Dominic Schmidt
Doctoral Student
Darwin College, Department of Oncology, University of Cambridge
Cancer Research UK, Cambridge Research Institute
Li Ka Shing Centre
Robinson Way, Cambridge, CB2 0RE
phone: +44-1223404248
email: Dominic.Schmidt 'at' cancer 'dot' org 'dot' uk
We recently showed that cohesin co-binds across the genome with transcription factors independently of CTCF, plays a functional role in estrogen-regulated transcription, and may help mediate tissue-specific transcriptional responses via long-range chromosomal interactions.
Myriad points of control influence gene expression; however, it has also
been an unresolved question as to which of these mechanisms has the most
influence globally. We recently showed that each layer of transcriptional regulation
within the adult hepatocyte, from the binding of liver master regulators and
chromatin remodelling complexes to the output of the transcriptional machinery, is
directed primarily by DNA sequence. Although conservation of motifs alone cannot
predict transcription factor binding, we show that within the genetic sequence there
must be embedded adequate instructions to direct species-specific transcription:
We published a detailed protocol for ChIP-seq for whole tissues and cell lines. Furthermore we compared the influence of sequencing depth on peak calling using matched ChIP-chip data from the identical sequencing libraries:
I received my german diplom degree in Biochemistry at the Max Planck Institute for Molecular Genetics in the department for Vertebrate Genomics of Hans Lehrach in the laboratory of Marie-Laure Yaspo. The focus of my research was the analysis of gene regulatory networks, especially for human chromosome 21 encoded transcription factors. For this purpose I worked with ChIP-chip using high density and developed ChIP-seq using next generation sequencing technology. We published ChIP-seq for RNA-polymerase II in human cells together with RNA-seq: