User:Nikolai Eroshenko: Difference between revisions
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* <span style="font-size:85%"> Gene Assembly by Subpool PCR (GASP) oligo design [https://bitbucket.org/skosuri/gasp/src/364d21def3ec/cleaned-up-scripts/gaspserver/ scripts] and [http://synbiosis.med.harvard.edu:8080/gaspserver.rpy server] </span> | * <span style="font-size:85%"> Gene Assembly by Subpool PCR (GASP) oligo design [https://bitbucket.org/skosuri/gasp/src/364d21def3ec/cleaned-up-scripts/gaspserver/ scripts] and [http://synbiosis.med.harvard.edu:8080/gaspserver.rpy server] </span> | ||
<span style="font-size:115%">Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips | <span style="font-size:115%">Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips ([http://dl.dropbox.com/u/5389110/nbt.1716.pdf PDF])</span> | ||
<span style="font-size:85%">Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church, ''Nature Biotechnology'' 28:1295 (2010)</span> | <span style="font-size:85%">Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church, ''Nature Biotechnology'' 28:1295 (2010)</span> | ||
Revision as of 09:13, 20 February 2013
Research
I am building new technologies for basic and applied biology. Long-term research interests include:
- human transgene vectors that integrate genes into precise locations on the genome
- cheaper, faster, and more accurate gene synthesis
- library vs. library screens which quantify the strength of protein-protein and protein-DNA interactions
Publications
Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors (abstract)
Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao, Gene Therapy 19:469 (2012)
Gene assembly from chip-synthesized oligonucleotides (PDF)
Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church, Current Protocols in Chemical Biology 4:1 (2012)
Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips (PDF)
Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church, Nature Biotechnology 28:1295 (2010)
- Supplementary Text and Figures (.pdf)
- Supplementary Sequences (.xls)
- Commentary: Nature Biotechnology; Nature Reviews Genetics
Role of bioinspired polymers in determination of pluripotent stem cell fate (PDF)
Sheena Abraham, Nikolai Eroshenko & Raj R. Rao, Regenerative Medicine 4:561 (2009)
Patents
Orthogonal amplification and assembly of nucleic acid sequences, PCT/US2011/057075, pending
Labs
George Church (2009-now)
Wyss Institute (2009-2011)
Raj Rao (2006-2008)
Curtis Taylor (2006-2007)
Education
Harvard University (2008-now) PhD candidate, Bioengineering
Virginia Commonwealth University (2005-2008) BS, Biomedical Engineering
Contact
eroshenkona AT gmail
Nikolai Eroshenko
77 Ave. Louis Pasteur
NRB 238
Boston, MA 02115
