User:Nkuldell/SAGA vs Swi/Snf: Difference between revisions

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| 3.999 kb/1332aa, Chr. II, <br> viable  
| 3.999 kb/1332aa, Chr. II, <br> viable  
| FY963
| FY963
| MAT(A)  ura3-52 leu2d1 his4-917d <br> <b> spt7::LEU2 </b>
| MAT(A)  ura3-52 his4-917d leu2d1 <br> <b> spt7::LEU2 </b>
|-
|-
| Spt8 [[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=Spt8]]   
| Spt8 [[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=Spt8]]   
| 1.809 kb/602aa, Chr. XII, <br> viable
| 1.809 kb/602aa, Chr. XII, <br> viable
| FY50
| FY50
| MAT(A)  ura3-52 leu2d1 his4-917d trp1d63 <br> <b>spt8d-302::LEU2 </b>
| MAT(A)  ura3-52 his4-917d leu2d1 trp1d63 <br> <b>spt8d-302::LEU2 </b>
|-   
|-   
| Spt20 (aka Ada5) [[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=SPT20]]   
| Spt20 (aka Ada5) [[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=SPT20]]   
| 1.815 kb/604aa, Chr. XV, <br> viable
| 1.815 kb/604aa, Chr. XV, <br> viable
| FY1098
| FY1098
| MAT(A) ura3-52 leu2d1 his3d200 <br> <b>spt20d100::URA3 </b>
| MAT(A) ura3-52 his3d200 leu2d1 <br> <b>spt20d100::URA3 </b>
|}
|}
{| border="1"  
{| border="1"  
! TAF subunits, cerevisiae </i> 
! TAF subunits  
! size, chromosome, null p-type  
! size, chromosome, null p-type  
|-   
|-   
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|-   
|-   
| Sgf73 [[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=sgf73]]   
| Sgf73 [[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=sgf73]]   
| 1.974 kb/657aa, Chr. VII , viable  
| 1.974 kb/657aa, Chr. VII , <br> viable  
|
|
|  
|  
|-
|-
| Sgf29 [[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=sgf29]]   
| Sgf29 [[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=sgf29]]   
| 0.779 kb/259aa, Chr. III, viable
| 0.779 kb/259aa, Chr. III, <br> viable
|
| FY2474
|
| MAT(A) ura3-52 his4-917d leu2D1 lys2-173R2 arg4-12 <br> <b> sgf29D0::KanMX </b>
|-
|-
| Sgf11 [[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=sgf11]]   
| Sgf11 [[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=sgf11]]   
| 0.3 kb/99aa, Chr.XVI, viable
| 0.3 kb/99aa, Chr.XVI, <br> viable
|
|
|   
|   
|-
|-
| Ubp8 [[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=ubp8]]   
| Ubp8 [[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=ubp8]]   
| 1.416 kb/471aa, Chr. XIII, viable  
| 1.416 kb/471aa, Chr. XIII, <br> viable  
|
| FY2473
|  
| MAT(alpha) ura3-52 leu2D1 lys2-173R2 arg4-12 <br> <b>ubp8D0::KanMX </b>
|-   
|-   
| Sus1 [[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=Sus1]]   
| Sus1 [[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=Sus1]]   
| gene with intron, Chr. II, viable   
| gene with intron, Chr. II, <br> viable   
|
|
|
|
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|-
|-
|SWII (aka ADR6, GAM3, LPA1)[[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=swi1]]
|SWII (aka ADR6, GAM3, LPA1)[[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=swi1]]
|3945bp/1315 aa, Chr. XVI, <font color =red> inviable </font color>  
|3945bp/1315 aa, Chr. XVI, <br> <font color =red> inviable </font color>  
|-
|-
|SWI2 (aka GAM1, HAF1, <font color = red> SNF2 </font color>, TYE3)[[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=SWI2]]
|SWI2 (aka GAM1, HAF1, <font color = red> SNF2 </font color>, TYE3)[[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=SWI2]]
|5112bp/1704aa, Chr. XV, viable
|5112bp/1704aa, Chr. XV, <br> viable
|-
|-
|SWI3 (aka TYE2) [[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=swi3]]
|SWI3 (aka TYE2) [[http://db.yeastgenome.org/cgi-bin/locus.pl?locus=swi3]]
|2478bp/826aa, Chr. X, viable
|2478bp/826aa, Chr. X,<br> viable
|}
|}
{| border="1"
{| border="1"

Revision as of 20:42, 31 October 2006

in progress...

