934
| |
| |-
| |
| | 467.00 || 0.086796 || 1.388671 || 3.232453 || 4.557805 || 7.103315 || 9.859264
| |
| |-
| |
| | 467.50 || 0.084668 || 1.368226 || 3.189030 || 4.475139 || 6.996225 || 9.675906
| |
| |-
| |
| | 468.00 || 0.083650 || 1.353595 || 3.147103 || 4.396376 || 6.890356 || 9.513829
| |
| |-
| |
| | 468.50 || 0.083877 || 1.341457 || 3.100533 || 4.314938 || 6.785614 || 9.369235
| |
| |-
| |
| | 469.00 || 0.085215 || 1.327460 || 3.055874 || 4.238876 || 6.680655 || 9.244495
| |
| |-
| |
| | 469.50 || 0.086624 || 1.310635 || 3.012067 || 4.168760 || 6.581571 || 9.123980
| |
| |-
| |
| | 470.00 || 0.087442 || 1.286096 || 2.972592 || 4.102112 || 6.477173 || 9.005117
| |
| |-
| |
| | 470.50 || 0.087902 || 1.261538 || 2.939849 || 4.044315 || 6.369455 || 8.867348
| |
| |-
| |
| | 471.00 || 0.086836 || 1.237685 || 2.911629 || 3.982841 || 6.265521 || 8.726090
| |
| |-
| |
| | 471.50 || 0.084699 || 1.212456 || 2.876779 || 3.922584 || 6.163749 || 8.576797
| |
| |-
| |
| | 472.00 || 0.082035 || 1.187069 || 2.831494 || 3.867193 || 6.063035 || 8.424534
| |
| |-
| |
| | 472.50 || 0.079202 || 1.165741 || 2.787914 || 3.812161 || 5.961652 || 8.282925
| |
| |-
| |
| | 473.00 || 0.077538 || 1.149507 || 2.740362 || 3.750914 || 5.856902 || 8.143670
| |
| |-
| |
| | 473.50 || 0.076996 || 1.135963 || 2.691465 || 3.689712 || 5.753216 || 8.014836
| |
| |-
| |
| | 474.00 || 0.077467 || 1.124832 || 2.643794 || 3.626629 || 5.658951 || 7.893250
| |
| |-
| |
| | 474.50 || 0.077394 || 1.109002 || 2.601289 || 3.562375 || 5.566747 || 7.781103
| |
| |-
| |
| | 475.00 || 0.078557 || 1.094735 || 2.571581 || 3.503290 || 5.474199 || 7.667822
| |
| |-
| |
| | 475.50 || 0.081072 || 1.081535 || 2.551920 || 3.444979 || 5.382296 || 7.550396
| |
| |-
| |
| | 476.00 || 0.082832 || 1.065496 || 2.531475 || 3.388122 || 5.301017 || 7.422898
| |
| |-
| |
| | 476.50 || 0.083098 || 1.046970 || 2.504803 || 3.335241 || 5.227389 || 7.302013
| |
| |-
| |
| | 477.00 || 0.082523 || 1.027164 || 2.480674 || 3.291482 || 5.155138 || 7.198735
| |
| |-
| |
| | 477.50 || 0.082591 || 1.007323 || 2.455450 || 3.252002 || 5.085266 || 7.099115
| |
| |-
| |
| | 478.00 || 0.081909 || 0.989143 || 2.429680 || 3.215858 || 5.018255 || 7.001949
| |
| |-
| |
| | 478.50 || 0.080900 || 0.973354 || 2.404356 || 3.176445 || 4.958068 || 6.916981
| |
| |-
| |
| | 479.00 || 0.079080 || 0.957897 || 2.379455 || 3.135794 || 4.907095 || 6.839887
| |
| |-
| |
| | 479.50 || 0.076659 || 0.945676 || 2.364848 || 3.093705 || 4.858003 || 6.767306
| |
| |-
| |
| | 480.00 || 0.076272 || 0.935497 || 2.355429 || 3.050350 || 4.803622 || 6.694711
| |
| |-
| |
| | 480.50 || 0.074426 || 0.926002 || 2.345201 || 3.007163 || 4.757615 || 6.612657
| |
| |-
| |
| | 481.00 || 0.072125 || 0.918301 || 2.330621 || 2.963062 || 4.713423 || 6.530074
| |
| |-
| |
| | 481.50 || 0.069559 || 0.911399 || 2.312179 || 2.924292 || 4.664414 || 6.450526
| |
| |-
| |
| | 482.00 || 0.067773 || 0.904471 || 2.289224 || 2.895079 || 4.606850 || 6.373684
| |
| |-
| |
| | 482.50 || 0.065922 || 0.896640 || 2.263807 || 2.872859 || 4.543268 || 6.299136
| |
| |-
| |
