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M13 redesign, expt'l details
Due Diligence
M13 phage proteins
- NCBI link to seq: [[1]]
- Source: Phage Display of Peptides and Proteins: a laboratory manual. Kay, Winter, McCafferty. Ch 1. [[2]]
| protein
| aa (mature)
| function
| notes
|
| I
| 348
| assembly
| includes gXI within, the two form structure in cytoplasmic membrane for phage maturation
|
| II
| 410
| replication of DNA + strand
| includes gX within
|
| III
| 406
| phage tail protein (5 copies)
| tag at N-term
|
| IV
| 405
| assembly
| organizes to pore in OM
|
| V
| 87
| binds ssDNA
| recognizes DNA PS
|
| VI
| 112
| phage tail protein (5 copies)
| packages with pIII
|
| VII
| 33
| phage head protein (5 copies)
| packages with p9
|
| VIII
| 50
| phage coat protein (2700 copies)
| seg seq cleaved, matured form at aa 23 = clones to N-term, "Petrenko" site [[3]]
|
| IX
| 32
| phage head protein (5 copies)
| stop codon overlaps start of gVIII
|
| X
| 111
| DNA replication
| internal reinitiation inside gII
|
| XI
| 108
| assembly
| translation reinitiation inside gI
|
Life Cycle of the Phage
cartoon depiction for life cycle of M13
Makeover ideas
phage or phage coat rewrite
separate 8, 9, 3
two copies of 8
