User:Patrick Hampson/Notebook/chem471/2016/11/29

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Fluorescence pH11

Sample Preparation

  1. [Au]=0.25 mM
  2. [BSA]=3.125 uM
  3. Vf=3000 uL

Stock Concentrations

  1. [BSA]= 30.4 uM
  2. [Au]= 3.37 mM
  3. [NaOH]= 1 M

Volumes used:

  1. 308.38 uL BSA
  2. 225.55 uL Au
  3. 2466.06 uL H2O
  4. 3.00 uL NaOH

Data

The above graph shows our emission maximum for our pH 11 solution. There really is no major change over time. It remains constant at just over 400 nm, but could potentially be trending upwards with time.

There is a steep initial increase from 12000 to around 37000 within 10000 seconds. From there, it trends upward constantly, reaching just under 40000 at the end of the run.

The graph above is the emission intensity over time for our 0-900 second scans. As we have seen in previous trials, this is where largest change in emission intensity occurs around our wavelength of interest. The emission intensity jumps from approximately 10 to over 30 in the 900 seconds span. There a minor shift to the right from the first to second trial, but after that there was no detectable shift.

The graph above is the emission intensity over time for our 1080-4500 second scans. There is nearly no change over time in our fluorescence activity. The peak is not as refined as it is in the 4680-10800 scans.

The graph above is the emission intensity over time for our 4680-10800 second scans. As observed in the 1080-4500 scans, there was no change in fluorescence peak activity around 410.


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