User:Nikolai Eroshenko: Difference between revisions

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"All there is to thinking, is seeing something noticeable which makes you see something you weren’t noticing, which makes you see something that isn’t even visible." -Norman Maclean
==Education==
[http://arep.med.harvard.edu/ Church lab, Harvard Medical School] (2014-currently) postdoc


==Interests==
[http://www.seas.harvard.edu/academics/graduate Harvard University] (2008-2014) PhD candidate, Engineering Sciences: Bioengineering
*gene synthesis
*genome engineering
*protein design
*molecular biology methods development
*plant-based protein production


==Publications==
[http://www.egr.vcu.edu/departments-centers/biomedical-engineering/ Virginia Commonwealth University] (2005-2008) BS, Biomedical Engineering
[http://www.people.fas.harvard.edu/~amarbles//docs/GeneAssemblyFromChipSynthesizedOligonucleotides.pdf '''Gene assembly from chip-synthesized oligonucleotides''']<br>
'''Nikolai Eroshenko'''*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George_Church, ''Current Protocols in Chemical Biology'' '''4''' (2012)  


[http://dl.dropbox.com/u/5389110/gt2011134a.pdf '''Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors''']<br>
==Contact==
Shilpa Iyer, Ena Xiao, Khaled Alsayegh, '''Nikolai Eroshenko''', Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao, ''Gene Therapy'' AOP (2011)
eroshenkona AT gmail
<br>


[http://dl.dropbox.com/u/5389110/nbt.1716.pdf '''Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips''']<br>
77 Ave. Louis Pasteur<br>  
Sriram Kosuri*, '''Nikolai Eroshenko'''*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George_Church, ''Nature Biotechnology'' '''28''':1295  (2010) [http://www.nature.com/nbt/journal/v28/n12/covers/index.html cover]
NRB 238 <br>
<br>
Boston, MA 02115


[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756563/pdf/nihms-132034.pdf '''Role of bioinspired polymers in determination of pluripotent stem cell fate''']<br>
[http://scholar.google.com/citations?user=FxBA_wQAAAAJ&hl=en Google Scholar page]
Sheena Abraham, '''Nikolai Eroshenko''' & Raj R. Rao, ''Regenerative Medicine'' '''4''':561 (2009)


==Affiliations==
== Research Interests ==
G4 Harvard [http://www.seas.harvard.edu/ SEAS] Bioengineering
synthetic evolution, gene therapy, gene synthesis, origins of life, protein design
[http://arep.med.harvard.edu/ Church Lab] ([http://genetics.med.harvard.edu/ HMS Genetics], NRB 238/[http://wyss.harvard.edu/ Wyss Institute])


===Previous===
== Publications ==
[http://zlab.mit.edu/ Feng Zhang's lab] - McGovern Institute/Broad Institute, MIT <br>
6. '''Nikolai Eroshenko''', George Church (2013) [http://arep.med.harvard.edu/pdf/Eroshenko_rec_13.pdf Mutants of Cre recombinase with improved accuracy.] Nat Commun 4:2509
[http://diseasebiophysics.seas.harvard.edu/ Kit Parker's lab] - Harvard Bioengineering <br>
 
[http://www.engineering.vcu.edu/rao-lab/New_Rao_Site/Raohome.php Raj Rao's lab] - VCU Chemical Engineering <br>
5. '''Nikolai Eroshenko'''*, Rukmani Ramachandran*, Vamsi Yadavalli, Raj R. Rao (2013) [http://www.jbioleng.org/content/pdf/1754-1611-7-7.pdf Effect of substrate stiffness on early human embryonic stem cell differentiation.] J Biol Eng 7:7
[http://plaza.ufl.edu/curtis.taylor/Florida_Nanoscale_Manufacturing/Home.html Curtis Taylor's lab] - VCU Mechanical Engineering (now at UF)
 
4. Shilpa Iyer, Ena Xiao, Khaled Alsayegh, '''Nikolai Eroshenko''', Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao (2012) [https://dl.dropboxusercontent.com/u/5389110/gt2011134a.pdf Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors.] Gene Ther 19:469
 
3. '''Nikolai Eroshenko'''*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church (2012) [http://dl.dropbox.com/u/5389110/GeneAssemblyFromChipSynthesizedOligonucleotides%20(1).pdf Gene assembly from chip-synthesized oligonucleotides.] Curr Prot Chem Biol 4:1
 
