User:Nishant M. Bhat/Protocols: Difference between revisions
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[[Transforming chemically competent cells]] | [[Transforming chemically competent cells]] | ||
[[Endy:Colony PCR]] | [[Endy:Colony PCR|Colony PCR (Endy)]] | ||
[[Endy:Pouring LB plates from prepped media]] | [[Endy:Pouring LB plates from prepped media|Pouring LB plates (Endy)]] | ||
[[Knight:Restriction_Digest]] | [[Knight:Restriction_Digest|Restriction Digests (Knight)]] | ||
[[Streak_plates|Streaking plates]] | |||
[[Nmbhat:3-way ligation|3-way ligation (Bhat)]] | |||
(Adapted from [[Endy:DNA_ligation_using_T4_DNA_ligase|the Endy protocol]]) | |||
[[Preparing_chemically_competent_cells|Making competent cells]] | |||
[[Endy:Agarose_gel]] | |||
[[Nmbhat:Antibiotic_cultures]] | |||
[[Endy:PCR_Supermix]] | |||
*dNTP dilution: | |||
**108 H2O + 3 of each NTP (100mM) | |||
Latest revision as of 11:25, 23 July 2007
Transforming chemically competent cells
3-way ligation (Bhat) (Adapted from the Endy protocol)
- dNTP dilution:
- 108 H2O + 3 of each NTP (100mM)
