User:Noppadon Sathitsuksanoh

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Tik1             Noppadon Sathitsuksanoh (Tik)             Ph.D. candidate             200 Seitz Hall             Biological Systems Engineering Department             Virginia Tech             Blacksburg, VA 24061             sathino@vt.edu Primary Research Establishment and application of new genetic engineering tools and expression/secretion systems in Bacillus subtilis, Geobacillus and Brevibacillus brevis. Metabolism and metabolic engineering of Bacillus subtilis. Enzyme Engineering by directed evolution: A novel powerful and highly efficient Bacillus subtilis platform for cellulase engineering and performance improvement towards natural cellulose substrate has been established. Construction of recombinant consolidated bioprocessing (CBP) microorganisms (e.g., Bacillus, Geobacillus and yeast) for one-step production of biocommodities from renewable lignocellulosic materials. Synthetic biology and metabolic engineering for biofuel production from biomass. Microbiological resources and in situ biodegradation. Selected Publications Zhang, X.-Z., Sathitsuksanoh, N.,Zhu, Z. and Zhang, Y.-H. P. (2011) One-step production of lactate from cellulose as sole carbon source without any other organic nutrient by recombinant cellulolytic Bacillus subtilis, under review. Myung, S., Zhang, X.-Z., and Zhang, Y.-H. P. (2011) Ultra-stable phosphoglucose isomerase through immobilization of cellulosebinding module-tagged thermophilic enzyme on low-cost high-capacity cellulosic adsorbent, Biotechnology Progress In Press Zhang, X.-Z., and Zhang, Y.-H. P. (2011) Simple, fast and high-efficiency transformation system for directed evolution of cellulase in Bacillus subtilis, Microbial Biotechnology 4, 98-105. Zhang, X.-Z., Zhang, Z., Zhu, Z., Sathitsuksanoh, N., Yang, Y., and Zhang, Y.-H. P. (2010) The non-cellulosomal family 48 cellobiohydrolase from Clostridium phytofermentans ISDg: heterologous expression, characterization, and processivity, Applied Microbiology and Biotechnology 86, 525-533. Zhang, X.-Z., and Zhang, Y.-H. P. (2010) One-step production of biocommodities from lignocellulosic biomass by recombinant cellulolytic Bacillus subtilis: Opportunities and challenges, Engineering in Life Sciences 10(5):398-406. Zhang, X.-Z., Sathitsuksanoh, N., and Zhang, Y.-H. P. (2010) Glycoside hydrolase family 9 processive endoglucanase from Clostridium phytofermentans: heterologous expression, characterization, and synergy with family 48 cellobiohydrolase, Bioresource Technology 101, 5534-5538. Liu, W., Zhang, X.-Z., Zhang, Z., and Zhang, Y.-H. P. (2010) Engineering of Clostridium phytofermentans endoglucanase Cel5A for improved thermostability, Appl. Environ. Microbiol. 76, 4914-4917. Zhang, X.-Z., Yan, X., Cui, Z. L., Hong, Q., and Li, S. P. (2006) mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis, Nucleic Acids Res 34, e71. Zhang, X.-Z., Cui, Z.-L., Hong, Q., and Li, S.-P. (2005) High-level expression and secretion of methyl parathion hydrolase in Bacillus subtilis WB800, Appl. Environ. Microbiol. 71, 4101-4103. Cui, Z.-L., Zhang, X.-Z., Zhang, Z.-H., and Li, S.-P. (2004) Construction and application of a promoter trapping vector with methyl parathion hydrolase gene mpd as the reporter, Biotechnology Letters 26, 1115-1118.