User:Patrick Hampson/Notebook/chem471/2016/09/20

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Fluorescence 2016920

Sample Preparation

BSA Fluorescence Solution

  1. pH 5
  2. [Au]=0.25 mM
  3. [BSA]=3.125 uM
  4. Vf=3000 uL

Stock Concentrations 160920

  1. [BSA]= 49.1 uM
  2. [Au]= 3.73 mM
  3. [HCl]= 1mM

Volumes for making the solution:

  1. 30 uL HCl
  2. 190.936 uL BSA
  3. 147.63 uL Au
  4. 2631.42 uL H20

Results

This graph shows the Fluorescence intensity expelled from the pH 5 sample (0.25 mM Au, 3.125 BSA) over time (as it was heated by the machine). It is apparent that the fluorescence intensity increases in time, as the sample was heated.

This graph illustrated the emission maximums over time. The emissions increased until about 20 minutes into the experiment. After that the maximum emission was fluctuating just above 420 nm.

The graph above shows the integrated intensity of the sample over time. The curve appears to take a logarithmic patter. The Fluorescence increases quickly in the initial 20 minutes, reaching ~7400 nm. After that the Fluorescence continues to increase, but at a significantly slower rate, barely exceeding 9000 nm by the end of the experiment.

UV-Vis Sample Preparation for tomorrow

Stock Solutions:

  1. 14.8 mM Fructose
  2. 49.1 mM BSA
  3. 3.73 mM Au
  4. 1mM HCL
  5. 1mM NaOH
  6. 1M NaOH


0.75 mM Fructose
pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of BSAstock μL of Water
4 500 0 0 335 253 316 3596
5 50 0 0 335 253 316 4046
6 5 0 0 335 253 316 4091
7 0 0 0 335 253 316 4096
8 0 5 0 335 253 316 4091
9 0 50 0 335 253 316 4046
10 0 500 0 335 253 316 3596
11 0 0 5 335 253 316 4091
12 0 0 50 335 253 316 4046


1.25 mM Fructose
pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of BSAstock μL of Water
4 500 0 0 335 422 316 3427
5 50 0 0 335 422 316 3877
6 5 0 0 335 422 316 3922
7 0 0 0 335 422 316 3927
8 0 5 0 335 422 316 3922
9 0 50 0 335 422 316 3877
10 0 500 0 335 422 316 3427
11 0 0 5 335 422 316 3922
12 0 0 50 335 422 316 3877

After the samples were prepared, they were left to cook in the thermooven at 80 degrees celcius for four hours.


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