User:Norman Wang/Open Gel Box 2.0

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Introduction

Specification

  1. Open Source Design
    1. Simple enough design so anyone can build it and improve upon it by having the following
      1. Materials
        1. Acrylic Sheets
        2. Acrylic Glue
        3. Platinum Wire
        4. High voltage Wires (red/black)
      2. Tools/Skill
        1. Acrylic cutting
        2. Acrylic gluing
        3. Needle-Nose Pliers
    2. Modification of design are required to be shared-alike
  2. Capable of running electrophoresis
    1. Molecules: DNA
    2. Medium: Agarose concentration from 0.8% to 4.0%
    3. Buffers: TAE, TBE, SB, LB, LA
    4. Voltages: 0-350V
    5. Stain: EtBr, SYBR Safe
  3. Safety
    1. Fool-proof voltage disconnect upon lid removal
    2. No offgas & evaporation build-up
  4. Gel Casting
    1. In-Box casting
    2. Optional multi-gel casting tray (see Modular Extension)
  5. Light
    1. Stain Excitation
      1. Built-in/Swappable excitation LED
      2. Built-in/Swappable excitation filter
    2. Stain Emission
      1. Built-in/Swappable emission filter
  6. In-Box On-Gel DNA Manipulation
    1. Band stabbing
    2. Band cutting
    3. Dialysis tube/silica membrane band extraction
  7. Modular Extension
    1. Camera mount
    2. Buffer cooling ports
    3. Multi-Gel casting tray