User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2014/08/27: Difference between revisions
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*Actual amount of Lysozyme: | *Actual amount of Lysozyme: | ||
* 50 mL of 0.25 mM HCl was made as a control | |||
Next, solutions were made with gold:protein ratios of 10:1 to 60:1. (See the table). The test tubes were placed in at 80°C for three hours, starting at 13:00. | |||
==Kinetics== | |||
0.5 mL of HAuCl<sub>4</sub> stock and 0.166 mL of Lysozyme stock were added to three test tubes for a 30:1 gold to protein ratio. 4.33 mL of 80°C water was added. Kinetics runs were done every 30 minutes with 2.5 mL of sample in plastic cuvette from 800-360 nm. Started at 13:35. | |||
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Revision as of 17:01, 27 August 2014
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Making HAuCl4 and Lysozyme Stocks
Next, solutions were made with gold:protein ratios of 10:1 to 60:1. (See the table). The test tubes were placed in at 80°C for three hours, starting at 13:00. Kinetics0.5 mL of HAuCl4 stock and 0.166 mL of Lysozyme stock were added to three test tubes for a 30:1 gold to protein ratio. 4.33 mL of 80°C water was added. Kinetics runs were done every 30 minutes with 2.5 mL of sample in plastic cuvette from 800-360 nm. Started at 13:35.
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