User:Nikolai Eroshenko: Difference between revisions
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== Publications == | == Publications == | ||
<span style="font-size:115%">''' | <span style="font-size:115%"> '''Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors''' ([http://dl.dropbox.com/u/5389110/gt2011134a.pdf PDF])</span> | ||
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Nikolai Eroshenko | Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao, ''Gene Therapy'' AOP 19:469 (2012) | ||
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<span style="font-size:115%"> ''' | <span style="font-size:115%">'''Gene assembly from chip-synthesized oligonucleotides''' ([http://dl.dropbox.com/u/5389110/GeneAssemblyFromChipSynthesizedOligonucleotides%20(1).pdf PDF]) </span> | ||
Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church, ''Current Protocols in Chemical Biology'' 4 (2012) | |||
* <span style="font-size:85%"> Gene Assembly by Subpool PCR (GASP) oligo design [https://bitbucket.org/skosuri/gasp/src/364d21def3ec/cleaned-up-scripts/gaspserver/ scripts] and [http://synbiosis.med.harvard.edu:8080/gaspserver.rpy server] </span> | |||
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Revision as of 14:51, 6 December 2012
Research
I am building new technologies for basic and applied biology. Long-term research interests include:
- fully synthetic (i.e. non-immunogenic) gene therapy vectors that integrate genes into a precise location on the genome
- cheaper, faster, and more accurate gene synthesis
- library vs. library screens which quantify the strength of protein-protein and protein-DNA interactions
Publications
Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors (PDF)
Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao, Gene Therapy AOP 19:469 (2012)
Gene assembly from chip-synthesized oligonucleotides (PDF)
Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church, Current Protocols in Chemical Biology 4 (2012)
Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips (PDF)
Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church, Nature Biotechnology 28:1295 (2010)
- Supplementary Text and Figures (.pdf)
- Supplementary Sequences (.xls)
- Commentary: Nature Biotechnology; Nature Reviews Genetics]
Role of bioinspired polymers in determination of pluripotent stem cell fate (PDF)
Sheena Abraham, Nikolai Eroshenko & Raj R. Rao, Regenerative Medicine 4:561 (2009)
Labs
George Church (2009-now)
Wyss Institute (2009-2011)
Raj Rao (2006-2008)
Curtis Taylor (2006-2007)
Education
Harvard University (2008-now) PhD candidate, Bioengineering
Virginia Commonwealth University (2005-2008) BS, Biomedical Engineering
Contact
eroshenkona AT gmail
Nikolai Eroshenko
77 Ave. Louis Pasteur
NRB 238
Boston, MA 02115