User:Nkuldell/SAGA swap pt 2

From OpenWetWare

Jump to: navigation, search

Reagents

BBa_Y00073 [[1]]
had DNA 2.0 codon-optimize S. pombe gene SPCC126.04c [[2]] including biobrick ends for expression of ORF in S. cerevisiae This subunit is homolog of S. cerevisiae SGF73 [[3]] subunit of SAGA and deletion of this subunit in S. cerevisiae results in viable cells but no induction of PAU genes under hypoxic conditions.

alignment of S. cerevisiae Sgf73p with S. pombe SPCC126.04c shows alignments btw S.cerevisiae aa 68 to aa 398 with S. pombe seq aa 74 to 318. Sgf73 has C-term region without homology to smaller S.pombe protein. Image:Sc Sp Sgf73.pdfand Image:Sp Sc Sgf73pseq.pdf
Quite different is the comparison of S. cerevisiae Sgf29 to S. pombe SPBC1921.07c which are proteins of approx the same length (259aa for S.c. vs 244aa for S.p) but which align only at the C-term half. Image:Sp Sc Sgf29p .pdf

Personal tools