User:Nikolai Eroshenko: Difference between revisions
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== Publications == | == Publications == | ||
<span style="font-size:115%">'''Gene assembly from chip-synthesized oligonucleotides''' ([http://dl.dropbox.com/u/5389110/GeneAssemblyFromChipSynthesizedOligonucleotides%20(1).pdf PDF]) | <span style="font-size:115%">'''Gene assembly from chip-synthesized oligonucleotides''' ([http://dl.dropbox.com/u/5389110/GeneAssemblyFromChipSynthesizedOligonucleotides%20(1).pdf PDF]) </span> | ||
Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church, ''Current Protocols in Chemical Biology'' 4 (2012) | Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church, ''Current Protocols in Chemical Biology'' 4 (2012) | ||
* <span style="font-size:85%"> Gene Assembly by Subpool PCR (GASP) oligo design [https://bitbucket.org/skosuri/gasp/src/364d21def3ec/cleaned-up-scripts/gaspserver/ scripts] and [http://synbiosis.med.harvard.edu:8080/gaspserver.rpy server] </span> | * <span style="font-size:85%"> Gene Assembly by Subpool PCR (GASP) oligo design [https://bitbucket.org/skosuri/gasp/src/364d21def3ec/cleaned-up-scripts/gaspserver/ scripts] and [http://synbiosis.med.harvard.edu:8080/gaspserver.rpy server] </span> | ||
<span style="font-size:115%"> Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors ([http://dl.dropbox.com/u/5389110/gt2011134a.pdf PDF]) | <span style="font-size:115%"> '''Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors''' ([http://dl.dropbox.com/u/5389110/gt2011134a.pdf PDF])</span> | ||
Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao, ''Gene Therapy'' AOP (2011) | Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao, ''Gene Therapy'' AOP (2011) | ||
<span style="font-size:115%">Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips ([http://dl.dropbox.com/u/5389110/nbt.1716.pdf PDF]) | <span style="font-size:115%">'''Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips''' ([http://dl.dropbox.com/u/5389110/nbt.1716.pdf PDF])</span> | ||
Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church, ''Nature Biotechnology'' 28:1295 (2010) | Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church, ''Nature Biotechnology'' 28:1295 (2010) | ||
* <span style="font-size:85%">[http://www.nature.com/nbt/journal/v28/n12/extref/nbt.1716-S1.pdf Supplementary Text and Figures] (.pdf) | * <span style="font-size:85%">[http://www.nature.com/nbt/journal/v28/n12/extref/nbt.1716-S1.pdf Supplementary Text and Figures] (.pdf) | ||
* <span style="font-size:85%">[http://www.nature.com/nbt/journal/v28/n12/extref/nbt.1716-S2.xls Supplementary Sequences] (.xls) | * <span style="font-size:85%">[http://www.nature.com/nbt/journal/v28/n12/extref/nbt.1716-S2.xls Supplementary Sequences] (.xls) | ||
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* <span style="font-size:85%">Commentary: [http://dl.dropbox.com/u/5389110/nbt.1723.pdf Nature Biotechnology]; [http://dl.dropbox.com/u/5389110/nrg2924.pdf Nature Reviews Genetics] | * <span style="font-size:85%">Commentary: [http://dl.dropbox.com/u/5389110/nbt.1723.pdf Nature Biotechnology]; [http://dl.dropbox.com/u/5389110/nrg2924.pdf Nature Reviews Genetics] | ||
<span style="font-size:115%">Role of bioinspired polymers in determination of pluripotent stem cell fate ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756563/pdf/nihms-132034.pdf PDF]) | <span style="font-size:115%">'''Role of bioinspired polymers in determination of pluripotent stem cell fate''' ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756563/pdf/nihms-132034.pdf PDF]) </span> | ||
Sheena Abraham, Nikolai Eroshenko & Raj R. Rao, ''Regenerative Medicine'' 4:561 (2009) | Sheena Abraham, Nikolai Eroshenko & Raj R. Rao, ''Regenerative Medicine'' 4:561 (2009) | ||
==Labs== | |||
[http://arep.med.harvard.edu/ George Church] (2009-now) | |||
[http://wyss.harvard.edu/ Wyss Institute] (2009-now) | |||
[http://www.engineering.vcu.edu/rao-lab/New_Rao_Site/Raohome.php Raj Rao] (2006-2008) | |||
[http://plaza.ufl.edu/curtis.taylor/Florida_Nanoscale_Manufacturing/Home.html Curtis Taylor] (2006-2007) | |||
==Education== | |||
[http://seas.harvard.edu/ Harvard University] (2008-now) PhD candidate, Bioengineering | |||
[http://www.egr.vcu.edu/Page.aspx?id=93 Virginia Commonwealth University] (2005-2008) BS, Biomedical Engineering | |||
==Contact== | |||
eroshenkona AT gmail | |||
Nikolai Eroshenko | |||
77 Ave. Louis Pasteur | |||
NRB 238 | |||
Boston, MA 02115 |
Revision as of 06:49, 3 June 2012
Research
I am building new technologies for basic and applied biology. Long-term research interests include:
- fully synthetic (i.e. non-immunogenic) gene therapy vectors that integrate genes into a precise location on the genome
- cheaper, faster, and more accurate gene synthesis
- library vs. library screens which quantify the strength of protein-protein and protein-DNA interactions
Publications
Gene assembly from chip-synthesized oligonucleotides (PDF)
Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church, Current Protocols in Chemical Biology 4 (2012)
Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors (PDF)
Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao, Gene Therapy AOP (2011)
Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips (PDF)
Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church, Nature Biotechnology 28:1295 (2010)
- Supplementary Text and Figures (.pdf)
- Supplementary Sequences (.xls)
- Runner-up for Science 2011 Breakthrough of the Year
- Commentary: Nature Biotechnology; Nature Reviews Genetics
Role of bioinspired polymers in determination of pluripotent stem cell fate (PDF)
Sheena Abraham, Nikolai Eroshenko & Raj R. Rao, Regenerative Medicine 4:561 (2009)
Labs
George Church (2009-now)
Wyss Institute (2009-now)
Raj Rao (2006-2008)
Curtis Taylor (2006-2007)
Education
Harvard University (2008-now) PhD candidate, Bioengineering
Virginia Commonwealth University (2005-2008) BS, Biomedical Engineering
Contact
eroshenkona AT gmail
Nikolai Eroshenko 77 Ave. Louis Pasteur NRB 238 Boston, MA 02115