User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2014/09/02: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Mixing NaOH and Amino Acid Stock Solutions== | ==Mixing NaOH and Amino Acid Stock Solutions== | ||
Today, we | Today, we made 200 µM Amino Acid stock solutions of Arginine, Valine, Threonine, Phenylalanine, and Glutamic Acid. These were titrated with 0.15 M NaOH to acquire a titration curve | ||
'''Procedure''' | '''Procedure''' | ||
3 mg of NaOH was mixed into 500 mL H<sub>2</sub>O. Next, 0.5 mg of KHPht was added to 15-30 mL H<sub>2</sub>O, and titrated. | 3 mg of NaOH was mixed into 500 mL H<sub>2</sub>O. Next, 0.5 mg of KHPht was added to 15-30 mL H<sub>2</sub>O, and titrated until the equivalence point. | ||
'''Results''' | '''Results''' | ||
See [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/CHEM_571_Fall_2014/2014/09/03 here] for the titration curve results. | |||
Latest revision as of 00:13, 27 September 2017
Project name | Main project page Previous entry Next entry |
Mixing NaOH and Amino Acid Stock SolutionsToday, we made 200 µM Amino Acid stock solutions of Arginine, Valine, Threonine, Phenylalanine, and Glutamic Acid. These were titrated with 0.15 M NaOH to acquire a titration curve
3 mg of NaOH was mixed into 500 mL H2O. Next, 0.5 mg of KHPht was added to 15-30 mL H2O, and titrated until the equivalence point.
See here for the titration curve results.
References: 1. http://www.webqc.org/aminoacids.php
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