User:Pakpoom Subsoontorn/Notebook/Genetically Encoded Memory/2008/10/06: Difference between revisions

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==mini-Project: Reversible Genetic Knockout==
==mini-Project: Synthetic Jumping DNA from integrase/excisionase systems==
* The genetic systems has two states: A, B, C. The state can be changed  
* The genetic systems has two states: A, B, C. The state can be changed  
upon the expression of two different intergrase/excisionase systems.
upon the expression of two different intergrase/excisionase systems.

Revision as of 21:38, 6 October 2008

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mini-Project: Synthetic Jumping DNA from integrase/excisionase systems

  • The genetic systems has two states: A, B, C. The state can be changed

upon the expression of two different intergrase/excisionase systems.

  • In state A, the cell has two pieces of DNA, the chromosomal DNA and

the free circular plasmid. The chromosomal DNA has two different bacterial integration sites for integrase, attB-1 and attB-2. The plasmid also has two different phage-integration sites for integrase, attP-1 and attP-2.

  • The expression of the integrase-1 will insert attP-1 into attB-1, changing

the system from state-A to state-B. On the other hand, the expression of integrase-2 will insert attP-2 into attB-2, changing the system from state-A to state-C

  • The inserted piece of DNA can removed with excisionase system. Thus, we could

reverse state-B or state-C back to state-A again.

figure1