User:Nkuldell:M13 redesign

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Contents

Due Diligence

Life Cycle of the Phage

M13 phage proteins

NCBI link to seq: [[1]]
Source: Phage Display of Peptides and Proteins: a laboratory manual. Kay, Winter, McCafferty. Ch 1. [[2]]

protein aa (mature) function notes
I 348 assembly
II 410 replication of DNA + strand
III 406 phage tail protein (5 copies)
IV 405 assembly
V 87 binds ssDNA
VI 112 phage tail protein (5 copies)
VII 33 phage head protein (5 copies)
VIII 50 phage coat protein (2700 copies)
IX 32 phage head protein (5 copies)
X 111 DNA replication
XI 108 assembly

Vectors for M13 protein expression

  • M13KE [[3]]
  • pUC118 [[4]] or 119 [[5]]
  • M13K07 (helper phage)[[6]]

Makeover ideas

phage or phage coat rewrite

separate 8, 9, 3

two copies of 8

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