User:Norman Wang/Open Gel Box 2.0
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Introduction
Specification
- Open Source Design
- Simple enough design so anyone can build it and improve upon it by having the following
- Materials
- Acrylic Sheets
- Acrylic Glue
- Platinum Wire
- High voltage Wires (red/black)
- Tools/Skill
- Acrylic cutting
- Acrylic gluing
- Needle-Nose Pliers
- Materials
- Modification of design are required to be shared-alike
- Simple enough design so anyone can build it and improve upon it by having the following
- Capable of running electrophoresis
- Molecules: DNA
- Medium: Agarose concentration from 0.8% to 4.0%
- Buffers: TAE, TBE, SB, LB, LA
- Voltages: 0-350V
- Stain: EtBr, SYBR Safe
- Safety
- Fool-proof voltage disconnect upon lid removal
- No offgas & evaporation build-up
- Gel Casting
- In-Box casting
- Optional multi-gel casting tray (see Modular Extension)
- Light
- Stain Excitation
- Built-in/Swappable excitation LED
- Built-in/Swappable excitation filter
- Stain Emission
- Built-in/Swappable emission filter
- Stain Excitation
- In-Box On-Gel DNA Manipulation
- Band stabbing
- Band cutting
- Dialysis tube/silica membrane band extraction
- Modular Extension
- Camera mount
- Buffer cooling ports
- Multi-Gel casting tray