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| [[Image:Gel_Box_2.0_Stylized.jpg|left]]<br/> | | page moved, please see [[DIYbio:Notebook/Open_Gel_Box_2.0|DIYbio Open_Gel_Box_2.0]] for the latest information |
| <html><a rel="license" href="http://creativecommons.org/licenses/by-sa/3.0/us/"><img alt="Creative Commons License" style="border-width:0" src="http://i.creativecommons.org/l/by-sa/3.0/us/88x31.png" /></a><br />This work is licensed under a <a rel="license" href="http://creativecommons.org/licenses/by-sa/3.0/us/">Creative Commons Attribution-Share Alike 3.0 License</a></html>
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| == Introduction ==
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| Gel electrophoresis is one of the most basic and commonly used tool for molecular biology. However, the gel electrophoresis apparatus available on the market often lack essential features that would make more pleasant for performing this daily routine. We aim to create an open and extensible electrophoresis gel box, available as design documents, unassembled, and assembled kits for researchers to obtain and improve upon.
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| == Summary ==
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| * Creative Commons Attribution-Share Alike 3.0 License
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| * Simple, minimalist design
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| * Inexpensive, using off the shelf parts
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| * Better features and improvements over electrophoresis gel boxes currently available on market
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| ** Built In/Swappable excitation light source and corresponding excitation/emission filter
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| ** Enable in-box gel manipulation by adjustable buffer emersion height
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| == Specification ==
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| # Open Source Design
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| ## Simple enough design so anyone can build it and improve upon it by having the following
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| ### Materials
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| #### Acrylic Sheets
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| #### Acrylic Glue
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| #### Platinum Wire
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| #### High voltage Wires (red/black)
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| #### Electrical Connectors
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| ### Tools/Skill
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| #### Acrylic cutting
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| #### Acrylic gluing
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| #### Needle-Nose Pliers
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| ## Modification of design are required to be shared-alike
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| # Capable of running electrophoresis
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| ## Molecules: DNA
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| ## Medium: Agarose concentration from 0.8% to 4.0%
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| ## Buffers: TAE, TBE, SB, LB, LA
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| ## Voltages: 0-350V
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| ## Stain: EtBr, SYBR Safe
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| # Safety
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| ## Fool-proof voltage disconnect upon lid removal
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| ## No offgas & evaporation build-up
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| # Casting
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| ## In-Box gel casting
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| ## Optional multi-gel casting tray (see Modular Extension)
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| # Visualization
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| ## Excitation Source
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| ### Built-in/Swappable excitation LED
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| ### Built-in/Swappable excitation filter
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| ## Emission Filtering
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| ### Built-in/Swappable emission filter
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| # In-Box On-Gel DNA Manipulation
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| ## Band stabbing
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| ## Band cutting
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| ## Dialysis tube/silica membrane band extraction
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| # Modular Extension
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| ## Camera mount
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| ## Buffer cooling ports
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| ## Multi-Gel casting tray
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| == Methods ==
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| * Excitation Source & Filter Selection
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| * Emission Selection
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| == References ==
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