User:Orsolya Kiraly

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===Books===
===Books===
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[http://books.google.com/books?id=WYCJBzTxPywC&printsec=frontcover&dq=suffering+gene&hl=en&ei=0ciBTL3nG4P98Ab2vb2aAg&sa=X&oi=book_result&ct=result&resnum=1&ved=0CCUQ6AEwAA#v=onepage&q&f=false The Suffering Gene]
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[http://books.google.com/books?id=5ziWNCqD7OEC&printsec=frontcover&dq=cancer+evolutionary&hl=en&ei=I8mBTPKHGoOC8gbav6H3AQ&sa=X&oi=book_result&ct=result&resnum=5&ved=0CEIQ6AEwBA Cancer, the evolutionary legacy]
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[http://books.google.com/books?id=WGmVPGh7AOMC&printsec=frontcover&dq=correcting+the+blueprint+of+life&source=bl&ots=6XcQhpeXhL&sig=6B-9fzLtn4vZO6swtR-WTc8TgnA&hl=en&ei=IjmCTNCRJ8P-8Aa_hPWBAg&sa=X&oi=book_result&ct=result&resnum=1&ved=0CBIQ6AEwAA#v=onepage&q&f=false Correcting the Blueprint of Life: An Historical Account of the Discovery of DNA Repair Mechanisms]
===Miscellaneous===
===Miscellaneous===

Revision as of 07:20, 4 September 2010

Contents

Contact Info

  • Orsolya Kiraly
  • Postdoctoral Associate, Engelward laboratory
  • Department of Biological Engineering
  • Massachusetts Institute of Technology
  • 77 Massachusetts Ave, Cambridge, MA
  • Lab: 617-750-7335 (Room 16-760)
  • Email me through OpenWetWare


Education


Research interests

I received my PhD for work on how rare mutations in pancreatic trypsin inhibitor contribute to chronic pancreatic inflammation. In the Engelward lab, my current work is aimed at homologous recombination in the pancreas in vivo.

While mitotic homologous recombination is an important DNA repair/tolerance mechanism, it can result in sequence rearrangements that can contribute to cancer. I am investigating the effects of DNA damaging chemicals, radiation, cell proliferation and DNA repair on homologous recombination in the pancreas.

The next question in my project is whether homologous recombination is induced by inflammation, which is a major risk factor for cancer.

Past projects

In the lab of Miklos Sahin-Toth at Boston University, I was investigating the functional effects of mutations in SPINK1, the pancreatic secretory trypsin inhibitor. This inhibitor is an important line of defense against trypsin activity in the pancreas. It is important to inhibit any trypsin activity in the pancreas because trypsin activity can result in the activation of other digestive enzymes in a cascade reaction, which can lead to cell damage and pancreatic inflammation. We found that signal peptide mutations abolish the secretion of SPINK1 into pancreatic juice, and coding region mutations cause misfolding of the protein which is degraded intracellularly and is not secreted. In patients with these mutations, spontaneously activated trypsin is thus not inhibited by SPINK1, eventually resulting in autodigestion and inflammation.


At Semmelweis University I was working in a team studying the functional effects of polymorphisms in the promoter of the D4 dopamine receptor gene. This gene was the first one to be investigated in psychiatric genetics association studies, and its polymorphisms are associated with several personality traits and disorders such as ADHD. However, the functional effects of promoter polymorphisms (and thus the molecular basis of these associations) was not clear. With a reporter gene assay, we found that a duplication in the promoter decreases transcriptional efficiency, potentially influencing the number of receptor molecules and neurotransmission. However, the most widely studied SNP in the gene had no effect on gene expression in our assay. The apparent effect of this SNP in association studies is thus probably due to another variant which is in linkage disequilibrium with the candidate SNP.


My undergraduate thesis project at the Agricultural Biotechnology Research Center (in Gödöllő, Hungary) was aimed at generating host factor independent mutants of the 16-3 phage integrase by protein engineering. Integrases catalyze site-specific recombination which is harnessed in gene targeting. This project and the excellent mentoring I received at ABC gave me strong foundations in laboratory techniques in molecular biology and an interest in DNA metabolism and recombination.


Publications

Research papers

  1. Kereszturi E, Király O, and Sahin-Tóth M. . pmid:18978175. PubMed HubMed [Paper1]
  2. Király O, Wartmann T, and Sahin-Tóth M. . pmid:17525091. PubMed HubMed [Paper2]
  3. Király O, Boulling A, Witt H, Le Maréchal C, Chen JM, Rosendahl J, Battaggia C, Wartmann T, Sahin-Tóth M, and Férec C. . pmid:17274009. PubMed HubMed [Paper3]
  4. Kereszturi E, Kiraly O, Csapo Z, Tarnok Z, Gadoros J, Sasvari-Szekely M, and Nemoda Z. . pmid:17171658. PubMed HubMed [Paper4]
  5. Kereszturi E, Kiraly O, Barta C, Molnar N, Sasvari-Szekely M, and Csapo Z. . pmid:16723017. PubMed HubMed [Paper5]
  6. Király O, Guan L, Szepessy E, Tóth M, Kukor Z, and Sahin-Tóth M. . pmid:16542853. PubMed HubMed [Paper6]
  7. Szantai E, Kiraly O, Nemoda Z, Kereszturi E, Csapo Z, Sasvari-Szekely M, Gervai J, and Ronai Z. . pmid:16314756. PubMed HubMed [Paper7]
All Medline abstracts: PubMed HubMed

Book chapters

Nemoda Z, Kiraly O, Barta C, Sasvari-Szekely M. Pharmacogenetic aspects of dopaminergic neurotransmission-related gene polymorphisms. In: Darvas F, Guttman A, Dormán G (eds): Chemical Genomics, Marcel Dekker Inc., New York, 2003, pp. 275-313. isbn=0824754905

Király O, Guan L, Sahin-Tóth M. Expression of recombinant proteins with uniform N termini using intein technology and aminopeptidase deficient Escherichia coli. In: Ming-Qun X, Evans T (eds): Heterologous Protein Expression in E. coli, Methods in Molecular Biology, Springer/Humana Press, in press


Bookmarks

Laboratory tools and resources

Qiagen bench guide

Centrifuge calculator

Berglund:PCR_Additives A good list of PCR additives

GeneCards

iHOP


Useful links

Classification of carcinogens according to the International Agency for Research on Cancer

Environmental Working Group

The Comparative Toxicogenomics Database

American Association for Cancer Research


Books

The Suffering Gene

Cancer, the evolutionary legacy

Correcting the Blueprint of Life: An Historical Account of the Discovery of DNA Repair Mechanisms


Miscellaneous

Personal tools