User:Nikolai Eroshenko

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== Research ==
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I am building new technologies for basic and applied biology. Long-term research interests include:
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* fully synthetic (i.e. non-immunogenic) gene therapy vectors that integrate genes into a precise location on the genome
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* cheaper, faster, and more accurate gene synthesis
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* library vs. library screens which quantify the strength of protein-protein and protein-DNA interactions
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== Publications ==
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====Gene assembly from chip-synthesized oligonucleotides (http://dl.dropbox.com/u/5389110/GeneAssemblyFromChipSynthesizedOligonucleotides%20(1).pdf PDF)====
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Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church, ''Current Protocols in Chemical Biology'' 4 (2012)
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* <span style="font-size:85%"> Gene Assembly by Subpool PCR (GASP) oligo design [https://bitbucket.org/skosuri/gasp/src/364d21def3ec/cleaned-up-scripts/gaspserver/ scripts] and [http://synbiosis.med.harvard.edu:8080/gaspserver.rpy server] </span>
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====Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors (PDF)====

Revision as of 09:29, 3 June 2012

Contents

Research

I am building new technologies for basic and applied biology. Long-term research interests include:

  • fully synthetic (i.e. non-immunogenic) gene therapy vectors that integrate genes into a precise location on the genome
  • cheaper, faster, and more accurate gene synthesis
  • library vs. library screens which quantify the strength of protein-protein and protein-DNA interactions

Publications

Gene assembly from chip-synthesized oligonucleotides (http://dl.dropbox.com/u/5389110/GeneAssemblyFromChipSynthesizedOligonucleotides%20(1).pdf PDF)

Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church, Current Protocols in Chemical Biology 4 (2012)

  • Gene Assembly by Subpool PCR (GASP) oligo design scripts and server

Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors (PDF)

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