User:Pakpoom Subsoontorn/Notebook/Genetically Encoded Memory

Notes

• Our review paper on genetically encoded memory (in preparation)
• (to be removed) summary note of site-specific recombinases
• List of site-specific recombinases
• Assay for site-specific recombinase profiling
• Presentation: writing to DNA (present to Infinite Monkey Theorem Group, 11/01/98)

Links

• labs that work on recombinases

Outline

1. Application
2. Grand Challenges
3. Start-up experiments
4. Review Articles

Project List

• Synthetic Jumping DNA from Integrase/excisionase system
• Implementing N-bit memory with DNA recombination
• Engineering cell-cycle reporter
• Quantitative functional profiles (Database) of site-specific recombinase
• Expanding Integrase/excisionase library

Conceptual questions

• In the abstract level, how could we live with imperfect efficiency? No matter writing mechanism we choose in the end, the fraction of cells whose genome is actually rewritten will likely to be significantly less than a hundred percent.
• What are the common/ different challenges between writing memory to DNA versus gene therapy/ transgenic?
• Why reaction of some DNA integration is irreversible but most other don't?

Recent changes