User:Nicole Bonan/Notebook/Chem 571 Lab Notebook/2015/09/30: Difference between revisions
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# Finish taking fluorescence data from yesterday's lab | # Finish taking fluorescence data from yesterday's lab | ||
# Use a Bradford Assay in order to measure alpha-chymotrypsin degradation as a function of incubation time | # Use a Bradford Assay in order to measure alpha-chymotrypsin degradation as a function of incubation time | ||
==Protocol== | |||
Dr. Hartings's protocol for today is [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2015/09/29|here]]. We are following his protocols for fluorescence and for the Bradford Assay. | |||
===Fluorescence Analysis of Protease Degradation=== | |||
Yesterday, we measured the fluorescence of all of our samples of alpha-chymotrypsin + lysozyme. Today, we finished the fluorescence measurements for the alpha-chymotrypsin blanks as follows: | |||
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Revision as of 21:15, 3 October 2015
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ObjectiveThe purpose of today's lab work is to:
ProtocolDr. Hartings's protocol for today is here. We are following his protocols for fluorescence and for the Bradford Assay. Fluorescence Analysis of Protease DegradationYesterday, we measured the fluorescence of all of our samples of alpha-chymotrypsin + lysozyme. Today, we finished the fluorescence measurements for the alpha-chymotrypsin blanks as follows:
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