User:Nikolai Eroshenko

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Research

I am building new technologies for basic and applied biology. Long-term research interests include:

  • fully synthetic (i.e. non-immunogenic) gene therapy vectors that integrate genes into a precise location on the genome
  • cheaper, faster, and more accurate gene synthesis
  • library vs. library screens which quantify the strength of protein-protein and protein-DNA interactions

Publications

Gene assembly from chip-synthesized oligonucleotides (http://dl.dropbox.com/u/5389110/GeneAssemblyFromChipSynthesizedOligonucleotides%20(1).pdf PDF)

Nikolai Eroshenko*, Sriram Kosuri*, Adam H. Marblestone, Nicholas Conway, George Church, Current Protocols in Chemical Biology 4 (2012)

  • Gene Assembly by Subpool PCR (GASP) oligo design scripts and server

Mitochondrial gene replacement in human pluripotent stem cell derived neural progenitors (PDF)

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