User:Nkuldell/other rewrites

• stepwise move centromere from one end of chromosome to other
• progressively larger deletions from telomere to first essential gene on each chromosome
• clustering highly expressed genes on one arm, poorly expressed genes on other
• clustering related genes into "operon"
• multiple copies of yeast mating locus
• replace S. cerevisiae genes for single complex (e.g. SAGA) with homologs from other yeast starting with pombe
• in vivo reporter for transciption elongation: functional RNA?
• randomize sigma 4.2 region of TFG2 and screen library for rifS and FLO:HIS mutants
• check Drew's T7.1 for revertants that increase plaque size back to normal.
• biobrick assembly for yeast parts (look at Pam's yeast parts first!)...ideally would use homologous recomb vs restriction sites