User:Patrick Hampson/Notebook/chem471/2016/10/05

From OpenWetWare
Jump to navigationJump to search
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Ocean Optics 20161005

  • *Our samples were run without the heater turned to 80C, and were recreated and ran the next day.

Sample Preparation

BSA Ocean Optics Final Sample Solution Concentrations:

  1. 3 mL total
  2. 0.25 mM Au
  3. 3.125 μM BSA
  4. HCl or NaOH appropriate for your pH (7 today)

Stock solution Concentrations:

  1. 3.73 mM AuCl3
  2. 46.5 mM BSA
  3. 1 mM HCl
  4. DI H20

Solution Transfer Volumes:

  1. 2597 uL H20
  2. 201.6 uL AuCl3
  3. 201.0 uL BSA
  4. 0.3 uL HCl

Data

This figure displays the maximum absorbance over time of our pH 7 Au NP solution. When compared to older figures, it appears that pH 7 has yielded a much lower number of nanoparticles. Though the amount yielded in 3 hours has decreased, there is still an upward trend in nanoparticle formation when the run was stopped.

This figure displays the absorbance at wavelength 535.15 nm over time of our pH 7 Au NP solution. When compared to older figures, it appears that pH 7 has yielded a much lower number of nanoparticles. Though the amount yielded in 3 hours has decreased, there is still an upward trend in nanoparticle formation when the run was stopped.

The graph above shows a steady reading of approximately 450 nm until about 7000 seconds, where there is an increase in the maximum absorbance to approximately 540 nm. The readings, while they have plateaued after the initial spike, fluctuate around 540.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Patrick_Hampson/Notebook/chem471/2016/10/05&oldid=959697"