User:Patrick Wu: Difference between revisions
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* 2011, BS in Biological Engineering, MIT | * 2011, BS in Biological Engineering, MIT | ||
== | ==Module 2 Information== | ||
Designing forward and reverse primers to tag specific YGR067C protein at the 3’ end of DNA sequence | |||
TCCATGGAAAAGAGAAGATG - Tm 49.6 deg C | |||
Forward Primer | |||
5’CAAGAATTCTCAGCTTCTTCAACTGACAATAAACAAAGTTCCATGGAAAAGAGAAGATG3’ | |||
G/C content 33.9%, Tm 64.7 deg C | |||
TACGACTCACTATAGGGCGA – Tm 54.3 deg C | |||
Reverse Primer | |||
5’CTTTGGCCATTATTTTATTTGGCTAAAAATTTCAATGTTTACGACTCACTATAGGGCGA3’ | |||
G/C content 33.9%, Tm 65.4 deg C | |||
Sequence of YGR067C | |||
5’ | |||
ATGGCTGCGGGTCAAAAAAAATATATATGTTCCTTTTGTTTGAAGCCTTTCTCGAGATCC | |||
GAGCACAAGATAAGGCATGAAAGGTCTCATGCGGGCGTGAAGCCTTTCCAGTGCCAAGTC | |||
TGTAAACACTCTTTTGTTAGACGCGATCTCTTACAGCGACACATCAGGACTGTCCACAGA | |||
ACCTTCCTCTTATCCAGTTACGCGTCAATGACAGGCGATAAGGCTGATATCCCTGTATCA | |||
CTTGGCGTTGGTGACGTTGATTCGACTTCACCCAGAGATATCAAAATGGAAACACTCGTT | |||
AATTCGATGATTAAGGTCAACAGCGGCCTTATCAATATTCACTATCATAGTAGTAATGTT | |||
GAAAAAATGGATAAGCAGCAACGTTGTGTGATTGGGAAGGAGTCCTCTAGTTTAAAGAAA | |||
GGGAAAAGCCGCTTTAAGCAGGTTAAAAGCCGGTTGGAGTCCTCCATCAGTGTAAAAATT | |||
TTGCAAGAATATTCATTAGATTTTATTAGCTCAAGAGATATTCTAACTTTCTTCAGAATG | |||
GGTGTAAGCCACTTGGTAGAAAATAAGATCTTCCAAAATTTCTTTCCAGACCTGTTCTCA | |||
TCACTACAAAATGACGAGCTGGTGGAAAGTTTTTGGATTAACAAGCCTTTTGGTCTCATA | |||
ATAGCTTGTCTGGGTATGTCCATTTCGTTAAATCAAGATTCTCAAAAACTATGGTTTATC | |||
TGTTGTACGAATTTGTATGCTAGCTCATCTAAACATGACAATGACTTTGACACTGAGGAC | |||
ATTTTGTCACAGACAGAGCAACATGATGTTTTTGCCTTGATTCTATTTTATTCTCTTCTA | |||
GTAATGTTGGAGAATAACATTCCTGTTTCTAATTCCATTAAAAAGTTCGATGTGTTTTCA | |||
ATGCTTCAAGATATTCTGAAGCCATTCACCGTGGCATCCTCCAGTTACCATTATTTGAAT | |||
AGTAAGGAAAATGCATGGTTCATATTCGATCTATGGGTCAATATCTTGCGCGATTCAAAC | |||
AACTTCAACAACGATTCTTTACTAATATTTGGTTGGTTCGTCAATCAAGAGTTTATTTCG | |||
TCTAATCCACTTAAGGACTTCATATACAAGGGACCGTCAATGTCCACTACTGACTTAACT | |||
TTAAAGCATATCAATATTTTGGCAGATTCCGCGTACGTGTATTTTATAATAAAGAAGACT | |||
TATCCGCAAGAGCTGCCATCTGATTTTAGAGTGCATGATTTGCTGGTTTACTTGAATGAA | |||
TGTTTCGTAATGCAGCAGCCGATCAAACCTGAAACTAGTGCAAACCCCTCTTTATTTGCG | |||
AACGTTATGAACGCCAGGATTACGGACTGCAAATCGAAAAGTAACTGGTTATTGTGGGAA | |||
ACGATTTGGTTCGAATTCATCAACAACTTGACTTTGAGAAATGGGACCACAAGGAACATA | |||
TGGTTCATTGATAACTTTCCACAGGTATCGACCTCGTGCTTACTTCATCATTCTTCCTCA | |||
TTTGTTGATGAAACCTTGATCACCACGAACTTATCAATCATATCAATGCTTCTTAATTTG | |||
