User:Pakpoom Subsoontorn/Notebook/Genetically Encoded Memory/2008/10/05: Difference between revisions

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==Thought==
==Thought==
* Insert content here...
* Φc31 (and other serine recombinase) seems to be good choices for modify DNA code in term of efficiency and simplicity. They need only ~30 sth recognition sites on the inserting and the target DNA; no host recombination factor seems to be required; it has been shown to function in vitro and in non-natural host ...like animal and plant cell.
* One drawback is that their structures are poorly known and there is no known excision system yet.
 
==Management Questions/requests==
==Management Questions/requests==
* Should we push the review article on Genetically Encoded Memory to general
* Should we push the review article on Genetically Encoded Memory to general

Revision as of 23:39, 5 October 2008

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Thought

  • Φc31 (and other serine recombinase) seems to be good choices for modify DNA code in term of efficiency and simplicity. They need only ~30 sth recognition sites on the inserting and the target DNA; no host recombination factor seems to be required; it has been shown to function in vitro and in non-natural host ...like animal and plant cell.
  • One drawback is that their structures are poorly known and there is no known excision system yet.

Management Questions/requests

  • Should we push the review article on Genetically Encoded Memory to general

audiences? or just leave it as a note among ourselves?

  • As we discussed earlier, there are 3-4 levels of the project: The application level (like engineering a yeast to report its age, or an in vivo directed evolution system), the grand-challenge level (like making 8-bit counter) and the specific experiment level. How should we manage our time?
  • Relating to the question above, should I start a deep-research on a particular system (say, lambda integrase)? or should I explore broader ranges of tools (other enzymes, recombination system etc.) for now?

Science Question

  • Insert content here...


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