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| == Research ==
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| I am building new technologies for basic and applied biology. Long-term research interests include:
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| * human transgene vectors that integrate genes into precise locations on the genome
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| * cheaper, faster, and more accurate gene synthesis
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| * library vs. library screens which quantify the strength of protein-protein and protein-DNA interactions
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| <br>
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| == Publications ==
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| <span style="font-size:115%"> '''Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors''' ([http://dl.dropbox.com/u/5389110/gt2011134a.pdf PDF])</span>
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| <br>
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| Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao, ''Gene Therapy'' 19:469 (2012)
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| * <span style="font-size:85%">[http://www.nature.com/gt/journal/v19/n5/suppinfo/gt2011134s1.html Supplementary Data]
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| <br>
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| <span style="font-size:115%">'''Gene assembly from chip-synthesized oligonucleotides''' ([http://dl.dropbox.com/u/5389110/GeneAssemblyFromChipSynthesizedOligonucleotides%20(1).pdf PDF]) </span>
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| Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church, ''Current Protocols in Chemical Biology'' 4:1 (2012)
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| * <span style="font-size:85%"> Gene Assembly by Subpool PCR (GASP) oligo design [https://bitbucket.org/skosuri/gasp/src/364d21def3ec/cleaned-up-scripts/gaspserver/ scripts] and [http://synbiosis.med.harvard.edu:8080/gaspserver.rpy server] </span>
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| <br>
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| <span style="font-size:115%">'''Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips''' ([http://dl.dropbox.com/u/5389110/nbt.1716.pdf PDF])</span>
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| Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church, ''Nature Biotechnology'' 28:1295 (2010)
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| * <span style="font-size:85%">[http://www.nature.com/nbt/journal/v28/n12/extref/nbt.1716-S1.pdf Supplementary Text and Figures] (.pdf)
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| * <span style="font-size:85%">[http://www.nature.com/nbt/journal/v28/n12/extref/nbt.1716-S2.xls Supplementary Sequences] (.xls)
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| * <span style="font-size:85%">Commentary: [http://dl.dropbox.com/u/5389110/nbt.1723.pdf Nature Biotechnology]; [http://dl.dropbox.com/u/5389110/nrg2924.pdf Nature Reviews Genetics]]
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| <br>
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| <span style="font-size:115%">'''Role of bioinspired polymers in determination of pluripotent stem cell fate''' ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756563/pdf/nihms-132034.pdf PDF]) </span>
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| Sheena Abraham, Nikolai Eroshenko & Raj R. Rao, ''Regenerative Medicine'' 4:561 (2009)
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| ==Patents==
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| Orthogonal amplification and assembly of nucleic acid sequences PCT/US2011/057075 [http://patentscope.wipo.int/search/en/detail.jsf?docId=WO2012154201&recNum=228&docAn=US2011057075&queryString=EN_ALLTXT:(quantum)&maxRec=55226 pending]
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| ==Labs==
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| [http://arep.med.harvard.edu/ George Church] (2009-now)
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| [http://wyss.harvard.edu/ Wyss Institute] (2009-2011)
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| [http://www.engineering.vcu.edu/rao-lab/New_Rao_Site/Raohome.php Raj Rao] (2006-2008)
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| [http://plaza.ufl.edu/curtis.taylor/Florida_Nanoscale_Manufacturing/Home.html Curtis Taylor] (2006-2007)
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| ==Education==
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| [http://seas.harvard.edu/ Harvard University] (2008-now) PhD candidate, Bioengineering
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| [http://www.egr.vcu.edu/Page.aspx?id=93 Virginia Commonwealth University] (2005-2008) BS, Biomedical Engineering
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| ==Contact==
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| eroshenkona AT gmail
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| Nikolai Eroshenko <br>
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| 77 Ave. Louis Pasteur<br>
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| NRB 238 <br>
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| Boston, MA 02115
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