User:Patrick Hampson/Notebook/chem471/2016/12/07: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==Fluorescence pH12==
* Insert content here...
*  
==Sample Preparation==


# [Au]=0.25 mM
# [BSA]=3.125 uM
# Vf=3000 uL
Stock Concentrations
# [BSA]=  30.4 uM
# [Au]= 3.37 mM
# [NaOH]= 1 M
Volumes used:
#  308.38 uL BSA
#  225.55 uL Au
#  2439.06 uL H2O
#  30.00 uL NaOH
==Data==
[[Image:Fluorph11emissionamf.PNG]]
The graph above displays our emission maximum over the duration of the experiment. It is consistent throughout the trial.
[[Image:Fluorph11integratedamf.PNG]]
The graph above displays the emission intensity. It increases (spike) initially, and continues to increase throughout the experiment, but the increase is very minor.
[[Image:Fluorph11scansparta.PNG]]
The graph above is from selected scans 0-900. This graph shows the transformation of fluorescence as the initial reactants and formed intermediates are converted into fluorescent products.
[[Image:Fluorph11scanspartb.PNG]]
The graph above is from selected scans 1080-4500. The peak has been established around 350+ but is still rigid.
[[Image:Fluorph11scanspartc.PNG]]
The graph above is from selected scans 4680-10800. The peak has solidified just above 350 nm.


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Latest revision as of 02:11, 27 September 2017

Project name Main project page
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Fluorescence pH12

Sample Preparation

  1. [Au]=0.25 mM
  2. [BSA]=3.125 uM
  3. Vf=3000 uL

Stock Concentrations

  1. [BSA]= 30.4 uM
  2. [Au]= 3.37 mM
  3. [NaOH]= 1 M

Volumes used:

  1. 308.38 uL BSA
  2. 225.55 uL Au
  3. 2439.06 uL H2O
  4. 30.00 uL NaOH

Data

The graph above displays our emission maximum over the duration of the experiment. It is consistent throughout the trial.

The graph above displays the emission intensity. It increases (spike) initially, and continues to increase throughout the experiment, but the increase is very minor.

The graph above is from selected scans 0-900. This graph shows the transformation of fluorescence as the initial reactants and formed intermediates are converted into fluorescent products.


The graph above is from selected scans 1080-4500. The peak has been established around 350+ but is still rigid.

The graph above is from selected scans 4680-10800. The peak has solidified just above 350 nm.


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