User:Nicole Bonan/Notebook/Quantum Dots/2014/04/11: Difference between revisions
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==Objective== | ==Objective== | ||
Synthesize Mn-doped ZnS quantum dots using the synthesis described [http://pubs.acs.org/doi/full/10.1021/nn2024896 here]. None of the quantum dots | Synthesize Mn-doped ZnS quantum dots using the synthesis described [http://pubs.acs.org/doi/full/10.1021/nn2024896 here]. None of the quantum dots synthesized on 3/28/2014 fluoresced, so the dots were being re-synthesized this week using fresh 5mM Zn(CH<sub>3</sub>COO)<sub>2</sub>, 5mM Mn(CH<sub>3</sub>COO)<sub>2</sub>, and 5mM Na<sub>2</sub>S and using different mixing techniques (vortexing, hand-shaking, and mixing with a stir bar). | ||
==Description== | ==Description== | ||
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# Add 24μL of 5mM Mn((CH<sub>3</sub>COO)<sub>2</sub>) to the test tube | # Add 24μL of 5mM Mn((CH<sub>3</sub>COO)<sub>2</sub>) to the test tube | ||
# Add 200μL of 40mM NH4CH<sub>3</sub>COO to the test tube | # Add 200μL of 40mM NH4CH<sub>3</sub>COO to the test tube | ||
# Add 244μL of | # Add 244μL of 1.0mg/mL protein to the test tube | ||
# Add 250μL of 10mM NH<sub>4</sub>OH | # Add 250μL of 10mM NH<sub>4</sub>OH | ||
## This should bring the pH up to 8.2. Check on this with a pH strip | ## This should bring the pH up to 8.2. Check on this with a pH strip | ||
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==Data== | ==Data== | ||
< | |||
Test tubes | |||
*"NB1" was synthesized using the procedure above, except that 510µL NH<sub>4</sub>OH and 1346µL H<sub>2</sub>O were used. NB1 was not shaken while the reagents were added, except for when the Na<sub>2</sub>S was added. | |||
*"NB2" was synthesized using the procedure above, except that 550µL NH<sub>4</sub>OH and 1306µL H<sub>2</sub>O were used. NB2 was hand-shaken after each reagent was added. |
Revision as of 18:57, 17 April 2014
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ObjectiveSynthesize Mn-doped ZnS quantum dots using the synthesis described here. None of the quantum dots synthesized on 3/28/2014 fluoresced, so the dots were being re-synthesized this week using fresh 5mM Zn(CH3COO)2, 5mM Mn(CH3COO)2, and 5mM Na2S and using different mixing techniques (vortexing, hand-shaking, and mixing with a stir bar). DescriptionSynthesis Protocol for Mn-doped ZnS nanocrystals with an Mn:Zn ratio of 1:7.5 and a final (Zn+Mn) concentration of 0.4mM
DataTest tubes
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