User:Nkuldell/mtDNA pt3

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(New page: ===Overall goal=== Make a tool to regulate gene expression of any mt gene. Tool would have two-parts and be nuclear-encoded. *Part 1: Rnt1p (RNaseIII enzyme), targetted to mt using sig se...)
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*Part 1: Rnt1p (RNaseIII enzyme), targetted to mt using sig sequence from HEM1 or COX4.
*Part 1: Rnt1p (RNaseIII enzyme), targetted to mt using sig sequence from HEM1 or COX4.
*Part 2: Guide RNAs, targetted to mt using lysing-tRNA-CUU (tRK1) import system.
*Part 2: Guide RNAs, targetted to mt using lysing-tRNA-CUU (tRK1) import system.
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Summary of natural transcriptional control elements [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=2251275&ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Biswas in PNAS 1990 87:9338] and [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=2229061&ordinalpos=2&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Biswas and Getz in J Biol Chem 1990 265:19053]
===Part 1: Rnt1p===
===Part 1: Rnt1p===

Revision as of 16:39, 13 June 2007

Contents

Overall goal

Make a tool to regulate gene expression of any mt gene. Tool would have two-parts and be nuclear-encoded.

  • Part 1: Rnt1p (RNaseIII enzyme), targetted to mt using sig sequence from HEM1 or COX4.
  • Part 2: Guide RNAs, targetted to mt using lysing-tRNA-CUU (tRK1) import system.

Summary of natural transcriptional control elements Biswas in PNAS 1990 87:9338 and Biswas and Getz in J Biol Chem 1990 265:19053

Part 1: Rnt1p

Gen'l info about RNases

S. cerevisiae RNAases

Rnt1p

Part 2: Guide RNAs

Structural requirements for guide RNAs

Cell components needed for moving guide RNA to mt

  • piggy back

Experimental checkpoints for regulated expression

Part 1: Rnt1p in mt

    1. add tag to gene to follow localization of protein product
    2. microarray induced/uninduced to look for effect of RNase in mt.

Applications for mt gene regulation, in the unlikely case that this system works

Personal tools