User:Noah Benjamin/Notebook/Experimental Biological Chem/2011/09/20: Difference between revisions
(Autocreate 2011/09/20 Entry for User:Noah_Benjamin/Notebook/Experimental_Biological_Chem) |
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==Description== | ==Description== | ||
The base pair sequences of the forward and reverse primers was solved (using the Quick Change Manual Website) such that the melting temperature of the primer did not fall below 78 degrees. Using sterile lab practices, 43µL of double distilled water, 1µL of a 10mM DNTP, 1µL of a 50 ng/µL template DNA, and 1.25µL of a 100ng/µL primer mix, 1µL of enzymes, and lastly 25µL of a wax compound were added together. These were placed in the thermocycler and run according to the cycles solved using the Quick Change Manual. | |||
==Data== | ==Data== | ||
Revision as of 08:30, 21 September 2011
 Biomaterials Design Lab
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Entry title
ObjectiveDescriptionThe base pair sequences of the forward and reverse primers was solved (using the Quick Change Manual Website) such that the melting temperature of the primer did not fall below 78 degrees. Using sterile lab practices, 43µL of double distilled water, 1µL of a 10mM DNTP, 1µL of a 50 ng/µL template DNA, and 1.25µL of a 100ng/µL primer mix, 1µL of enzymes, and lastly 25µL of a wax compound were added together. These were placed in the thermocycler and run according to the cycles solved using the Quick Change Manual. Data
NotesThis area is for any observations or conclusions that you would like to note.
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