AAGTCTTTCACGCTGGCATCTTTAAACCCAAGGAATATTCAGTTAATTACCGACATTGTC | |||
AGCTTCCAACTAAAATTGTTTTCATCGGAGTTAATTGCAAGCTCGGATGTTTCACCATCA | |||
CAAGTATCTCAATTACTAGTAAACCCCAATGTTCACCTAATGTTATATTTCTGGTTCGAT | |||
ACCATTTATGTACAACGTCAGAGCTACCTTTCATCAACAGAGAAAGAGGAATTTGAGAAG | |||
GTTGAAGTGTTTGTGAATGACTACATTATAACACACCAAAAGAACTTGGTGACAGACTTA | |||
CACTCCATTTTGTTCGATTTTTGGTCAGATTCCTTCATTGCTTACCATATACTACTGCAT | |||
GCTATTGTATCTTCATTAAGAGATAATATTCTTTACCCATACTTAATCTACTCCCCTCAC | |||
CTGAATGACCAGACGAAAGCATTGTTGACTGATATCTCTAATTGGTCTTGTTTTGCTCTT | |||
CAACAGCCGTTTCGCAAGACTTCAAGAGGTTCTTTATCTGGTGCTTCTGATATGAAATCG | |||
TTTTCAGTAGCATCATGTTTGCCACTCTCTCCTAATTTCCTAAAGAGAGATTCAAACTGT | |||
AATAAGATTCTCCTGCCCCCATTAGATATAAAAGCCATTGAACCTATCTCAACGAGTAAT | |||
TACACCTACGTGAACTCGGCCCCTAAGCAACAAGAGAAGGAACAACCATTGTTAAGGGCA | |||
ACAGGAAACAATATCAATTTAGTCCAAACAATAGTGGTCCCACCCCAGGTAAACATGGAA | |||
TCACAAGAATTCTCAGCTTCTTCAACTGACAATAAACAAAGTTAA | |||
3’ | |||
base pairs following gene… | |||
5’AACATTGAAATTTTTAGCCAAATAAAATAATGGCCAAAG3’ | |||
==Publications== | ==Publications== |
Revision as of 11:22, 7 October 2008
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Contact Info
- Patrick Wu
- MIT
- Next House
- Email: p_wu [at] mit.edu
Registration/Questionnaire: 20.109 Fall 2008
Last Name
Wu
First Name
Patrick
Preferred name
Patrick
Course/Minor
20, maybe also 18, minor in 15
Year of Graduation
2011
p_wu AT mit DOT edu
Have you taken or are you taking...
7.05/5.07 (Biochemistry) - no
7.06 (Cell Biology) - no
7.02 (General Biology Lab) - no
5.310 (General Chemistry Lab) - no
Do you have any experience culturing cells (mammalian, yeast or microbial)? - No
Do you have any experience in molecular biology (electrophoresis, PCR, etc)? - No (actually, minimal)
Please briefly describe any previous laboratory experience
Worked in a labbish enviroment over the summer (not very formal, not a research lab)
Anything else you would like us to know?
nope
Education
- 2011, BS in Biological Engineering, MIT
Module 2 Information
Designing forward and reverse primers to tag specific YGR067C protein at the 3’ end of DNA sequence
TCCATGGAAAAGAGAAGATG - Tm 49.6 deg C
Forward Primer 5’CAAGAATTCTCAGCTTCTTCAACTGACAATAAACAAAGTTCCATGGAAAAGAGAAGATG3’ G/C content 33.9%, Tm 64.7 deg C
TACGACTCACTATAGGGCGA – Tm 54.3 deg C
Reverse Primer 5’CTTTGGCCATTATTTTATTTGGCTAAAAATTTCAATGTTTACGACTCACTATAGGGCGA3’ G/C content 33.9%, Tm 65.4 deg C
Sequence of YGR067C
