User:Nouf Abuladel/Notebook/CHEM 472/2016/02/02

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Objective Setting up the camera and light inside the oven. Also, preparing a lysozyme stock solution to make the fiber solution. Procedure 1) reading instruction to initiate the camera 2) making lysozyme stock solution and calculate the volume of gold, water and Lysozyme that should be added to the 20-mL glass tube. 3) doing some trial to figure out the best position of light, camera and fiber solution in the oven. 4) turning on the camera to record the formation of fibers overnight in the oven. Below are the calculations for today's fiber synthesis. In addition to this reaction preparation Gold Stock   MW HAuCl4*H2O (g/mol) 339.79 Mass used (g) 0.05048 Moles used (mol) 0.000148562 Volume stock (L) 0.05 Concentration of stock (mol/L) 0.002971247 Target concentration (mol/L) 0.00025 Target volume of final (mL) 10 Volume stock needed for final (mL) 0.841397583     Lysozyme Stock   MW Lysozyme (g/mol) 14307 Mass used (g) 0.03065 Moles used (mol) 2.14231E-06 Volume stock (L) 0.1 Concentration of stock (mol/L) 2.14231E-05 Target concentration (mol/L) 0.000004167 Target volume of final (mL) 10 Volume stock needed for final (mL) 1.945098499     Water   Volume water needed for final (mL) 7.213503918 The final setup of the camera in the oven. Data The fibers worked but the camera did not. This is because the battery was dead by the time we took the camera out of the oven. In order for the files to be saved, we needed to press the off button. Observations NOTHING WAS OBSERVED SINCE WE DID NOT HAVE RECORDS.