User:Pakpoom Subsoontorn/Notebook/Genetically Encoded Memory/2009/03/29: Difference between revisions
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**Mechanism: IPTG diffuses across E.coli cell membrane and cytosol and binds to lacI | **Mechanism: IPTG diffuses across E.coli cell membrane and cytosol and binds to lacI | ||
**Rate | **Rate | ||
***Diffusion rate of IPTG across cell membrane | |||
***Diffusion rate of IPTG in cytosol | |||
***IPTG-lacI binding rate | |||
**Number | **Number | ||
***number of lacI in a single cell | |||
*lacI-IPTG leaves the promoter | *lacI-IPTG leaves the promoter | ||
**Mechanism | **Mechanism: lacI-IPTG has much lower affinity toward Lac operator than lacI and likely to dissociate | ||
**Rate | **Rate | ||
***Dissociation rate of lacI-IPTG from the lac operon | |||
**Number | **Number | ||
*Transcription of Flipper | *Transcription of Flipper | ||
**Mechanism | **Mechanism: RNAP bind to the promoter, transcribing mRNA of the flipper out | ||
**Rate | **Rate | ||
***Estimated transcription rate | |||
***promoter occupancy of our promoter | |||
**Number | **Number | ||
***Size of the gene | |||
***number of RNAP molecules | |||
*Translation of Flipper | *Translation of Flipper | ||
**Mechanism | **Mechanism |
Revision as of 22:13, 28 March 2009
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html> |
Modeling and measurements for flipper operation
Detail Mechanism
Measurement(Currently available in our lab)
(Not available in our lab yet)
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