User:Pakpoom Subsoontorn/Notebook/Shape Determination in Bacteria/2008/10/17: Difference between revisions

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* Skim through the first three chapters of AutoCAD bible
* Skim through the first three chapters of AutoCAD bible
==20/24/08==
*Start reading "Cell shape dynamics in E.coli" by Reshes G, et al 2007
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Revision as of 10:01, 27 October 2008

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Lab note: 10/17/08-10/23/08

  • In summary,

10/17/08

  • Make an agar slide of E.coli (K-12 wild type) cell that has been cultured for about 24 hours at 37 C in 5 ml plain LB.
  • The slide is made from glass slide, cover slid, newly-made LB agar (20 ml LB+0.3gram of agar, heated up on hot plate with stirrer). About 1.5 ul of cultured E.coli is dropped on the agar patch directly without dilution. After the cultured solution is dried, put cover slid on and sealed with... (see image)
  • Take phase-contrast image at 4 different positions, looping every 10 seconds for 360 cycles. Exposure time is

10/21/08

  • Meet with Tony: neofilm sandwich and laser cutter for cell-flow template
  • Start Cultured E.coli K-12 in 5 ml LB 37 C at 10 am. Streak a new plate (plain LB) of E.coli K-12 from Steph's stock, grow at 37 C
  • Install AutoCAD 2008 in macbook

20/22/08

  • Start Cultured E. K-12 in 5 ml LB 37 C at 8.30 am from 10/21/08 stock. Note: no gas for burner!

20/24/08

  • dilute FM464 dye 1:200 from stock (1 mg/ml)
  • start drop cell 9 am : 1 ul of over night culture + 2 ul of dye
  • emission filter, Nikon 59004 (C93105), 49002 (C91306), 49004 (C91530)
  • finish the first draft of the simple microfluidic design and gave it to KC

SimpleMask1

  • Skim through the first three chapters of AutoCAD bible

20/24/08

  • Start reading "Cell shape dynamics in E.coli" by Reshes G, et al 2007


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