User:Pakpoom Subsoontorn/Notebook/Genetically Encoded Memory/2008/10/11: Difference between revisions

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==Experiment setup==
==Experiment setup==
==Questions==
==Questions==
*What're the general limitations of protein evolution? What could be the particular limitations in evolving recombinases? Library site? Screening?
==TO DO list==
==TO DO list==
==Reference==
==Reference==

Revision as of 20:21, 16 October 2008

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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miniProject: Expanding integrase/excisionase library

  • For this project, we will evolve a large library of integrase/excisionase system whose

att sequences do not exist in nature. The experiment will involve protein evolution, high-throughput screening assay and bioinformatics. We also want to learn what set of sequence cannot be used as integrase target.

Project outline

Experiment setup

Questions

  • What're the general limitations of protein evolution? What could be the particular limitations in evolving recombinases? Library site? Screening?

TO DO list

Reference


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