| | 483.00 || 0.064724 || 0.886670 || 2.234906 || 2.850302 || 4.473668 || 6.229722
| |
| |-
| |
| | 483.50 || 0.064889 || 0.876728 || 2.200258 || 2.829106 || 4.408878 || 6.158192
| |
| |-
| |
| | 484.00 || 0.063948 || 0.867439 || 2.169798 || 2.805534 || 4.344413 || 6.086715
| |
| |-
| |
| | 484.50 || 0.064583 || 0.854850 || 2.145287 || 2.779804 || 4.277265 || 6.014202
| |
| |-
| |
| | 485.00 || 0.064009 || 0.840840 || 2.121537 || 2.752895 || 4.218881 || 5.937835
| |
| |-
| |
| | 485.50 || 0.064643 || 0.825958 || 2.103259 || 2.721192 || 4.168304 || 5.873851
| |
| |-
| |
| | 486.00 || 0.066108 || 0.811122 || 2.086251 || 2.687226 || 4.128233 || 5.813181
| |
| |-
| |
| | 486.50 || 0.068161 || 0.798821 || 2.070989 || 2.656340 || 4.092333 || 5.757264
| |
| |-
| |
| | 487.00 || 0.070126 || 0.786967 || 2.058376 || 2.629232 || 4.061081 || 5.711160
| |
| |-
| |
| | 487.50 || 0.071301 || 0.775375 || 2.045972 || 2.604352 || 4.032446 || 5.671963
| |
| |-
| |
| | 488.00 || 0.072878 || 0.766198 || 2.029892 || 2.581274 || 4.008126 || 5.632790
| |
| |-
| |
| | 488.50 || 0.072788 || 0.760072 || 2.012953 || 2.555965 || 3.980830 || 5.590720
| |
| |-
| |
| | 489.00 || 0.072334 || 0.755532 || 1.999528 || 2.531326 || 3.954601 || 5.535734
| |
| |-
| |
| | 489.50 || 0.068607 || 0.751542 || 1.981887 || 2.510179 || 3.923733 || 5.468444
| |
| |-
| |
| | 490.00 || 0.066046 || 0.747299 || 1.964610 || 2.489841 || 3.884455 || 5.405712
| |
| |-
| |
| | 490.50 || 0.065106 || 0.742297 || 1.943104 || 2.469419 || 3.843859 || 5.340091
| |
| |-
| |
| | 491.00 || 0.065232 || 0.740020 || 1.924001 || 2.449360 || 3.796521 || 5.271697
| |
| |-
| |
| | 491.50 || 0.065791 || 0.735078 || 1.909306 || 2.431854 || 3.745246 || 5.215323
| |
| |-
| |
| | 492.00 || 0.068121 || 0.727868 || 1.900010 || 2.417185 || 3.697327 || 5.170391
| |
| |-
| |
| | 492.50 || 0.071863 || 0.720770 || 1.892082 || 2.402916 || 3.653334 || 5.132563
| |
| |-
| |
| | 493.00 || 0.075672 || 0.712789 || 1.885303 || 2.379989 || 3.611121 || 5.103104
| |
| |-
| |
| | 493.50 || 0.079394 || 0.704316 || 1.881637 || 2.349144 || 3.584452 || 5.069952
| |
| |-
| |
| | 494.00 || 0.080268 || 0.695391 || 1.874674 || 2.316365 || 3.564069 || 5.022875
| |
| |-
| |
| | 494.50 || 0.079475 || 0.686384 || 1.864988 || 2.283875 || 3.545234 || 4.971731
| |
| |-
| |
| | 495.00 || 0.078245 || 0.679397 || 1.847599 || 2.252210 || 3.532038 || 4.920075
| |
| |-
| |
| | 495.50 || 0.076277 || 0.675597 || 1.826918 || 2.220414 || 3.514622 || 4.869704
| |
| |-
| |
| | 496.00 || 0.072367 || 0.670359 || 1.804214 || 2.194253 || 3.488290 || 4.824022
| |
| |-
| |
| | 496.50 || 0.067996 || 0.664834 || 1.783339 || 2.179730 || 3.457019 || 4.782542
| |
| |-
| |
| | 497.00 || 0.063936 || 0.661928 || 1.763531 || 2.175062 || 3.422583 || 4.748275
| |
| |-
| |
| | 497.50 || 0.061170 || 0.661056 || 1.745632 || 2.173006 || 3.382648 || 4.718746
| |
| |-
| |
| | 498.00 || 0.059845 || 0.661081 || 1.729174 || 2.172541 || 3.352517 || 4.685534
| |
| |-
| |
| | 498.50 || 0.058976 || 0.661538 || 1.712899 || 2.175151 || 3.330111 || 4.637928
| |
| |-
| |
