User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2014/09/10: Difference between revisions
From OpenWetWare
(Autocreate 2014/09/10 Entry for User:Paul_Rothenberg/Notebook/CHEM_571_Fall_2014) |
|||
Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==Bradford Assay for BSA and Lysozyme== | ||
Today, we ran a Bradford Assay to determine actual protein concentration in solutions of BSA and lysozyme. | |||
==Procedure== | |||
Stock solutions of 0.9% w/v NaCl and 50 mM Tris/50 mM NaCl were prepared. 1 mg/mL solutions of BSA and lysozyme were prepared with the saline in a total volume of 5 mL. Bradford reagent was prepared in a 1 in 4 dilution. A range of dilutions was prepared, at 1, 2, 5, 10, 15, and 20 µg/mL protein, 200 µL Bradford reagent, and Tris/NaCl buffer to bring the volume to 1 mL total. | |||
==Results== | |||
Beer's Law plots were constructed with the data. | |||
Revision as of 14:07, 10 September 2014
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Bradford Assay for BSA and LysozymeToday, we ran a Bradford Assay to determine actual protein concentration in solutions of BSA and lysozyme. ProcedureStock solutions of 0.9% w/v NaCl and 50 mM Tris/50 mM NaCl were prepared. 1 mg/mL solutions of BSA and lysozyme were prepared with the saline in a total volume of 5 mL. Bradford reagent was prepared in a 1 in 4 dilution. A range of dilutions was prepared, at 1, 2, 5, 10, 15, and 20 µg/mL protein, 200 µL Bradford reagent, and Tris/NaCl buffer to bring the volume to 1 mL total. ResultsBeer's Law plots were constructed with the data.
|