User:Nkuldell:M13 redesign

Due Diligence

M13 phage proteins

NCBI link to seq: [[1]]
Source: Phage Display of Peptides and Proteins: a laboratory manual. Kay, Winter, McCafferty. Ch 1. [[2]]

protein	aa (mature)	function	notes
I	348	assembly
II	410	replication of DNA + strand
III	406	phage tail protein (5 copies)
IV	405	assembly
V	87	binds ssDNA
VI	112	phage tail protein (5 copies)
VII	33	phage head protein (5 copies)
VIII	50	phage coat protein (2700 copies)
IX	32	phage head protein (5 copies)
X	111	DNA replication
XI	108	assembly

Vectors for M13 protein expression

• M13KE [[3]]
• pUC118 [[4]] or 119 [[5]]
• M13K07 (helper phage)[[6]]