User:Nikolai Eroshenko

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== Publications ==
== Publications ==
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<span style="font-size:115%"> '''Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors'''</span>
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<span style="font-size:115%"> Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors</span>
<br>
<br>
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Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao, ''Gene Therapy'' 19:469 (2012)  
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<span style="font-size:85%">Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao, ''Gene Therapy'' 19:469 (2012)</span>
* <span style="font-size:85%">[http://www.nature.com/gt/journal/v19/n5/suppinfo/gt2011134s1.html Supplementary Data]  
* <span style="font-size:85%">[http://www.nature.com/gt/journal/v19/n5/suppinfo/gt2011134s1.html Supplementary Data]  
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<br>
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<span style="font-size:115%">Gene assembly from chip-synthesized oligonucleotides ([http://dl.dropbox.com/u/5389110/GeneAssemblyFromChipSynthesizedOligonucleotides%20(1).pdf PDF]) </span>
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<span style="font-size:115%">'''Gene assembly from chip-synthesized oligonucleotides''' ([http://dl.dropbox.com/u/5389110/GeneAssemblyFromChipSynthesizedOligonucleotides%20(1).pdf PDF]) </span>
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<span style="font-size:85%">Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church, ''Current Protocols in Chemical Biology'' 4:1 (2012)</span>
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Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church, ''Current Protocols in Chemical Biology'' 4:1 (2012)
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* <span style="font-size:85%"> Gene Assembly by Subpool PCR (GASP) oligo design [https://bitbucket.org/skosuri/gasp/src/364d21def3ec/cleaned-up-scripts/gaspserver/ scripts] and [http://synbiosis.med.harvard.edu:8080/gaspserver.rpy server] </span>
* <span style="font-size:85%"> Gene Assembly by Subpool PCR (GASP) oligo design [https://bitbucket.org/skosuri/gasp/src/364d21def3ec/cleaned-up-scripts/gaspserver/ scripts] and [http://synbiosis.med.harvard.edu:8080/gaspserver.rpy server] </span>
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<span style="font-size:115%">'''Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips''' ([http://dl.dropbox.com/u/5389110/nbt.1716.pdf PDF])</span>
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<span style="font-size:115%">Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips'([http://dl.dropbox.com/u/5389110/nbt.1716.pdf PDF])</span>
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Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church, ''Nature Biotechnology'' 28:1295 (2010)  
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<span style="font-size:85%">Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church, ''Nature Biotechnology'' 28:1295 (2010)</span>
* <span style="font-size:85%">[http://www.nature.com/nbt/journal/v28/n12/extref/nbt.1716-S1.pdf Supplementary Text and Figures] (.pdf)
* <span style="font-size:85%">[http://www.nature.com/nbt/journal/v28/n12/extref/nbt.1716-S1.pdf Supplementary Text and Figures] (.pdf)
* <span style="font-size:85%">[http://www.nature.com/nbt/journal/v28/n12/extref/nbt.1716-S2.xls Supplementary Sequences] (.xls)
* <span style="font-size:85%">[http://www.nature.com/nbt/journal/v28/n12/extref/nbt.1716-S2.xls Supplementary Sequences] (.xls)
* <span style="font-size:85%">Commentary: [http://dl.dropbox.com/u/5389110/nbt.1723.pdf Nature Biotechnology]; [http://dl.dropbox.com/u/5389110/nrg2924.pdf Nature Reviews Genetics]]
* <span style="font-size:85%">Commentary: [http://dl.dropbox.com/u/5389110/nbt.1723.pdf Nature Biotechnology]; [http://dl.dropbox.com/u/5389110/nrg2924.pdf Nature Reviews Genetics]]
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<br>
 
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<span style="font-size:115%">'''Role of bioinspired polymers in determination of pluripotent stem cell fate''' ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756563/pdf/nihms-132034.pdf PDF]) </span>
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<span style="font-size:115%">Role of bioinspired polymers in determination of pluripotent stem cell fate ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756563/pdf/nihms-132034.pdf PDF]) </span>
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Sheena Abraham, Nikolai Eroshenko & Raj R. Rao, ''Regenerative Medicine'' 4:561 (2009)
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<span style="font-size:85%">Sheena Abraham, Nikolai Eroshenko & Raj R. Rao, ''Regenerative Medicine'' 4:561 (2009)</span>
==Patents==
==Patents==

Revision as of 10:32, 20 February 2013

Contents

Research

I am building new technologies for basic and applied biology. Long-term research interests include:

  • human transgene vectors that integrate genes into precise locations on the genome
  • cheaper, faster, and more accurate gene synthesis
  • library vs. library screens which quantify the strength of protein-protein and protein-DNA interactions


Publications

Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors

Shilpa Iyer, Ena Xiao, Khaled Alsayegh, Nikolai Eroshenko, Marion J. Riggs, James P. Bennett. Jr & Raj R. Rao, Gene Therapy 19:469 (2012)

Gene assembly from chip-synthesized oligonucleotides (PDF)

Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church, Current Protocols in Chemical Biology 4:1 (2012)

  • Gene Assembly by Subpool PCR (GASP) oligo design scripts and server

Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips'(PDF)

Sriram Kosuri*, Nikolai Eroshenko*, Emily LeProust, Michael Super, Jeffrey Way, Jin Billy Li & George Church, Nature Biotechnology 28:1295 (2010)

Role of bioinspired polymers in determination of pluripotent stem cell fate (PDF)

Sheena Abraham, Nikolai Eroshenko & Raj R. Rao, Regenerative Medicine 4:561 (2009)

Patents

Orthogonal amplification and assembly of nucleic acid sequences, PCT/US2011/057075, pending

Labs

George Church (2009-now)

Wyss Institute (2009-2011)

Raj Rao (2006-2008)

Curtis Taylor (2006-2007)

Education

Harvard University (2008-now) PhD candidate, Bioengineering

Virginia Commonwealth University (2005-2008) BS, Biomedical Engineering

Contact

eroshenkona AT gmail


Nikolai Eroshenko
77 Ave. Louis Pasteur
NRB 238
Boston, MA 02115

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