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Due Diligence
Life Cycle of the Phage
M13 phage proteins
NCBI link to seq: [[1]]
Source: Phage Display of Peptides and Proteins: a laboratory manual. Kay, Winter, McCafferty. Ch 1. [[2]]
| protein
| aa (mature)
| function
| notes
|
| I
| 348
| assembly
|
|
| II
| 410
| replication of DNA + strand
|
|
| III
| 406
| phage tail protein (5 copies)
|
|
| IV
| 405
| assembly
|
|
| V
| 87
| binds ssDNA
|
|
| VI
| 112
| phage tail protein (5 copies)
|
|
| VII
| 33
| phage head protein (5 copies)
|
|
| VIII
| 50
| phage coat protein (2700 copies)
|
|
| IX
| 32
| phage head protein (5 copies)
|
|
| X
| 111
| DNA replication
|
|
| XI
| 108
| assembly
|
|
Vectors for M13 protein expression
- M13KE [[3]]
- pUC118 [[4]] or 119 [[5]]
- M13K07 (helper phage)[[6]]
Makeover ideas
phage or phage coat rewrite
separate 8, 9, 3
two copies of 8
