User:Nikolai Eroshenko
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== Publications == | == Publications == | ||
| - | = | + | <span style="font-size:115%">'''Gene assembly from chip-synthesized oligonucleotides''' ([http://dl.dropbox.com/u/5389110/GeneAssemblyFromChipSynthesizedOligonucleotides%20(1).pdf PDF]) |
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Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church, ''Current Protocols in Chemical Biology'' 4 (2012) | Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church, ''Current Protocols in Chemical Biology'' 4 (2012) | ||
* <span style="font-size:85%"> Gene Assembly by Subpool PCR (GASP) oligo design [https://bitbucket.org/skosuri/gasp/src/364d21def3ec/cleaned-up-scripts/gaspserver/ scripts] and [http://synbiosis.med.harvard.edu:8080/gaspserver.rpy server] </span> | * <span style="font-size:85%"> Gene Assembly by Subpool PCR (GASP) oligo design [https://bitbucket.org/skosuri/gasp/src/364d21def3ec/cleaned-up-scripts/gaspserver/ scripts] and [http://synbiosis.med.harvard.edu:8080/gaspserver.rpy server] </span> | ||
| - | = | + | <span style="font-size:115%"> Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors ([http://dl.dropbox.com/u/5389110/gt2011134a.pdf PDF]) |
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Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao, ''Gene Therapy'' AOP (2011) | Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao, ''Gene Therapy'' AOP (2011) | ||
| - | = | + | <span style="font-size:115%">Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips ([http://dl.dropbox.com/u/5389110/nbt.1716.pdf PDF]) |
Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church, ''Nature Biotechnology'' 28:1295 (2010) | Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church, ''Nature Biotechnology'' 28:1295 (2010) | ||
| - | * <span style="font-size:85%"> [http://www.nature.com/nbt/journal/v28/n12/extref/nbt.1716-S1.pdf Supplementary Text and Figures] (.pdf) | + | * <span style="font-size:85%">[http://www.nature.com/nbt/journal/v28/n12/extref/nbt.1716-S1.pdf Supplementary Text and Figures] (.pdf) |
| - | * [http://www.nature.com/nbt/journal/v28/n12/extref/nbt.1716-S2.xls Supplementary Sequences] (.xls) | + | * <span style="font-size:85%">[http://www.nature.com/nbt/journal/v28/n12/extref/nbt.1716-S2.xls Supplementary Sequences] (.xls) |
| - | * [http://dl.dropbox.com/u/5389110/Science-2010-News%20Focus-1605-7.pdf Runner-up for Science 2011 Breakthrough of the Year] | + | * <span style="font-size:85%">[http://dl.dropbox.com/u/5389110/Science-2010-News%20Focus-1605-7.pdf Runner-up for Science 2011 Breakthrough of the Year] |
| - | * Commentary: [http://dl.dropbox.com/u/5389110/nbt.1723.pdf Nature Biotechnology]; [http://dl.dropbox.com/u/5389110/nrg2924.pdf Nature Reviews Genetics] < | + | * <span style="font-size:85%">Commentary: [http://dl.dropbox.com/u/5389110/nbt.1723.pdf Nature Biotechnology]; [http://dl.dropbox.com/u/5389110/nrg2924.pdf Nature Reviews Genetics] |
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| + | <span style="font-size:115%">Role of bioinspired polymers in determination of pluripotent stem cell fate ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756563/pdf/nihms-132034.pdf PDF]) | ||
| + | Sheena Abraham, Nikolai Eroshenko & Raj R. Rao, ''Regenerative Medicine'' 4:561 (2009) | ||
Revision as of 09:42, 3 June 2012
Research
I am building new technologies for basic and applied biology. Long-term research interests include:
- fully synthetic (i.e. non-immunogenic) gene therapy vectors that integrate genes into a precise location on the genome
- cheaper, faster, and more accurate gene synthesis
- library vs. library screens which quantify the strength of protein-protein and protein-DNA interactions
Publications
Gene assembly from chip-synthesized oligonucleotides (PDF)
Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church, Current Protocols in Chemical Biology 4 (2012)
Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors (PDF)
Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao, Gene Therapy AOP (2011)
Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips (PDF) Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church, Nature Biotechnology 28:1295 (2010)
- Supplementary Text and Figures (.pdf)
- Supplementary Sequences (.xls)
- Runner-up for Science 2011 Breakthrough of the Year
- Commentary: Nature Biotechnology; Nature Reviews Genetics
Role of bioinspired polymers in determination of pluripotent stem cell fate (PDF) Sheena Abraham, Nikolai Eroshenko & Raj R. Rao, Regenerative Medicine 4:561 (2009)
