User:Nikolai Eroshenko: Difference between revisions

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"All there is to thinking, is seeing something noticeable which makes you see something you weren’t noticing, which makes you see something that isn’t even visible." -Norman Maclean
==Education==
[http://www.seas.harvard.edu/academics/graduate Harvard University] (2008-now) PhD candidate, Engineering Sciences: Bioengineering


==Interests==
[http://www.egr.vcu.edu/departments-centers/biomedical-engineering/ Virginia Commonwealth University] (2005-2008) BS, Biomedical Engineering
*gene synthesis
*genome engineering
*protein design
*molecular biology methods development
*plant-based protein production


==Publications==
==Contact==
[http://www.people.fas.harvard.edu/~amarbles//docs/GeneAssemblyFromChipSynthesizedOligonucleotides.pdf '''Gene assembly from chip-synthesized oligonucleotides''']<br>
eroshenkona AT gmail
'''Nikolai Eroshenko'''*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George_Church, ''Current Protocols in Chemical Biology'' '''4''' (2012)


[http://dl.dropbox.com/u/5389110/gt2011134a.pdf '''Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors''']<br>
77 Ave. Louis Pasteur<br>  
Shilpa Iyer, Ena Xiao, Khaled Alsayegh, '''Nikolai Eroshenko''', Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao, ''Gene Therapy'' AOP (2011)
NRB 238 <br>
<br>
Boston, MA 02115


[http://dl.dropbox.com/u/5389110/nbt.1716.pdf '''Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips''']<br>
[http://scholar.google.com/citations?user=FxBA_wQAAAAJ&hl=en Google Scholar page]
Sriram Kosuri*, '''Nikolai Eroshenko'''*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George_Church, ''Nature Biotechnology'' '''28''':1295  (2010) [http://www.nature.com/nbt/journal/v28/n12/covers/index.html cover]
<br>


[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756563/pdf/nihms-132034.pdf '''Role of bioinspired polymers in determination of pluripotent stem cell fate''']<br>
== Research Interests ==
Sheena Abraham, '''Nikolai Eroshenko''' & Raj R. Rao, ''Regenerative Medicine'' '''4''':561 (2009)
synthetic evolution, gene therapy, gene synthesis, origins of life, protein design


==Affiliations==
== Publications ==
G4 Harvard [http://www.seas.harvard.edu/ SEAS] Bioengineering
6. '''Nikolai Eroshenko''', George Church (2013) [http://arep.med.harvard.edu/pdf/Eroshenko_rec_13.pdf Mutants of Cre recombinase with improved accuracy.] Nat Commun 4:2509
[http://arep.med.harvard.edu/ Church Lab] ([http://genetics.med.harvard.edu/ HMS Genetics], NRB 238/[http://wyss.harvard.edu/ Wyss Institute])


===Previous===
5. '''Nikolai Eroshenko'''*, Rukmani Ramachandran*, Vamsi Yadavalli, Raj R. Rao (2013) [http://www.jbioleng.org/content/pdf/1754-1611-7-7.pdf Effect of substrate stiffness on early human embryonic stem cell differentiation.] J Biol Eng 7:7
[http://zlab.mit.edu/ Feng Zhang's lab] - McGovern Institute/Broad Institute, MIT <br>
 
[http://diseasebiophysics.seas.harvard.edu/ Kit Parker's lab] - Harvard Bioengineering <br>
4. Shilpa Iyer, Ena Xiao, Khaled Alsayegh, '''Nikolai Eroshenko''', Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao (2012) [https://dl.dropboxusercontent.com/u/5389110/gt2011134a.pdf Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors.] Gene Ther 19:469
[http://www.engineering.vcu.edu/rao-lab/New_Rao_Site/Raohome.php Raj Rao's lab] - VCU Chemical Engineering <br>
 
[http://plaza.ufl.edu/curtis.taylor/Florida_Nanoscale_Manufacturing/Home.html Curtis Taylor's lab] - VCU Mechanical Engineering (now at UF)
3. '''Nikolai Eroshenko'''*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church (2012) [http://dl.dropbox.com/u/5389110/GeneAssemblyFromChipSynthesizedOligonucleotides%20(1).pdf Gene assembly from chip-synthesized oligonucleotides.] Curr Prot Chem Biol 4:1
 
