User:Nikolai Slavov

Contact Info

Nikolai Slavov

• Nikolai Slavov
• Massachusetts Institute of Technology, 68-359
• 77 Massachusetts Ave Cambridge, MA 02139
• http://mit.edu/nslavov/www/
• http://alum.mit.edu/www/nslavov
• Email me through OpenWetWare

Education

• 2010, PhD, Botstein Lab[1], Princeton University
• 2006, MS, Princeton University
• 2004, BS, Massachusetts Institute of Technology

Research interests

1. I am interested in the dynamics of cell growth, metabolic and regulatory processes during the eukaryotic cell division cycle (CDC), across a wide range of CDC periods. In particular, I study how the growth rate signal - which is function of the availability of nutrients and growth factors - regulates the switch between respiration and fermentation and changes the duration of phases of the cell growth and division cycles.

Publications

1. Slavov N, Macinskas J, Caudy A, and Botstein D. Metabolic cycling without cell division cycling in respiring yeast. Proc Natl Acad Sci U S A. 2011 Nov 22;108(47):19090-5. DOI:10.1073/pnas.1116998108 |
2. Slavov N and Botstein D. Coupling among growth rate response, metabolic cycle, and cell division cycle in yeast. Mol Biol Cell. 2011 Jun 15;22(12):1997-2009. DOI:10.1091/mbc.E11-02-0132 |

   We discovered that the relative durations of the phases of the yeast metabolic cycle change with the growth rate. These changes can explain mechanistically the transcriptional growth-rate responses of all yeast genes (25% of the genome) that we find to be the same across all studied nutrient limitations in either ethanol or glucose carbon source.

3. Silverman SJ, Petti AA, Slavov N, Parsons L, Briehof R, Thiberge SY, Zenklusen D, Gandhi SJ, Larson DR, Singer RH, and Botstein D. Metabolic cycling in single yeast cells from unsynchronized steady-state populations limited on glucose or phosphate. Proc Natl Acad Sci U S A. 2010 Apr 13;107(15):6946-51. DOI:10.1073/pnas.1002422107 |
4. Slavov N and Dawson KA. Correlation signature of the macroscopic states of the gene regulatory network in cancer. Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4079-84. DOI:10.1073/pnas.0810803106 |

Protocols

• Phosphate-Limited Medium with Ethanol as a Sole Source of Carbon and Energy[2]
• Glucose-Limited Medium[3]