5’ ATGGCTGCGGGTCAAAAAAAATATATATGTTCCTTTTGTTTGAAGCCTTTCTCGAGATCC GAGCACAAGATAAGGCATGAAAGGTCTCATGCGGGCGTGAAGCCTTTCCAGTGCCAAGTC TGTAAACACTCTTTTGTTAGACGCGATCTCTTACAGCGACACATCAGGACTGTCCACAGA ACCTTCCTCTTATCCAGTTACGCGTCAATGACAGGCGATAAGGCTGATATCCCTGTATCA CTTGGCGTTGGTGACGTTGATTCGACTTCACCCAGAGATATCAAAATGGAAACACTCGTT AATTCGATGATTAAGGTCAACAGCGGCCTTATCAATATTCACTATCATAGTAGTAATGTT GAAAAAATGGATAAGCAGCAACGTTGTGTGATTGGGAAGGAGTCCTCTAGTTTAAAGAAA GGGAAAAGCCGCTTTAAGCAGGTTAAAAGCCGGTTGGAGTCCTCCATCAGTGTAAAAATT TTGCAAGAATATTCATTAGATTTTATTAGCTCAAGAGATATTCTAACTTTCTTCAGAATG GGTGTAAGCCACTTGGTAGAAAATAAGATCTTCCAAAATTTCTTTCCAGACCTGTTCTCA TCACTACAAAATGACGAGCTGGTGGAAAGTTTTTGGATTAACAAGCCTTTTGGTCTCATA ATAGCTTGTCTGGGTATGTCCATTTCGTTAAATCAAGATTCTCAAAAACTATGGTTTATC TGTTGTACGAATTTGTATGCTAGCTCATCTAAACATGACAATGACTTTGACACTGAGGAC ATTTTGTCACAGACAGAGCAACATGATGTTTTTGCCTTGATTCTATTTTATTCTCTTCTA GTAATGTTGGAGAATAACATTCCTGTTTCTAATTCCATTAAAAAGTTCGATGTGTTTTCA ATGCTTCAAGATATTCTGAAGCCATTCACCGTGGCATCCTCCAGTTACCATTATTTGAAT AGTAAGGAAAATGCATGGTTCATATTCGATCTATGGGTCAATATCTTGCGCGATTCAAAC AACTTCAACAACGATTCTTTACTAATATTTGGTTGGTTCGTCAATCAAGAGTTTATTTCG TCTAATCCACTTAAGGACTTCATATACAAGGGACCGTCAATGTCCACTACTGACTTAACT TTAAAGCATATCAATATTTTGGCAGATTCCGCGTACGTGTATTTTATAATAAAGAAGACT TATCCGCAAGAGCTGCCATCTGATTTTAGAGTGCATGATTTGCTGGTTTACTTGAATGAA TGTTTCGTAATGCAGCAGCCGATCAAACCTGAAACTAGTGCAAACCCCTCTTTATTTGCG AACGTTATGAACGCCAGGATTACGGACTGCAAATCGAAAAGTAACTGGTTATTGTGGGAA ACGATTTGGTTCGAATTCATCAACAACTTGACTTTGAGAAATGGGACCACAAGGAACATA TGGTTCATTGATAACTTTCCACAGGTATCGACCTCGTGCTTACTTCATCATTCTTCCTCA TTTGTTGATGAAACCTTGATCACCACGAACTTATCAATCATATCAATGCTTCTTAATTTG AAGTCTTTCACGCTGGCATCTTTAAACCCAAGGAATATTCAGTTAATTACCGACATTGTC AGCTTCCAACTAAAATTGTTTTCATCGGAGTTAATTGCAAGCTCGGATGTTTCACCATCA CAAGTATCTCAATTACTAGTAAACCCCAATGTTCACCTAATGTTATATTTCTGGTTCGAT ACCATTTATGTACAACGTCAGAGCTACCTTTCATCAACAGAGAAAGAGGAATTTGAGAAG GTTGAAGTGTTTGTGAATGACTACATTATAACACACCAAAAGAACTTGGTGACAGACTTA CACTCCATTTTGTTCGATTTTTGGTCAGATTCCTTCATTGCTTACCATATACTACTGCAT GCTATTGTATCTTCATTAAGAGATAATATTCTTTACCCATACTTAATCTACTCCCCTCAC CTGAATGACCAGACGAAAGCATTGTTGACTGATATCTCTAATTGGTCTTGTTTTGCTCTT CAACAGCCGTTTCGCAAGACTTCAAGAGGTTCTTTATCTGGTGCTTCTGATATGAAATCG TTTTCAGTAGCATCATGTTTGCCACTCTCTCCTAATTTCCTAAAGAGAGATTCAAACTGT AATAAGATTCTCCTGCCCCCATTAGATATAAAAGCCATTGAACCTATCTCAACGAGTAAT TACACCTACGTGAACTCGGCCCCTAAGCAACAAGAGAAGGAACAACCATTGTTAAGGGCA ACAGGAAACAATATCAATTTAGTCCAAACAATAGTGGTCCCACCCCAGGTAAACATGGAA TCACAAGAATTCTCAGCTTCTTCAACTGACAATAAACAAAGTTAA 3’
base pairs following gene… 5’AACATTGAAATTTTTAGCCAAATAAAATAATGGCCAAAG3’