2. Sriram Kosuri*, '''Nikolai Eroshenko'''*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church (2010) [http://dl.dropbox.com/u/5389110/nbt.1716.pdf Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips.] Nat Biotechnol 28:1295
 
1. Sheena Abraham, '''Nikolai Eroshenko''' & Raj R. Rao (2009) [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756563/pdf/nihms-132034.pdf Role of bioinspired polymers in determination of pluripotent stem cell fate.] Regen Med 4:561
 
==Patents==
1. George Church, Sriram Kosuri, '''Nikolai Eroshenko''', [http://patentscope.wipo.int/search/docservicepdf_pct/id00000019172253.pdf Orthogonal amplification and assembly of nucleic acid sequences] International Patent Application No. PCT/US2011/05707 (Harvard)
 
== News ==
[http://www.nature.com/nmeth/journal/v10/n11/full/nmeth.2708.html Highly accurate Cre variants], Nature Methods, Oct. 30 2013
 
[http://www.biotechniques.com/news/Refining-a-Recombinase/biotechniques-347492.html#.UmV-fZSifTw Refining a Recombinase], BioTechniques, Oct. 20 2013, Jeffrey M. Perkel
 
[http://www.nature.com/nrg/journal/v12/n1/full/nrg2924.html Technology: Making more genes, better and for less], Nature Reviews Genetics, Jan. 2011, Louisa Flintoft
 
[http://www.nature.com/scibx/journal/v4/n1/full/scibx.2011.28.html Gene construction using microchip-based DNA synthesis and PCR amplification], SciBX, Jan. 6 2011
 
[http://www.biotechniques.com/news/Technology-for-the-everyman/biotechniques-307796.html Technology for the everyman], Biotechniques, Dec. 23 2010, Suzanne E. Winter
 
[http://www.nature.com/nbt/journal/v28/n12/full/nbt.1723.html Megabases for kilodollars], Nature Biotechnology, Dec. 07 2010, Mikkel Algire, Radha Krishnakumar & Chuck Merryman

Revision as of 08:23, 26 August 2014

Education

Harvard University (2008-now) PhD candidate, Engineering Sciences: Bioengineering

Virginia Commonwealth University (2005-2008) BS, Biomedical Engineering

Contact

eroshenkona AT gmail

77 Ave. Louis Pasteur
NRB 238
Boston, MA 02115

Google Scholar page

Research Interests

synthetic evolution, gene therapy, gene synthesis, origins of life, protein design

Publications

6. Nikolai Eroshenko, George Church (2013) Mutants of Cre recombinase with improved accuracy. Nat Commun 4:2509

5. Nikolai Eroshenko*, Rukmani Ramachandran*, Vamsi Yadavalli, Raj R. Rao (2013) Effect of substrate stiffness on early human embryonic stem cell differentiation. J Biol Eng 7:7

4. Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao (2012) Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors. Gene Ther 19:469

3. Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church (2012) Gene assembly from chip-synthesized oligonucleotides. Curr Prot Chem Biol 4:1

2. Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church (2010) Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips. Nat Biotechnol 28:1295

1. Sheena Abraham, Nikolai Eroshenko & Raj R. Rao (2009) Role of bioinspired polymers in determination of pluripotent stem cell fate. Regen Med 4:561

Patents

1. George Church, Sriram Kosuri, Nikolai Eroshenko, Orthogonal amplification and assembly of nucleic acid sequences International Patent Application No. PCT/US2011/05707 (Harvard)

News

Highly accurate Cre variants, Nature Methods, Oct. 30 2013

Refining a Recombinase, BioTechniques, Oct. 20 2013, Jeffrey M. Perkel

Technology: Making more genes, better and for less, Nature Reviews Genetics, Jan. 2011, Louisa Flintoft

Gene construction using microchip-based DNA synthesis and PCR amplification, SciBX, Jan. 6 2011

Technology for the everyman, Biotechniques, Dec. 23 2010, Suzanne E. Winter

Megabases for kilodollars, Nature Biotechnology, Dec. 07 2010, Mikkel Algire, Radha Krishnakumar & Chuck Merryman

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