2. Sriram Kosuri*, '''Nikolai Eroshenko'''*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church (2010) [http://dl.dropbox.com/u/5389110/nbt.1716.pdf Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips.] Nat Biotechnol 28:1295
 
1. Sheena Abraham, '''Nikolai Eroshenko''' & Raj R. Rao (2009) [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756563/pdf/nihms-132034.pdf Role of bioinspired polymers in determination of pluripotent stem cell fate.] Regen Med 4:561
 
==Patents==
2. '''Nikolai Eroshenko''', George Church (2014) [http://patentscope.wipo.int/search/en/WO2014158593 Mutants of cre recombinase] (Harvard)
 
1. George Church, Sriram Kosuri, '''Nikolai Eroshenko''' (2013) [http://patentscope.wipo.int/search/docservicepdf_pct/id00000019172253.pdf Orthogonal amplification and assembly of nucleic acid sequences] (Harvard)
 
== News ==
[http://www.nature.com/nmeth/journal/v10/n11/full/nmeth.2708.html Highly accurate Cre variants], Nature Methods, Oct. 30 2013
 
[http://www.biotechniques.com/news/Refining-a-Recombinase/biotechniques-347492.html#.UmV-fZSifTw Refining a Recombinase], BioTechniques, Oct. 20 2013, Jeffrey M. Perkel
 
[http://www.nature.com/nrg/journal/v12/n1/full/nrg2924.html Technology: Making more genes, better and for less], Nature Reviews Genetics, Jan. 2011, Louisa Flintoft
 
[http://www.nature.com/scibx/journal/v4/n1/full/scibx.2011.28.html Gene construction using microchip-based DNA synthesis and PCR amplification], SciBX, Jan. 6 2011
 
[http://www.biotechniques.com/news/Technology-for-the-everyman/biotechniques-307796.html Technology for the everyman], Biotechniques, Dec. 23 2010, Suzanne E. Winter
 
[http://www.nature.com/nbt/journal/v28/n12/full/nbt.1723.html Megabases for kilodollars], Nature Biotechnology, Dec. 07 2010, Mikkel Algire, Radha Krishnakumar & Chuck Merryman

Revision as of 17:10, 16 January 2015

Education

Church lab, Harvard Medical School (2014-currently) postdoc

Harvard University (2008-2014) PhD candidate, Engineering Sciences: Bioengineering

Virginia Commonwealth University (2005-2008) BS, Biomedical Engineering

Contact

eroshenkona AT gmail

77 Ave. Louis Pasteur
NRB 238
Boston, MA 02115

Google Scholar page

Research Interests

synthetic evolution, gene therapy, gene synthesis, origins of life, protein design

Publications

6. Nikolai Eroshenko, George Church (2013) Mutants of Cre recombinase with improved accuracy. Nat Commun 4:2509

5. Nikolai Eroshenko*, Rukmani Ramachandran*, Vamsi Yadavalli, Raj R. Rao (2013) Effect of substrate stiffness on early human embryonic stem cell differentiation. J Biol Eng 7:7

4. Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao (2012) Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors. Gene Ther 19:469

3. Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church (2012) Gene assembly from chip-synthesized oligonucleotides. Curr Prot Chem Biol 4:1

2. Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church (2010) Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips. Nat Biotechnol 28:1295

1. Sheena Abraham, Nikolai Eroshenko & Raj R. Rao (2009) Role of bioinspired polymers in determination of pluripotent stem cell fate. Regen Med 4:561

Patents

2. Nikolai Eroshenko, George Church (2014) Mutants of cre recombinase (Harvard)

1. George Church, Sriram Kosuri, Nikolai Eroshenko (2013) Orthogonal amplification and assembly of nucleic acid sequences (Harvard)

News

Highly accurate Cre variants, Nature Methods, Oct. 30 2013

Refining a Recombinase, BioTechniques, Oct. 20 2013, Jeffrey M. Perkel

Technology: Making more genes, better and for less, Nature Reviews Genetics, Jan. 2011, Louisa Flintoft

Gene construction using microchip-based DNA synthesis and PCR amplification, SciBX, Jan. 6 2011

Technology for the everyman, Biotechniques, Dec. 23 2010, Suzanne E. Winter

Megabases for kilodollars, Nature Biotechnology, Dec. 07 2010, Mikkel Algire, Radha Krishnakumar & Chuck Merryman

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