User:Nkuldell:M13 redesign
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[[M13 redesign, expt'l details]] | [[M13 redesign, expt'l details]] | ||
==Due Diligence== | ==Due Diligence== | ||
| - | |||
===M13 phage proteins=== | ===M13 phage proteins=== | ||
| - | [[Image:Macintosh HD-Users-nkuldell-Desktop-M13K07 plasmid-phageproteins.jpeg|thumb|left| phage proteins]] | + | [[Image:Macintosh HD-Users-nkuldell-Desktop-M13K07 plasmid-phageproteins.jpeg|thumb|left|500px| phage proteins]] |
| - | NCBI link to seq: [[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=56713234]] <br> | + | [[Image:Macintosh HD-Users-nkuldell-Desktop-M13K07 plasmid-phageparticle.jpeg|thumb|left| phage particle cartoon]] <br> <br> <br> |
| - | Source: Phage Display of Peptides and Proteins: a laboratory manual. Kay, Winter, McCafferty. Ch 1. [[http://www.amazon.com/Phage-Display-Peptides-Proteins-Laboratory/dp/0124023800]]<br> | + | *NCBI link to seq: [[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=56713234]] <br> |
| + | *Source: Phage Display of Peptides and Proteins: a laboratory manual. Kay, Winter, McCafferty. Ch 1. [[http://www.amazon.com/Phage-Display-Peptides-Proteins-Laboratory/dp/0124023800]]<br> | ||
{| border="1" | {| border="1" | ||
! protein | ! protein | ||
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|} | |} | ||
| - | + | ===Life Cycle of the Phage=== | |
| + | [[Image:Macintosh HD-Users-nkuldell-Desktop-M13K07 plasmid-M13lifecycle.jpeg|thumb|left|350px| cartoon depiction for life cycle of M13]] | ||
| + | [[Image:Macintosh HD-Users-nkuldell-Desktop-M13K07 plasmid-lifecycle key.jpeg|thumb|left|500px| key]] | ||
==Makeover ideas== | ==Makeover ideas== | ||
===phage or phage coat rewrite=== | ===phage or phage coat rewrite=== | ||
===separate 8, 9, 3=== | ===separate 8, 9, 3=== | ||
===two copies of 8=== | ===two copies of 8=== | ||
Current revision
Contents |
Due Diligence
M13 phage proteins
- NCBI link to seq: [[1]]
- Source: Phage Display of Peptides and Proteins: a laboratory manual. Kay, Winter, McCafferty. Ch 1. [[2]]
| protein | aa (mature) | function | notes |
|---|---|---|---|
| I | 348 | assembly | includes gXI within, the two form structure in cytoplasmic membrane for phage maturation |
| II | 410 | replication of DNA + strand | includes gX within |
| III | 406 | phage tail protein (5 copies) | tag at N-term |
| IV | 405 | assembly | organizes to pore in OM |
| V | 87 | binds ssDNA | recognizes DNA PS |
| VI | 112 | phage tail protein (5 copies) | packages with pIII |
| VII | 33 | phage head protein (5 copies) | packages with p9 |
| VIII | 50 | phage coat protein (2700 copies) | seg seq cleaved, matured form at aa 23 = clones to N-term, "Petrenko" site [[3]] |
| IX | 32 | phage head protein (5 copies) | stop codon overlaps start of gVIII |
| X | 111 | DNA replication | internal reinitiation inside gII |
| XI | 108 | assembly | translation reinitiation inside gI |
Life Cycle of the Phage
