User:Nikolai Eroshenko: Difference between revisions

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== Research ==


I am building new technologies for basic and applied biology. Long-term research interests include:
* fully synthetic (i.e. non-immunogenic) gene therapy vectors that integrate genes into a precise location on the genome
* cheaper, faster, and more accurate gene synthesis
* library vs. library screens which quantify the strength of protein-protein and protein-DNA interactions
== Publications ==
<span style="font-size:115%">'''Gene assembly from chip-synthesized oligonucleotides''' ([http://dl.dropbox.com/u/5389110/GeneAssemblyFromChipSynthesizedOligonucleotides%20(1).pdf PDF])
Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church, ''Current Protocols in Chemical Biology'' 4 (2012)
* <span style="font-size:85%"> Gene Assembly by Subpool PCR (GASP) oligo design [https://bitbucket.org/skosuri/gasp/src/364d21def3ec/cleaned-up-scripts/gaspserver/ scripts] and [http://synbiosis.med.harvard.edu:8080/gaspserver.rpy server] </span>
<span style="font-size:115%"> Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors ([http://dl.dropbox.com/u/5389110/gt2011134a.pdf PDF])
Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao, ''Gene Therapy'' AOP (2011)
<span style="font-size:115%">Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips ([http://dl.dropbox.com/u/5389110/nbt.1716.pdf PDF])
Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church, ''Nature Biotechnology'' 28:1295 (2010)
* <span style="font-size:85%">[http://www.nature.com/nbt/journal/v28/n12/extref/nbt.1716-S1.pdf Supplementary Text and Figures] (.pdf)
* <span style="font-size:85%">[http://www.nature.com/nbt/journal/v28/n12/extref/nbt.1716-S2.xls Supplementary Sequences] (.xls)
* <span style="font-size:85%">[http://dl.dropbox.com/u/5389110/Science-2010-News%20Focus-1605-7.pdf Runner-up for Science 2011 Breakthrough of the Year]
* <span style="font-size:85%">Commentary: [http://dl.dropbox.com/u/5389110/nbt.1723.pdf Nature Biotechnology]; [http://dl.dropbox.com/u/5389110/nrg2924.pdf Nature Reviews Genetics]
<span style="font-size:115%">Role of bioinspired polymers in determination of pluripotent stem cell fate ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756563/pdf/nihms-132034.pdf PDF])
Sheena Abraham, Nikolai Eroshenko & Raj R. Rao, ''Regenerative Medicine'' 4:561 (2009)

Latest revision as of 13:10, 4 April 2016