User:Nishant M. Bhat/Protocols: Difference between revisions

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[[Nmbhat:Antibiotic_cultures]]
[[Nmbhat:Antibiotic_cultures]]
*dNTP dilution:
**108 H2O + 3 of each NTP (100mM)

Revision as of 08:06, 10 July 2007

Transforming chemically competent cells

Colony PCR (Endy)

Pouring LB plates (Endy)

Restriction Digests (Knight)

Streaking plates

3-way ligation (Bhat) (Adapted from the Endy protocol)

Making competent cells

Endy:Agarose_gel

Nmbhat:Antibiotic_cultures


  • dNTP dilution:
    • 108 H2O + 3 of each NTP (100mM)
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