User:Nidhi N. Rao/Notebook/Robocast biodegradable 3D scaffolds/2013/05/02: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Robocast biodegradable 3D scaffolds</span> | ||
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== | ==Experiment 1== | ||
Aim – To dissolve PCL in chloroform and check for dissolving time, temperature | |||
Materials – | |||
0.235 g and 0.479g of PCL for 10 and 20% mixture respectively (Sigma Aldrich MW -70,000 to 90,000; 5g bottle) | |||
0.235 ml of Chloroform HPLC grade | |||
Method- | |||
The method was adopted from http://www4.ncsu.edu/~fjliving/docs/Bone%20Tissue%20Scaffold%20in%20PCL.pdf | |||
Mixtures of 10% PCL in chloroform were made by combining 0.235g of PCL with 0.235 ml of chloroform. Mixtures of 20% PCL in chloroform were made by combining 0.479 g of PCL with 0.235 ml of chloroform. The beakers were sealed with parafilm and the mixture was stirred for 25 minutes for complete dissolution of pellets using a magnetic stirrer at RT. | |||
Results: | |||
The 20% mixture turned too viscous and stretchy for use in robocaster. Although the 10% mixture was a bit runny, it turned slightly more viscous when exposed to air, which might be a good consistency for robocasting. | |||
Revision as of 08:25, 21 June 2013
 Robocast biodegradable 3D scaffolds
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page |
Experiment 1Aim – To dissolve PCL in chloroform and check for dissolving time, temperature Materials – 0.235 g and 0.479g of PCL for 10 and 20% mixture respectively (Sigma Aldrich MW -70,000 to 90,000; 5g bottle) 0.235 ml of Chloroform HPLC grade Method- The method was adopted from http://www4.ncsu.edu/~fjliving/docs/Bone%20Tissue%20Scaffold%20in%20PCL.pdf Mixtures of 10% PCL in chloroform were made by combining 0.235g of PCL with 0.235 ml of chloroform. Mixtures of 20% PCL in chloroform were made by combining 0.479 g of PCL with 0.235 ml of chloroform. The beakers were sealed with parafilm and the mixture was stirred for 25 minutes for complete dissolution of pellets using a magnetic stirrer at RT. Results: The 20% mixture turned too viscous and stretchy for use in robocaster. Although the 10% mixture was a bit runny, it turned slightly more viscous when exposed to air, which might be a good consistency for robocasting.
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