SAGA subunits, S. cerevisiae

most strains listed in Martens, Wu, Winston G&D paper 2005 [[1]]
link to FW yeast strain collection [[2]]
Parent strain for 109 deletion analysis: FY2068 = MAT(alpha) ura3-52 his3D200 leu2D1 lys2-128d

Ada subunits size,chromosome,null p-type deletion strain genotype
Ada1 (aka HFI1, SUP110, SRM12, GAN1) [[3]] 1.467 kb/489 aa, Chr. XVI,
viable 		FY1560 MAT(A) ura3-52 his3D200 lys2-173R2
ada1::HIS3
Ada2 (aka SWI8) [[4]] 1.305 kb/434aa, Chr. IV,
viable 		FY1548 MAT(A) ura3-52 his3D200 leu2D1 lys2-128d
ada2::HIS3
Ada3 (aka NGG1, SWI7) [[5]] 2.109 kb/702aa, Chr. IV,
viable 		FY1596 MAT(A) ura3-52 his3D200 his4-912d lys2-173R2
ada3::HIS3
Gcn5 (aka ADA4, SWI9) [[6]] 1.32 kb/439aa, Chr. VII,
viable 		FY1600 MAT(A) ura3-52 leu2D1 his3D200 his4-917d
gcn5::HIS3
Ada5 (aka SPT20) [[7]] 1.815 kb/604aa, Chr. XV,
viable 		FY1098 see spt20::URA3
Spt subunits size, chromosome, null p-type deletion strain genotype
Spt3 [[8]] 1.014 kb/337aa, Chr. IV,
viable 		FY2142 MAT (alpha)
spt3::KanMX
Spt7 (aka GIT2) [[9]] 3.999 kb/1332aa, Chr. II,
viable 		FY963 MAT(A) ura3-52 his4-917d leu2d1
spt7::LEU2
Spt8 [[10]] 1.809 kb/602aa, Chr. XII,
viable 		FY50 MAT(A) ura3-52 his4-917d leu2d1 trp1d63
spt8d-302::LEU2
Spt20 (aka Ada5) [[11]] 1.815 kb/604aa, Chr. XV,
viable 		FY1098 MAT(A) ura3-52 his3d200 leu2d1
spt20d100::URA3
TAF subunits size, chromosome, null p-type
TAF5 (aka TAF90) [[12]] 2.397 kb/798aa, Chr. II, inviable
TAF6 (aka TAF60) [[13]] 1.551 kb/516aa, Chr. VII, inviable
TAF9 (aka TAF17) [[14]] 0.474 kb/157aa, Chr. XIII, inviable
TAF10 (aka TAF23, TAF25) [[15]] 0.621 kb/206aa, Chr. IV, inviable
TAF12 (aka TAF61, TAF68) [[16]] 1.620 kb/539aa, Chr. IV, inviable
Tra1 subunit size, chromosome, null p-type
Tra1 [[17]] 11.235 kb/3744aa, Chr. VIII, inviable
other subunits size, chromosome, null p-type deletion strain genotype
Sgf73 [[18]] 1.974 kb/657aa, Chr. VII ,
viable
Sgf29 [[19]] 0.779 kb/259aa, Chr. III,
viable 		FY2474 MAT(A) ura3-52 his4-917d leu2D1 lys2-173R2 arg4-12
sgf29D0::KanMX
Sgf11 [[20]] 0.3 kb/99aa, Chr.XVI,
viable
Ubp8 [[21]] 1.416 kb/471aa, Chr. XIII,
viable 		FY2473 MAT(alpha) ura3-52 leu2D1 lys2-173R2 arg4-12
ubp8D0::KanMX
Sus1 [[22]] gene with intron, Chr. II,
viable

SWI/SNF subunits

SWI subunits size,chromosome,null p-type
SWII (aka ADR6, GAM3, LPA1)[[23]] 3945bp/1315 aa, Chr. XVI,
inviable
SWI2 (aka GAM1, HAF1, SNF2 , TYE3)[[24]] 5112bp/1704aa, Chr. XV,
viable
SWI3 (aka TYE2) [[25]] 2478bp/826aa, Chr. X,
viable
SNF subunits size, chromosome, null p-type
SNF5
SNF6
SNF11
SWP subunits size, chromosome, null p-type
SWP82
SWP73
SWP59
SWP61
SWP29
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