User:Nicole Bonan/Notebook/Chem 571 Lab Notebook/2015/10/07: Difference between revisions
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==Protocol== | ==Protocol== | ||
# First, we spun 4 samples of the AuNP fibers that Dr. Hartings synthesized on [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2015/10/02|September 2]] | # First, we spun down 4 samples of the AuNP fibers that Dr. Hartings synthesized on [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2015/10/02|September 2]] and pipetted off the supernatant | ||
# We then suspended the samples in 1 mL of Tris Buffer | # We then suspended the samples in 1 mL of Tris Buffer | ||
## The buffer was 50mM Tris and 20mM CaCl2 with pH=8 | ## The buffer was 50mM Tris and 20mM CaCl2 with pH=8 |
Revision as of 15:31, 11 October 2015
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ObjectiveThe purpose of today's lab work was to use the Ocean Optics spectrophotometer in order to measure how the absorbance of a sample of AuNP fiber sample changed over time as it was digested by alpha-chymotrypsin. Protocol
Data |