| | 499.00 || 0.058475 || 0.661417 || 1.694747 || 2.180739 || 3.320443 || 4.576885
| |
| |-
| |
| | 499.50 || 0.059934 || 0.663243 || 1.673112 || 2.185242 || 3.325691 || 4.508523
| |
| |-
| |
| | 500.00 || 0.063000 || 0.668000 || 1.648000 || 2.185000 || 3.338000 || 4.437000
| |
| |}
| |
|
| |
| </center> | | </center> |
|
| |
|
Line 929: |
Line 110: |
|
| |
|
|
| |
|
| <center>[[Image:20150909 bonan uvvis abs corrected.png|thumb|center|800px]]</center> | | <center>[[Image:20151103 bonan corrected absorbance from 0909.png|thumb|center|800px]]</center> |
| | |
| The figure above shows the absorbance of each lysozyme standard as a function of the wavelength of incident light. The absorbance values were corrected for the water blank and dark noise.
| |
| | |
| | |
| <center>Table 4: Absorbance of Blank (HPLC Water) and Each Lysozyme Standard at a Wavelength of 280nm</center>
| |
| | |
| | |
| <center>[[Image:20150909 bonan uvvis conc vs abs.png|thumb|center|400px]]</center>
| |
|
| |
|
| The table above shows the absorbance of each lysozyme standard at a wavelength of 280nm. This data is shown graphically in the figure below. | | The figure above shows the absorbance of each lysozyme standard as a function of the wavelength of incident light. The absorbance values were first corrected for the water blank by subtracting the absorbance of the blank at each wavelength from that of the samples. The absorbance was then corrected for instrumental noise by subtracting the average of the last 100nm from every data point. |
|
| |
|
|
| |
|
Line 946: |
Line 119: |
|
| |
|
| <center> | | <center> |
| [[Image:20150923 bonan uvvis abs corrected.png|thumb|center|700px]] | | [[Image:20151103 bonan corrected calib curve from 0909.png|thumb|center|700px]] |
| </center> | | </center> |
|
| |
|
| The figure above shows the absorbance of lysozyme as a function of its concentration at a wavelength of incident light of 280nm. A trend line was added and set to a y-intercept of 0. The equation for this trend line, along with its r squared value, is shown on the graph. | | The figure above shows the absorbance of lysozyme as a function of its concentration at a wavelength of incident light of 280nm. A linear trend line was added with the y-intercept set to 0. The equation for this trend line, along with its r squared value, is shown on the graph. |
|
| |
|
| The slope of the line of best fit is 0.0337. This represents the molar absorptivity. Thus, the molar absorptivity was 0.0337µM<sup>-1</sup>cm<sup>-1</sup>, or 33.7mol<sup>-1</sup>cm<sup>-1</sup>. | | The slope of the line of best fit is 2364.1. This represents the molar absorptivity. Thus, the molar absorptivity was 2364.1µM<sup>-1</sup>cm<sup>-1</sup>, or 23.6mol<sup>-1</sup>cm<sup>-1</sup>. |
|
| |
|
| ===Fluorescence=== | | ===Fluorescence=== |
Line 968: |
Line 141: |
|
| |
|
|
| |
|
| <center>Table 5: Intensity of Re-emitted Light from Each Lysozyme Sample as a Function of Concentration (M)</center> | | <center>Table 3: Intensity of Re-emitted Light from Each Lysozyme Sample as a Function of Concentration (M)</center> |
|
| |
|
|
| |
|