User:Nicole Bonan/Notebook/Chem 571 Lab Notebook/2015/10/07: Difference between revisions
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# After the second file was created, we added the alpha-chymotrypsin to the sample. | # After the second file was created, we added the alpha-chymotrypsin to the sample. | ||
==Data== | ==Data and Analysis== | ||
The Ocean Optics software measured the absorbance of our sample every two minutes using wavelengths of incident light from 190.96 to 891.81nm. We added the alpha-chymotrypsin 2 minutes into the experiment. | |||
In order to correct the data for the "blank", which was at 2 minutes (just before we added the alpha-chymotrypsin), I subtracted the absorbance value of the blank at each wavelength from the corresponding absorbance value of each "sample" (i.e. each 2 minute measurement). I then corrected for noise by subtracting the average absorbance values for the last 60nm of light from each "sample". | |||
Because the AuNP fibers absorb most strongly a wavelength of light of 530nm, I then plotted the absorbance value at an incident wavelength of 530nm as a function of time. The graph is shown below. | |||
<b><center>Figure 1: Absorbance of AuNP Fibers Degraded with alpha-Chymotrypsin as a Function of Time (min) at a Wavelength of 530nm</center></b> | |||
[[Image:20151008 bonan ocean optics final curve.png|thumb|center|700px]] | |||
From the figure above, it's clear that the AuNP fibers absorb much more greatly from about 0-5min, which was just before and after the alpha-chymotrypsin was added. After that, the absorbance decreases sharply and levels off at a low absorbance value of about 0.8. Because the absorbance decreases so sharply, and because the AuNP fibers are really the only substance that is absorbing light at this wavelength, the data indicates that alpha-chymotrypsin degrades AuNP fibers very rapidly and thoroughly. | |||
Revision as of 18:20, 11 October 2015
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ObjectiveThe purpose of today's lab work was to use the Ocean Optics spectrophotometer in order to measure how the absorbance of a sample of AuNP fiber sample changed over time as it was digested by alpha-chymotrypsin. Protocol
Data and AnalysisThe Ocean Optics software measured the absorbance of our sample every two minutes using wavelengths of incident light from 190.96 to 891.81nm. We added the alpha-chymotrypsin 2 minutes into the experiment.
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