User:Nikolai Eroshenko

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Publications)
Line 8: Line 8:
== Publications ==
== Publications ==
-
<span style="font-size:115%">'''Gene assembly from chip-synthesized oligonucleotides''' ([http://dl.dropbox.com/u/5389110/GeneAssemblyFromChipSynthesizedOligonucleotides%20(1).pdf PDF])
+
<span style="font-size:115%">'''Gene assembly from chip-synthesized oligonucleotides''' ([http://dl.dropbox.com/u/5389110/GeneAssemblyFromChipSynthesizedOligonucleotides%20(1).pdf PDF]) </span>
Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church, ''Current Protocols in Chemical Biology'' 4 (2012)
Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church, ''Current Protocols in Chemical Biology'' 4 (2012)
 +
* <span style="font-size:85%"> Gene Assembly by Subpool PCR (GASP) oligo design [https://bitbucket.org/skosuri/gasp/src/364d21def3ec/cleaned-up-scripts/gaspserver/ scripts] and [http://synbiosis.med.harvard.edu:8080/gaspserver.rpy server] </span>
* <span style="font-size:85%"> Gene Assembly by Subpool PCR (GASP) oligo design [https://bitbucket.org/skosuri/gasp/src/364d21def3ec/cleaned-up-scripts/gaspserver/ scripts] and [http://synbiosis.med.harvard.edu:8080/gaspserver.rpy server] </span>
-
<span style="font-size:115%"> Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors ([http://dl.dropbox.com/u/5389110/gt2011134a.pdf PDF])
+
<span style="font-size:115%"> '''Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors''' ([http://dl.dropbox.com/u/5389110/gt2011134a.pdf PDF])</span>
Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao, ''Gene Therapy'' AOP (2011)  
Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao, ''Gene Therapy'' AOP (2011)  
-
<span style="font-size:115%">Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips ([http://dl.dropbox.com/u/5389110/nbt.1716.pdf PDF])
+
<span style="font-size:115%">'''Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips''' ([http://dl.dropbox.com/u/5389110/nbt.1716.pdf PDF])</span>
 +
 
Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church, ''Nature Biotechnology'' 28:1295 (2010)  
Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church, ''Nature Biotechnology'' 28:1295 (2010)  
 +
* <span style="font-size:85%">[http://www.nature.com/nbt/journal/v28/n12/extref/nbt.1716-S1.pdf Supplementary Text and Figures] (.pdf)
* <span style="font-size:85%">[http://www.nature.com/nbt/journal/v28/n12/extref/nbt.1716-S1.pdf Supplementary Text and Figures] (.pdf)
* <span style="font-size:85%">[http://www.nature.com/nbt/journal/v28/n12/extref/nbt.1716-S2.xls Supplementary Sequences] (.xls)
* <span style="font-size:85%">[http://www.nature.com/nbt/journal/v28/n12/extref/nbt.1716-S2.xls Supplementary Sequences] (.xls)
Line 24: Line 27:
* <span style="font-size:85%">Commentary: [http://dl.dropbox.com/u/5389110/nbt.1723.pdf Nature Biotechnology]; [http://dl.dropbox.com/u/5389110/nrg2924.pdf Nature Reviews Genetics]
* <span style="font-size:85%">Commentary: [http://dl.dropbox.com/u/5389110/nbt.1723.pdf Nature Biotechnology]; [http://dl.dropbox.com/u/5389110/nrg2924.pdf Nature Reviews Genetics]
-
<span style="font-size:115%">Role of bioinspired polymers in determination of pluripotent stem cell fate ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756563/pdf/nihms-132034.pdf PDF])
+
<span style="font-size:115%">'''Role of bioinspired polymers in determination of pluripotent stem cell fate''' ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756563/pdf/nihms-132034.pdf PDF]) </span>
 +
 
Sheena Abraham, Nikolai Eroshenko & Raj R. Rao, ''Regenerative Medicine'' 4:561 (2009)
Sheena Abraham, Nikolai Eroshenko & Raj R. Rao, ''Regenerative Medicine'' 4:561 (2009)
 +
 +
==Labs==
 +
[http://arep.med.harvard.edu/ George Church] (2009-now)
 +
 +
[http://wyss.harvard.edu/ Wyss Institute] (2009-now)
 +
 +
[http://www.engineering.vcu.edu/rao-lab/New_Rao_Site/Raohome.php Raj Rao] (2006-2008)
 +
 +
[http://plaza.ufl.edu/curtis.taylor/Florida_Nanoscale_Manufacturing/Home.html Curtis Taylor] (2006-2007)
 +
 +
==Education==
 +
[http://seas.harvard.edu/ Harvard University] (2008-now) PhD candidate, Bioengineering
 +
 +
[http://www.egr.vcu.edu/Page.aspx?id=93 Virginia Commonwealth University] (2005-2008) BS, Biomedical Engineering
 +
 +
==Contact==
 +
eroshenkona AT gmail
 +
 +
Nikolai Eroshenko
 +
77 Ave. Louis Pasteur
 +
NRB 238
 +
Boston, MA 02115

Revision as of 08:49, 3 June 2012

Contents

Research

I am building new technologies for basic and applied biology. Long-term research interests include:

  • fully synthetic (i.e. non-immunogenic) gene therapy vectors that integrate genes into a precise location on the genome
  • cheaper, faster, and more accurate gene synthesis
  • library vs. library screens which quantify the strength of protein-protein and protein-DNA interactions

Publications

Gene assembly from chip-synthesized oligonucleotides (PDF)

Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church, Current Protocols in Chemical Biology 4 (2012)

  • Gene Assembly by Subpool PCR (GASP) oligo design scripts and server

Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors (PDF)

Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao, Gene Therapy AOP (2011)

Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips (PDF)

Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church, Nature Biotechnology 28:1295 (2010)

Role of bioinspired polymers in determination of pluripotent stem cell fate (PDF)

Sheena Abraham, Nikolai Eroshenko & Raj R. Rao, Regenerative Medicine 4:561 (2009)

Labs

George Church (2009-now)

Wyss Institute (2009-now)

Raj Rao (2006-2008)

Curtis Taylor (2006-2007)

Education

Harvard University (2008-now) PhD candidate, Bioengineering

Virginia Commonwealth University (2005-2008) BS, Biomedical Engineering

Contact

eroshenkona AT gmail

Nikolai Eroshenko 77 Ave. Louis Pasteur NRB 238 Boston, MA 02115

Personal tools